Shedding of microparticles by myofibroblasts as mediator of cellular cross‐talk during normal wound healing

Moulin, Véronique J.; Mayrand, Dominique; Messier, Hugo; Martinez, Maria Carmen; Lopez-Vallé, Carlos A.; Genest, Hervé

doi:10.1002/jcp.22268

Cited by 31 publications

(51 citation statements)

References 32 publications

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“…During these phases, a series of precise biological events occur including: rapid vascular spasms, platelet plug formation and coagulation (collectively known as haemostasis); inflammation; cell migration into the wound site; cell proliferation and differentiation to form granulation tissue; angiogenesis; and extracellular-matrix reorganisation [97]. Evidence for the involvement of EVs in wound healing has been described for coagulation, cell proliferation, cell migration and angiogenesis and summarised in Table 1 [17,18,19,21]. …”

Section: The Role Of Evs In Cutaneous Wound Healingmentioning

confidence: 99%

“…Cell proliferation, a pivotal cellular process underpinning wound healing, has been shown to be regulated by EVs derived from a plethora of cell types, such as human mesenchymal stem cells (MSCs) [21,100,101], fibroblasts [18], murine embryonic stem cells [102], and human endothelial progenitor cells [103]. The regulation of cell proliferation by EVs occurred through the binding to or internalisation of EVs into recipient cells and delivery of EV content such as RNAs [104,105].…”

Section: The Role Of Evs In Cutaneous Wound Healingmentioning

confidence: 99%

“…Preliminary studies have shown that EVs may be involved in wound healing through the control of a number of cellular processes [17,18,19,20,21]. Therefore, this review will summarise the role of EVs derived from predominant cells involved in the wound healing process.…”

Section: Introductionmentioning

confidence: 99%

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Association of Extracellular Membrane Vesicles with Cutaneous Wound Healing

Than

Guanzon

Leavesley

et al. 2017

IJMS

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Extracellular vesicles (EVs) are membrane-enclosed vesicles that are released into the extracellular environment by various cell types, which can be classified as apoptotic bodies, microvesicles and exosomes. EVs have been shown to carry DNA, small RNAs, proteins and membrane lipids which are derived from the parental cells. Recently, several studies have demonstrated that EVs can regulate many biological processes, such as cancer progression, the immune response, cell proliferation, cell migration and blood vessel tube formation. This regulation is achieved through the release and transport of EVs and the transfer of their parental cell-derived molecular cargo to recipient cells. This thereby influences various physiological and sometimes pathological functions within the target cells. While intensive investigation of EVs has focused on pathological processes, the involvement of EVs in normal wound healing is less clear; however, recent preliminarily investigations have produced some initial insights. This review will provide an overview of EVs and discuss the current literature regarding the role of EVs in wound healing, especially, their influence on coagulation, cell proliferation, migration, angiogenesis, collagen production and extracellular matrix remodelling.

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Section: The Role Of Evs In Cutaneous Wound Healingmentioning

confidence: 99%

Section: The Role Of Evs In Cutaneous Wound Healingmentioning

confidence: 99%

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Association of Extracellular Membrane Vesicles with Cutaneous Wound Healing

Than

Guanzon

Leavesley

et al. 2017

IJMS

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“…Wound myofibroblasts (MyoFb), were obtained from implants inserted subcutaneously in the arms of volunteers as described previously by Germain et al [15]. Dermal fibroblasts (Fb) and human dermal microvascular endothelial cells (HDMVEC) were isolated from skin biopsies obtained during myofibroblast implant surgeries [16]. All specimens were obtained after the informed consent of the donor and according to the principles expressed in the Helsinki Declaration.…”

Section: Cell Culturementioning

confidence: 99%

Angiogenic properties of myofibroblasts isolated from normal human skin wounds

et al. 2012

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During wound healing, angiogenesis plays a crucial role in inducing adequate perfusion of the new tissue, thereby allowing its survival. This angiogenic process contributes to the formation of granulation tissue, alongside myofibroblasts. Myofibroblasts are cells specialized in wound contraction and synthesis of new extracellular matrix. Fibroblasts, considered by some to be at the origin of myofibroblasts, have already been shown to promote neovascularization. Thus, we hypothesized that myofibroblasts play a key role during angiogenic development in wound healing. We isolated myofibroblasts from normal human skin wounds and dermal microvascular endothelial cells (HDMVEC) and fibroblasts from skin. Using an in vitro fibrin-based model, we compared the proangiogenic activity of wound myofibroblasts to that of fibroblasts in the presence of HDMVEC. By immunostaining with collagen IV antibodies, we observed the formation of a capillary network significantly more developed when HDMVEC were cultured with myofibroblasts compared to the network formed in the presence of fibroblasts. The differences between these cell types did not result from a differential secretion of Vascular Endothelial Growth Factor or basic Fibroblast Growth Factor. However, in the presence of myofibroblasts, a significant decrease in matrix metalloproteinase activity was observed. This finding was correlated with a significant increase in Tissue Inhibitor of MetalloProteinase (TIMP)-1 and TIMP-3. Furthermore, inhibition of TIMP-1 secretion using shRNA significantly decreased myofibroblasts induced angiogenesis. These results led to the hypothesis that normal wound myofibroblasts contribute to the vascular network development during wound healing. Our data emphasize the critical role of wound myofibroblasts during healing.

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“…These MP could transfer bioactive molecules such as growth factors from myofibroblasts to other cells present into the wound and participate in the healing process. 34 …”

Section: Mp Production Is Influenced By Underlying Conditionsmentioning

confidence: 99%

Development, Cell Differentiation, Angiogenesis—Microparticles and Their Roles in Angiogenesis

Shai

Varon

2011

ATVB

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Particular attention had been paid to this relatively novel method of cell communication. Cells of various types release small particles into body fluids, and these particles apparently transfer messages to other cells and tissues. The term suggested for this signaling method was "rececrine," which refers to the "secretion" of an active cellular receptor that can be incorporated to the particle cargo and transferred to a target cell, where it can function and signal in an ectopic manner. 1 MP are released from the plasma membrane of cells by outward budding of membrane vesicles, following disruption of the natural asymmetrical distribution of membrane phospholipids, under conditions of cell activation, mechanical stress, and apoptosis. 2 Studies suggest that MP shedding is a highly regulated process that occurs in a spectrum of cell types, and MP have been widely detected in various biological fluids, including peripheral blood and urine.MP contain proteins derived from the parent cell, although their protein and membrane content are not identical to that of the original cell. In recent years, it has become clear that they have important biological functions, including horizontal transfer of bioactive molecules, such as receptors, integrins, 3 growth factors, RNAs, 4 and microRNAs. The function of MP is dependent on the cell type from which they originate and their content. Platelet-derived microparticles (PMP) participate in blood coagulation, whereas MP secreted by skeletal cells initiate bone mineralization, and MP secreted by normal endothelial cells have been implicated in angiogenesis. 5 It was shown that the effect of endothelial-derived MP on angiogenesis is dose-dependent and that whereas endothelialderived MP promote angiogenesis in vitro in low concentrations, high concentrations may suppress angiogenesis. 6,7 Tumor cell-derived MP have been shown to facilitate extracellular matrix invasion and evasion of the immune response. 8 It is important to discriminate MP from exosomes, which originate from intracellular multivesicular bodies. Exosomes are smaller in size (Ͻ0.1 m) and are morphologically distinct. Muralidharan-Chari et al 9 showed that MP appear to be rather heterogeneous in size, ranging from 100 nm to 1 m in diameter, whereas exosomes are more uniform and range from 50 to 80 nm in diameter. MP also pellet at less than 100 000 g, compared with exosomes, which need at least Angiogenesis is a tightly regulated process that involves endothelial cell survival, proliferation, migration, differentiation, and morphological changes, such as tube formation. It is a major process in many pathological conditions, such as tumor growth, diabetic retinopathy, and inflammation, as well as in embryonic development and wound healing. 10 In this review, we focus on MP involvement in various pathological conditions, showing their potential use as disease markers, and we present some data emphasizing their potential application in tissue regeneration. MP Affect Endothelial CellsMost of the research regarding MP h...

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Shedding of microparticles by myofibroblasts as mediator of cellular cross‐talk during normal wound healing

Cited by 31 publications

References 32 publications

Association of Extracellular Membrane Vesicles with Cutaneous Wound Healing

Association of Extracellular Membrane Vesicles with Cutaneous Wound Healing

Angiogenic properties of myofibroblasts isolated from normal human skin wounds

Development, Cell Differentiation, Angiogenesis—Microparticles and Their Roles in Angiogenesis

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