Sheep and Goat Pox

Gelaye, Esayas; Lamien, Charles Euloge

doi:10.1007/978-3-030-25385-1_14

Cited by 7 publications

(5 citation statements)

References 60 publications

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“…Approximately 10-12 specific pathogen-free production (SPF) of embryonate chicken egg (ECE) were purchased from Specific pathogen-free production (SPF) Kaom-Oshan Company, El-Fayoum Egypt. The Chorio-Allantoic Membrane (CAM) protocol for inoculation of the virus for virus isolation in CAM, described in (Gelaye and Lamien, 2019) by using the artificial air sac route. Briefly-Embryos of 10 to 12 days were candled for embryos viability.…”

Section: Virus Propagated In Embryonated Chicken Eggsmentioning

confidence: 99%

“…Several suitable molecular assays have been developed for the detection of CaPVs (Zeedan et al, 2014;Abdallah et al, 2018). Most of DOI: https://dx.doi.org/10.36380/scil.2020.wvj11 these tests were restricted to detect only one viral species (Tassew et al, 2018;Gelaye and Lamien, 2019). RPO 30 gene-based PCR depended on the presence of RPO30 gene encoding the 30 kilo Dalton (kDa) RNA polymerase subunit in Ca PVs (Assefa, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Rapid Detection and Differentiation between Sheep Pox and Goat Pox Viruses by Real-Time qPCR and Conventional PCR in Sheep and Goat in Egypt.

Zeedan¹,

Mahmoud²,

Abdalhamed³

et al. 2020

jwpr

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Capri Pox Virus (Ca PV) is the causative agent of important diseases in sheep and goat with severe socioeconomic impact. Sheep Poxvirus (SPPV), Goat Poxvirus (GTPV) and Lumpy Skin Disease Virus (LSDV) are three members of the Capripox virus genus of Poxviridae family, which infect sheep, goats, and cattle, respectively. A rapid diagnostic assay for Ca PV by using conventional PCR RNA polymerase gene RP030 and real-time qPCR would be useful for disease surveillance, detection and differentiation of Ca PV in clinical and subclinical samples for management and treatments of outbreaks. The present study aimed to detect and identify Ca PV (SPPV and GTPV) in natural, infected scabs biopsy samples, which were collected from sheep and goats in different governorates in 2017 during outbreaks in Egypt using the conventional PCR RNA polymerase gene RP030 gene based and Real-Time qPCR fluorescent based. We collected eighty scabs from clinically affected animals (54 sheep and 26 goat) that were vaccinated in Chorio-Allantoic-Membranes (CAM) from 10-days-old embryonated-chicken eggs. The positive CAM showed pock lesions, which were observed with a thickening of the membrane after 2-3 passages post samples inoculation, and harvested positive CAMs, which were determined by Agar Gel Precipitation Test (AGPT) , Counter Immune Electrophoresis (CIE), and conventional PCR and real time qPCR were examined for the presences of Ca PVs. DNA extraction from clinical samples and positive CAM with pox lesions using DNA slandered references extraction kits compared to novel modification method (Microwave extraction). The PCR based RPO30 gene and the real-time qPCR showed 15 positive with percentage 27.77% in 54 sheep and 3 positive with percentage 12.5% in 26 goats. Although, AGPT and CIE gave lower result than molecular methods, they gave 11 and 13 positive samples from 54 sheep and in goats were 1 and 2 from 26 scab biopsy samples respectively, however they are useful for early confirmation of positive Ca PVs in low-income countries. PCR based RNA polymerase gene RP030 gene and real-time-PCR considered sensitive, rapid, and reliable methods for differentiating SPPV and GTPV from AGPT and CIE in CAM or in clinical samples without further isolation and propagation in embryonated-chicken eggs. The novel microwave method used to isolate high quality of DNA extracted from infected skin biopsy with SPPV and GPPV with no further purification steps required. It was done in 3 minutes only. The results of the current study confirmed that the suitability of the PCR-based RNA polymerase gene RP030 gene is suitable for differentiating between SPPV and GTPV; in one PCR run; without any post-processing steps.

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Section: Virus Propagated In Embryonated Chicken Eggsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid Detection and Differentiation between Sheep Pox and Goat Pox Viruses by Real-Time qPCR and Conventional PCR in Sheep and Goat in Egypt.

Zeedan¹,

Mahmoud²,

Abdalhamed³

et al. 2020

jwpr

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“…SPP and GTP are endemic in Asia, Africa (except for southern Africa), the Middle East, and Turkey [10,11]. In addition, several sporadic SPP incursions have occurred in Greece and some Eastern European countries since 2013 [10].…”

Section: Introductionmentioning

confidence: 99%

Harnessing Attenuation-Related Mutations of Viral Genomes: Development of a Serological Assay to Differentiate between Capripoxvirus-Infected and -Vaccinated Animals

Berguido,

Chibssa,

Loitsch

et al. 2023

Viruses

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Sheeppox, goatpox, and lumpy skin disease caused by the sheeppox virus (SPPV), goatpox virus (GTPV), and lumpy skin disease virus (LSDV), respectively, are diseases that affect millions of ruminants and many low-income households in endemic countries, leading to great economic losses for the ruminant industry. The three viruses are members of the Capripoxvirus genus of the Poxviridae family. Live attenuated vaccines remain the only efficient means for controlling capripox diseases. However, serological tools have not been available to differentiate infected from vaccinated animals (DIVA), though crucial for proper disease surveillance, control, and eradication efforts. We analysed the sequences of variola virus B22R homologue gene for SPPV, GTPV, and LSDV and observed significant differences between field and vaccine strains in all three capripoxvirus species, resulting in the truncation and absence of the B22R protein in major vaccines within each of the viral species. We selected and expressed a protein fragment present in wildtype viruses but absent in selected vaccine strains of all three species, taking advantage of these alterations in the B22R gene. An indirect ELISA (iELISA) developed using this protein fragment was evaluated on well-characterized sera from vaccinated, naturally and experimentally infected, and negative cattle and sheep. The developed wildtype-specific capripox DIVA iELISA showed >99% sensitivity and specificity for serum collected from animals infected with the wildtype virus. To the best of our knowledge, this is the first wildtype-specific, DIVA-capable iELISA for poxvirus diseases exploiting changes in nucleotide sequence alterations in vaccine strains.

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“…Sheeppox and goatpox (SGP) are transboundary small ruminant diseases, caused by sheeppox virus and goatpox virus, of the Capripoxvirus genus, family Poxviridae ( Bhanuprakash et al, 2012 ). SGP is considered endemic in Nigeria ( Gelaye and Lamien, 2019 ). Virus transmission primarily occurs through direct contact or aerosol inhalation ( Bhanuprakash et al, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Financial impact of sheeppox and goatpox and estimated profitability of vaccination for subsistence farmers in selected northern states of Nigeria

Rawlins

Limon

Adedeji

et al. 2022

Preventive Veterinary Medicine

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Sheep and Goat Pox

Cited by 7 publications

References 60 publications

Rapid Detection and Differentiation between Sheep Pox and Goat Pox Viruses by Real-Time qPCR and Conventional PCR in Sheep and Goat in Egypt.

Rapid Detection and Differentiation between Sheep Pox and Goat Pox Viruses by Real-Time qPCR and Conventional PCR in Sheep and Goat in Egypt.

Harnessing Attenuation-Related Mutations of Viral Genomes: Development of a Serological Assay to Differentiate between Capripoxvirus-Infected and -Vaccinated Animals

Financial impact of sheeppox and goatpox and estimated profitability of vaccination for subsistence farmers in selected northern states of Nigeria

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