Shear-induced platelet aggregation in aspirin-treated patients: Initial experience with the novel PlaCor PRT® device

Rapid evaluation of platelet function using the Multiplate® Analyzer

“…First, it may be used to explain ongoing bleeding and guide treatment and second, it holds the promise of improved periprocedural antithrombotic treatment [10]. Despite the abundance of available point-of-care tests to evaluate platelet function, only a few are designed to deliver immediate answers [16,17], which may be particularly relevant in the setting of acute bleeding. Importantly, there is a need for studies exploring the clinical benefits of rapid platelet function testing [10,18].…”

Characterization of multiple platelet activation pathways in patients with bleeding as a high-throughput screening option: use of 96-well Optimul assay

“…[2][3][4] Several attempts at developing a high-throughput platelet function assay are currently being investigated. These include devices that measure global platelet reactivity in response to shear alone, 5 microfluidic devices with precoated adhesion or activation molecules, [6][7][8] assays that measure calcium flux in platelets by fluorescent imaging, 9 enzyme-linked immunosorbent assay-type assays to capture platelets on agonist-coated surfaces, 10 luminometric assays of platelet secretion in response to various platelet agonists, 11 and flow cytometric counting techniques with platelet immunostaining. 12 Most of these techniques require specialized laboratory instruments (flow cytometers, osmotic pumps, microscopes, imaging devices) or can be expensive to perform (single use precoated cartridges or capillaries, fluorescent antibodies), and not all are sensitive to mild platelet inhibition, 10 making them unsuitable for use in nonspecialized centers.…”

Diagnosis and Management of Acquired von Willebrand Disease in Heart Disease: A Review of the Literature

“…25 Even though Würtz et al 25 found no correlation between the PlaCor PRT® and vWF antigen, no other parameters of vWF activity, in particular those closely related to hemostatic properties, were evaluated in their study. 25 The activity of vWF may be indirectly assessed using POC platelet function analyzers based on impedance aggregometry utilizing ristocetin as a platelet function agonist. 26 These devices are based on the relationship the between impedance and the number of platelets adhering to the two electrodes through which an alternating current is passed.…”

“…In contrast to the PFA-100, the PlaCor PRT® performs function testing using non-anticoagulated whole blood and is based on platelet activation induced solely by shear stress forces, without the use of platelet function agonists. 25 The device has not been validated in a clinical setting to date. 25 Even though Würtz et al 25 found no correlation between the PlaCor PRT® and vWF antigen, no other parameters of vWF activity, in particular those closely related to hemostatic properties, were evaluated in their study.…”

“…25 The device has not been validated in a clinical setting to date. 25 Even though Würtz et al 25 found no correlation between the PlaCor PRT® and vWF antigen, no other parameters of vWF activity, in particular those closely related to hemostatic properties, were evaluated in their study. 25 The activity of vWF may be indirectly assessed using POC platelet function analyzers based on impedance aggregometry utilizing ristocetin as a platelet function agonist.…”