Shedding light on response of Triticum aestivum cv. Kharchia Local roots to long-term salinity stress through transcriptome profiling

Mahajan, Mahesh; Goyal, Etika; Singh, Amit Kumar; Gaikwad, Kishor; Kumar, Kanika

doi:10.1007/s10725-019-00565-4

Cited by 16 publications

(5 citation statements)

References 74 publications

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“…Landraces adapted to these regions are mostly tolerant to abiotic stresses viz. drought, heat, and salinity, e.g., genotype KHARCHIA LOCAL (origin: Rajasthan, India) is adapted to long-term salinity stress (Mahajan et al, 2017(Mahajan et al, , 2020. We hypothesize that landraces adapted to harsh environments, such as abiotic stresses and low-input conditions give an eluded appearance of diseases.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Studies in Diverse Spring Wheat Panel for Stripe, Stem, and Leaf Rust Resistance

Kumar

Chhokar

et al. 2020

Front. Plant Sci.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Studies in Diverse Spring Wheat Panel for Stripe, Stem, and Leaf Rust Resistance

Kumar

Chhokar

et al. 2020

Front. Plant Sci.

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“…They showed that genes involved in ROS homeostasis, ion transport, signal transduction, ABA biosynthesis and osmoregulation up-regulated in response to salt stress. They also indicated that genes coding for expansin, dehydrins, xyloglucan endotransglucosylase and peroxidases, engaged in root growth improvement, up-regulated under salt stress [ 20 ]. Despite the valuable insight discovered by recent researches about the cellular and molecular mechanisms engaged in salinity stress response and tolerance in bread wheat, many aspects are still uncovered.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of bread wheat leaves in response to salt stress

et al. 2021

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Salinity is one of the main abiotic stresses limiting crop productivity. In the current study, the transcriptome of wheat leaves in an Iranian salt-tolerant cultivar (Arg) was investigated in response to salinity stress to identify salinity stress-responsive genes and mechanisms. More than 114 million reads were generated from leaf tissues by the Illumina HiSeq 2500 platform. An amount of 81.9% to 85.7% of reads could be mapped to the wheat reference genome for different samples. The data analysis led to the identification of 98819 genes, including 26700 novel transcripts. A total of 4290 differentially expressed genes (DEGs) were recognized, comprising 2346 up-regulated genes and 1944 down-regulated genes. Clustering of the DEGs utilizing Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that transcripts associated with phenylpropanoid biosynthesis, transporters, transcription factors, hormone signal transduction, glycosyltransferases, exosome, and MAPK signaling might be involved in salt tolerance. The expression patterns of nine DEGs were investigated by quantitative real-time PCR in Arg and Moghan3 as the salt-tolerant and susceptible cultivars, respectively. The obtained results were consistent with changes in transcript abundance found by RNA-sequencing in the tolerant cultivar. The results presented here could be utilized for salt tolerance enhancement in wheat through genetic engineering or molecular breeding.

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“…The summary of the sequence assembly statistics is given in Table 1. The average GC content of finger millet was 48.92%, which is in the range of GC levels of monocot coding sequences (Goyal et al 2016 a, b; Mahajan et al 2017, 2020). A total of 13,642, 27,910, 17,031, and 23,659 ORFs were detected in KJNS-C, KJNS-T, PES-C, and PES-T, respectively (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Comparative transcriptome analysis in contrasting finger millet (Eleusine coracana(L.) Gaertn) genotypes for heat stress

Goyal

Singh

Mahajan

et al. 2023

Preprint

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Eleusine coracana (L.) Gaertn is a crucial C4 species renowned for its stress robustness and nutritional significance. Because of its adaptability traits, finger millet (ragi) is a storehouse of critical genomic resources for crop improvement. However, more knowledge about this crop's molecular responses to heat stress must be gained. Hence, in the present study, we generated RNA seq data from the leaf tissue of the finger millet to observe the physiological changes and gene expression study in heat-sensitive (KJNS-46) and heat-tolerant (PES-110) genotypes of Ragi in response to high temperatures. On average, each sample generated about 24 million reads. Nearly 684 transcripts were differentially expressed (DEGs) between the heat-stressed samples of both genotypes. Pathway analysis and functional annotation analysis revealed the activation of various genes involved in response to stress, precisely heat, oxidation-reduction process, water deprivation, heat shock protein and transcription factors, calcium, and kinase signaling. The basal regulatory genes, such as bZIP, were involved in response to heat stress, indicating that heat stress activates genes related to basal regulatory processes or housekeeping. A substantial percentage of the DEGs belonged to proteins of unknown functions (PUFs), i.e., uncharacterized. The expression pattern of a few selected DEGs genes was analyzed in both genotypes by quantitative RT-PCR. The present study found some candidate genes and pathways that may confer tolerance to heat stress in ragi. These results will provide valuable information to improve heat tolerance in heat-susceptible agronomically important varieties of ragi and other crop plants.

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Shedding light on response of Triticum aestivum cv. Kharchia Local roots to long-term salinity stress through transcriptome profiling

Cited by 16 publications

References 74 publications

Genome-Wide Association Studies in Diverse Spring Wheat Panel for Stripe, Stem, and Leaf Rust Resistance

Genome-Wide Association Studies in Diverse Spring Wheat Panel for Stripe, Stem, and Leaf Rust Resistance

Transcriptome analysis of bread wheat leaves in response to salt stress

Comparative transcriptome analysis in contrasting finger millet (Eleusine coracana(L.) Gaertn) genotypes for heat stress

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