Shedding of Neospora caninum oocysts by dogs fed tissues from naturally infected water buffaloes (Bubalus bubalis) from Brazil

Rodrigues, Aline Aparecida Resende; Gennari, Solange Maria; Aguiar, Daniel Moura de; Sreekumar, C.; Hill, Dolores E.; Miska, Katarzyna B.; Vianna, M.C.B.; Dubey, J. P.

doi:10.1016/j.vetpar.2004.07.007

Cited by 72 publications

(71 citation statements)

References 35 publications

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“…High prevalence values of anti-N. caninum antibodies have been observed in buffalo worldwide (Dubey et al 1998, Guarino et al 2000, Meenakshi Sandhu et al 2007). The parasite has also been isolated from naturally infected water buffalo (Rodrigues et al 2004), demonstrating that this animal species is an important natural intermediate host for N. caninum. Recently, experimental vertical transmission and abortion by this pathogen have been described in pregnant water buffalo (Konrad et al 2012).…”

Section: Introductionmentioning

confidence: 96%

PCR detection of Neospora caninum in water buffalo foetal tissues

Auriemma

Lucibelli

Borriello

et al. 2014

Acta Parasitologica

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The seroprevalence of Neospora caninum was surveyed by an ELISA kit on two water buffalo herds of Southern Italy. Seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. Tissue samples collected from three aborted fetuses from the same herds were investigated for N. caninum presence by PCR assays targeting the 18S and the Nc5 DNA sequences, respectively. Both methods have shown the presence of N. caninum DNA in heart and brain. Sequencing of the Nc5 genomic DNA confirmed the presence of N. caninum in the samples; phylogenetic analysis of the obtained sequences showed high homology among the Neospora recovered from different samples. The present study suggests an important role of N. caninum as a possible abortive agent for water buffaloes.

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Section: Introductionmentioning

confidence: 96%

PCR detection of Neospora caninum in water buffalo foetal tissues

Auriemma

Lucibelli

Borriello

et al. 2014

Acta Parasitologica

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“…N. caninum is now a matter of international concern as it has been recognised as a major cause of infectious abortion in the main cattle-producing countries (Dubey 2003a). Cattle and other ungulates such as sheep, goats, horses, white-tailed deer, rhinoceros, South American camelids and water buffaloes may act as natural intermediate hosts (Dubey 2003b, Chávez-Velásquez et al 2004, Rodrigues et al 2004. N. caninum infection has been detected in other animal species such as cats, opossums, foxes and other wild canids (Dubey 2003b, Moore 2005).…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of bovine neosporosis: Recent advances and perspectives

Ortega‐Mora

Fernández-García

Gómez-Bautista

2006

Acta Parasitologica

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Neospora caninum is considered a major cause of abortion in cattle. Appropriate techniques for diagnosis of bovine neosporosis, both in vivo and in aborted foetuses, have been developed in the last ten years and some of them are commercially available. For diagnosis in live animals, detection of antibodies in serum or milk has been shown to be the best option both at the herd and the individual level. These techniques are excellent tools to examine N. caninum-associated abortion problems and to adopt some basic herd-control measures. Concerning foetal diagnosis, detection of compatible lesions by histological examination and parasites by PCR in brain (as well as heart and liver) are the best choices. Diagnostic criteria to distinguish foetal infection and Neospora-associated abortion are based not only on the demonstration of the parasite in the foetus but also on the extent and severity of the lesions in the foetus, foetal age and the assessment of neosporosis at the herd level. In the near future, new tools to diagnose infection should help to detect animals with parasite reactivation by testing the immune response to stage-specific antigens and lead to the development of molecular typing methods to characterise different parasite isolates. Finally, uniform diagnostic procedures need to be established between laboratories and countries in order to standardise result interpretation. The role of National or Regional Reference Laboratories is essential in countries or regions where control programmes for the disease are being developed.

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“…O N. caninum já foi isolado de bubalinos (RODRIGUES et al, 2004) e, atualmente, investiga-se a importância dessa espécie na epidemiologia da enfermidade, uma vez que muitas vezes os bubalinos são criados juntamente com os bovinos (GENNARI, 2004). Nesse sentido, já foi demonstrado que bovinos podem ser infectados com isolados de N. caninum de bubalinos (RODRIGUES et al, 2004). Estudos sorológicos nessa espécie demonstraram índices de 36,5% de soropositivos na Bahia (GONDIM et al, 1999), de 56% (SOUZA et al, 2001) e de 64% (FUJJI et al, 2001) em SP, de 70,9% no Pará (GENNARI et al, 2005.…”

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Anticorpos anti-Neospora caninum em bovinos, ovinos e bubalinos no Estado do Rio Grande do Sul

2006

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RESUMO A infecção peloO Neospora caninum (N. caninum) é um protozoário coccídio que tem sido considerado a principal causa de abortos em bovinos (ANDERSON et al., 1991). O agente foi descrito e caracterizado inicialmente em cães nos Estados Unidos (DUBEY et al., 1998) e, desde a sua descrição inicial, tem sido diagnosticado em várias espécies, inclusive em bovinos (DUBEY & LINDSAY, 1996). A enfermidade associada à infecção por esse parasita é denominada de neosporose.O ciclo biológico do Neospora caninum foi primeiramente descrito por MCALLISTER et al. (1998) e envolve a presença de hospedeiros definitivos e intermediários. O ciclo do protozoário envolve três estágios infecciosos: os bradizoítos, os taquizoítos e os esporozoítos. Os taquizoítos e os bradizoítos são estágios intracelulares encontrados no hospedeiro intermediário, enquanto que os esporozoítos se desenvolvem dentro dos oocistos no processo de esporulação (DUBEY, 2003

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Shedding of Neospora caninum oocysts by dogs fed tissues from naturally infected water buffaloes (Bubalus bubalis) from Brazil

Cited by 72 publications

References 35 publications

PCR detection of Neospora caninum in water buffalo foetal tissues

PCR detection of Neospora caninum in water buffalo foetal tissues

Diagnosis of bovine neosporosis: Recent advances and perspectives

Anticorpos anti-Neospora caninum em bovinos, ovinos e bubalinos no Estado do Rio Grande do Sul

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