Shedding Structured Light on Molecular Immunity: The Past, Present and Future of Immune Cell Super Resolution Microscopy

Johanson, Timothy M.; Keenan, Christine R.; Allan, Rhys S.

doi:10.3389/fimmu.2021.754200

Cited by 3 publications

(3 citation statements)

References 106 publications

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“…Thus, we propose that under normal conditions this activation‐induced enhancer assists in regulating this critical and dramatic increase in Myc expression. The development and application of new super‐resolution live imaging technologies that allow concurrent visualization of multiple regions of DNA and associated factors at single molecule resolution, 36 , 37 will likely soon enable the visualization and dissection of not only this function, but the discussed genome architecture of the enhancer deleted Myc regulatory landscape and definition of the factors regulating redundancy.…”

Section: Discussionmentioning

confidence: 99%

Survey of activation‐induced genome architecture reveals a novel enhancer of Myc

et al. 2023

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The transcription factor Myc is critically important in driving cell proliferation, a function that is frequently dysregulated in cancer. To avoid this dysregulation Myc is tightly controlled by numerous layers of regulation. One such layer is the use of distal regulatory enhancers to drive Myc expression. Here, using chromosome conformation capture to examine B cells of the immune system in the first hours after their activation, we reveal a previously unidentified enhancer of Myc. The interactivity of this enhancer coincides with a dramatic, but discrete, spike in Myc expression 3 h post-activation. However, genetic deletion of this region, has little impact on Myc expression, Myc protein level or in vitro and in vivo cell proliferation. Examination of the enhancer deleted regulatory landscape suggests that enhancer redundancy likely sustains Myc expression. This work highlights not only the importance of temporally examining enhancers, but also the complexity and dynamics of the regulation of critical genes such as Myc.

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Section: Discussionmentioning

confidence: 99%

Survey of activation‐induced genome architecture reveals a novel enhancer of Myc

et al. 2023

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“…Thus, we propose that under normal conditions this activation-induced enhancer assists in regulating this critical and dramatic increase in Myc expression. The development and application of new super-resolution live imaging technologies that allow concurrent visualisation of multiple regions of DNA and associated factors at single molecule resolution 35,36 , will likely soon enable the visualisation and dissection of not only this function, but the discussed genome architecture of the enhancer deleted Myc regulatory landscape and definition of the factors regulating redundancy.…”

Section: Discussionmentioning

confidence: 99%

Survey of activation-induced genome architecture reveals a novel enhancer of Myc

Chan¹,

Coughlan²,

Ruhle³

et al. 2022

Preprint

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The transcription factor Myc is critically important in driving cell proliferation, a function that is frequently dysregulated in cancer. To avoid this dysregulation Myc is tightly controlled by numerous layers of regulation. One such layer is the use of distal regulatory enhancers to drive Myc expression. Here, using chromosome conformation capture to examine B cells of the immune system in the first hours after their activation, we reveal a previously unidentified enhancer of myc. The interactivity of this enhancer coincides with a dramatic, but discrete, spike in Myc expression 3 hours post-activation. However, genetic deletion of this region, has little impact on Myc expression, Myc protein level or in vitro and in vivo cell proliferation. Examination of the enhancer deleted regulatory landscape suggests that enhancer redundancy likely sustains Myc expression. This work highlights not only the importance of temporally examining enhancers, but also the complexity and dynamics of the regulation of critical genes such as Myc.

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“…In brief, these principles include illumination modulation (e.g., STED [ 4 , 5 ], SIM [ 6 – 9 ]), single-molecule localization (e.g., SMLM [ 10 – 13 ]), fluctuation analysis (e.g., SOFI [ 14 ], SRRF [ 15 , 16 ]), and physical sample manipulation (e.g., ExM [ 17 , 18 ]). These methodologies exhibit unique characteristics that have facilitated their extensive application spanning basic and translational disciplines, such as cell biology [ 19 ], immunology [ 20 ], neuroscience [ 21 ], and pathology [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

hydroSIM: super-resolution speckle illumination microscopy with a hydrogel diffuser

Gao,

Han,

Hua

et al. 2024

Biomed. Opt. Express

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Super-resolution microscopy has emerged as an indispensable methodology for probing the intricacies of cellular biology. Structured illumination microscopy (SIM), in particular, offers an advantageous balance of spatial and temporal resolution, allowing for visualizing cellular processes with minimal disruption to biological specimens. However, the broader adoption of SIM remains hampered by the complexity of instrumentation and alignment. Here, we introduce speckle-illumination super-resolution microscopy using hydrogel diffusers (hydroSIM). The study utilizes the high scattering and optical transmissive properties of hydrogel materials and realizes a remarkably simplified approach to plug-in super-resolution imaging via a common epi-fluorescence platform. We demonstrate the hydroSIM system using various phantom and biological samples, and the results exhibited effective 3D resolution doubling, optical sectioning, and high contrast. We foresee hydroSIM, a cost-effective, biocompatible, and user-accessible super-resolution methodology, to significantly advance a wide range of biomedical imaging and applications.

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Shedding Structured Light on Molecular Immunity: The Past, Present and Future of Immune Cell Super Resolution Microscopy

Cited by 3 publications

References 106 publications

Survey of activation‐induced genome architecture reveals a novel enhancer of Myc

Survey of activation‐induced genome architecture reveals a novel enhancer of Myc

Survey of activation-induced genome architecture reveals a novel enhancer of Myc

hydroSIM: super-resolution speckle illumination microscopy with a hydrogel diffuser

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