Sheep linkage mapping: RFLP markers for comparative mapping studies

Montgomery, G. W.; Penty, J. M.; Henry, Hannah M.; Sise, J. A.; Lord, E.A.; Dodds, K. G.; Hill, Daniel H.

doi:10.1111/j.1365-2052.1995.tb03252.x

Cited by 8 publications

(2 citation statements)

References 103 publications

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“…RFLPs for the ovine equivalents of albumin (ALB), annexin V (ANX5), bone morphogenetic protein 3 (BMP3), casein ct-S1 (CSNIS1), complement factor 1 (IF), epidermal growth factor (EGF), gonadotropin-releasing hormone receptor (GNRHR), GRO1 oncogene (melanoma growth stimulating activity ix; GR01), Hardy-Zuckerman 4 feline sarcoma viral (v-kit) oncogene homolog (KIT), phosphodiesterase cyclic GMP [3 polypeptide (PDE6B), platelet-derived growth factor receptor polypeptide (PDGFRA), and secreted phosphoprotein 1 (SPP1) have been described (Montgomery et al 1995a(Montgomery et al , 1995b. The RFLP for cyclin A (CCNA) was identified after hybridizing the human cDNA clone CycA (Pines and Hunter 1990) with Southern filters of sheep DNA samples digested with Taql.…”

Section: Rflp Analysismentioning

confidence: 99%

The linkage map of sheep Chromosome 6 compared with orthologous regions in other species

et al. 1996

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The genetic linkage map of sheep Chromosome (Chr) 6 has been extended to include 35 loci with the addition of 11 RFLP and 12 microsatellite loci. The sex-averaged linkage map now spans 154 cM from phosphodiesterase cyclic GMP beta polypeptide (PDE6B) to OarCP125, an anonymous sheep microsatellite. The male and female map lengths, at 180 cM and 132 cM respectively, did not differ significantly. The physical assignment of PDE6B to Chr 6q33-qter orientates the linkage map on sheep Chr 6 with PDE6B near the telomere and OarCP125 towards the centromere. The order and genetic distances between loci are similar for the sheep Chr 6 and cattle Chr 6 maps, except for the position of the casein genes. The sheep Chr 6 linkage map is also comparable to portions of human Chr 4, mouse Chrs 5 and 3, and pig Chr 8. The synteny between sheep Chr 6 and human Chr 4 has been extended from PDE6B (4p16.3) to epidermal growth factor (EGF, 4q25-q27). However, a region from platelet-derived growth factor receptor alpha polypeptide (PDGFRA) to bone morphogenetic protein 3 (BMP3), which spans 19 cM on sheep Chr 6, appears to be inverted with respect to the human and mouse loci. Other differences in the gene order between sheep, pig, and mouse suggest more complex rearrangements.

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Section: Rflp Analysismentioning

confidence: 99%

The linkage map of sheep Chromosome 6 compared with orthologous regions in other species

et al. 1996

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“…The other fragment will probably have run off the gel. The enzymes most likely to give an RFLP marker in ruminants are MspI and TaqI (Montgomery et al, 1995). The two major disadvantages of these markers are that they are technically demanding and require large quantities of DNA (approximately 5 µg per lane, compared with 1 ng for any PCR-based test).…”

Section: Restriction Fragment Length Polymorphisms (Rflps)mentioning

confidence: 99%