Shear Stress Regulates Adhesion and Rolling of CD44+ Leukemic and Hematopoietic Progenitor Cells on Hyaluronan

Christophis, Christof; Taubert, Isabel; Meseck, Georg R.; Schubert, Mario; Grunze, M.; Ho, Anthony D.; Rosenhahn, Axel

doi:10.1016/j.bpj.2011.05.045

Cited by 53 publications

(59 citation statements)

References 61 publications

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“…It should be mentioned that the rolling behaviors are not identical between the cells expressing CD44 and the beads displaying the HABD. For instance, the stabilization of the rolling (e.g., increase of adhesion numbers or decrease of rolling velocities) at high shear stress was observed only for the experiments using cells (21,23). Those differences may be ascribed to the cellular features, such as the formation of microvilli, slings (26), and cell flattening, which also contribute to stable rolling (27).…”

Section: Discussionmentioning

confidence: 98%

“…Recently, it was reported that the rolling of CD44-expressing cells is enhanced at the higher shear stress (23), raising the possibility that CD44 possesses some mechanochemical specializations to resist higher tensile force. Considering the fact that the C terminus of CD44 HABD is connected to the plasma membrane, the force applied from the C terminus of HABD would induce the allosteric transition from the O to the PD state, thereby providing the resistance to the applied force.…”

Section: E7304-e7305mentioning

confidence: 99%

See 1 more Smart Citation

Mechanical force effect on the two-state equilibrium of the hyaluronan-binding domain of CD44 in cell rolling

Suzuki

Ogino

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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The authors note that Fig. 4 and Fig. S4 appeared incorrectly. The corrected figures and their legends appear below. The SI has been corrected online.The authors also note that on page 1 of the supporting information, right column, second full paragraph, lines 5-13, "The starting structure was solvated in a 62 × 129 × 69-Å TIP3 water box (6) with 54 chloride ions to neutralize the system, resulting in 53,872 atoms. The system was minimized for three consecutive 500-conjugate gradient steps, during which the protein was first held fixed; next, only the heavy atoms of the protein were held fixed, and finally, all atoms were allowed to move. After the energy minimizations, the system was heated gradually from 0 K to 310 K over 52 ps, and the temperature was maintained using Langevin dynamics" should instead appear as "The starting structure was solvated in a 68 × 116 × 79-Å TIP3 water box (6) with 53 chloride ions to neutralize the system, resulting in 59,606 atoms. The system was minimized for two consecutive 500-conjugate gradient steps, during which the protein was first held fixed; next, only the heavy atoms of the protein were held fixed. After the energy minimizations, the system was heated gradually from 0 K to 310 K over 9.3 ps, and the temperature was maintained using Langevin dynamics. Subsequently, the system was equilibrated by 4 rounds, first the heavy atoms were held fixed, then only backbone atoms, then only Cα atoms, and finally all atoms were allowed to move." These errors do not affect the conclusions of the article.A B C Fig. 4. Steered molecular dynamics (SMD) simulation of CD44 HABD by pulling force. (A) Snapshots of the HABD-HA8 complex at 0 ns (Left) and 3.5 ns (Right) during the SMD simulation. In the SMD simulation, the Cα carbon of the C-terminal residue Ile173 (gray sphere) was pulled at 10 Å/ns in the indicated direction (arrow), whereas the C2 atom of the N-acetylglucosamine residue (green sphere) was kept fixed. In the snapshot after 3.5 ns in the SMD simulation, the C-terminal mechanosensitive latch was completely separated from the α1 helix. (B) The time course of the hydrogen bond donor-acceptor distances between HA NAG1177 and I100 (red) and E52 and Y166 (blue). (C) Schematic depiction of the mechanosensitive latch in the force-induced conformational change of CD44 HABD.

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Section: Discussionmentioning

confidence: 98%

Section: E7304-e7305mentioning

confidence: 99%

Mechanical force effect on the two-state equilibrium of the hyaluronan-binding domain of CD44 in cell rolling

Suzuki

Ogino

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…It has been reported that the strength of the biofilms would decrease when the cell viability in the biofilms reduced [52][53][54]. Thus, the potential mechanism for the biofilm inhibition effect in this study is that thermal shock caused disintegration of the bacterial cell membrane and killed the cells in the biofilms.…”

Section: Discussionmentioning

confidence: 83%

Using Thermal Shock to Inhibit Biofilm Formation in the Treated Sewage Source Heat Pump Systems

2017

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Treated sewage source heat pump systems can reuse the waste energy in the treated sewage. However, biofilms in the heat exchangers decrease the system efficiency. This work investigates the feasibility of thermal shock at accessible temperatures in heat exchangers for biofilm inhibition. Bacillus subtilis biofilms were formed on coupons and in a miniaturized plate heat exchanger. Thermal shocks at different temperatures (50-80 • C) for different exposure times (1-60 min) were used to treat the biofilms. The results showed that thermal shock had a significant bactericidal and biofilm inhibition effect, and the effect was enhanced as the temperature and the exposure time increased. Data fitting of the biomass showed that temperature had a more significant influence on the biofilm inhibition effect than exposure time. The results of the heat exchanging experiments showed that high temperature thermal shock could significantly mitigate the heat transfer deterioration caused by the biofilms, indicating that thermal shock could be used as a viable biofilm inhibition approach for heat exchangers.

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“…Furthermore this new assay revealed that cell removal from directed nanostructures depends on the flow direction [23] and that CD44? leukemic cells attach to hyaluronans by a catch bond activated binding [24].…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Assay to Quantify the Adhesion of Marine Bacteria

2012

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For both, environmental and medical applications, the quantification of bacterial adhesion is of major importance to understand and support the development of new materials. For marine applications, the demand is driven by the quest for improved fouling-release coatings. To determine the attachment strength of bacteria to coatings, a microfluidic adhesion assay has been developed which allows probing at which critical wall shear stress bacteria are removed from the surface. Besides the experimental setup and the optimization of the assay, we measured adhesion of the marine bacterium Cobetia marina on a series of differently terminated self-assembled monolayers. The results showed that the adhesion strength of C. marina changes with surface chemistry. The difference in critical shear stress needed to remove bacteria can vary by more than one order of magnitude if a hydrophobic material is compared to an inert chemistry such as polyethylene glycol.

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Shear Stress Regulates Adhesion and Rolling of CD44+ Leukemic and Hematopoietic Progenitor Cells on Hyaluronan

Cited by 53 publications

References 61 publications

Mechanical force effect on the two-state equilibrium of the hyaluronan-binding domain of CD44 in cell rolling

Mechanical force effect on the two-state equilibrium of the hyaluronan-binding domain of CD44 in cell rolling

Using Thermal Shock to Inhibit Biofilm Formation in the Treated Sewage Source Heat Pump Systems

Microfluidic Assay to Quantify the Adhesion of Marine Bacteria

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