Shared Y chromosome repetitive DNA sequences in stallion and donkey as visualized using whole-genomic comparative hybridization

Gosálvez, Jaime; Crespo, F.; Vega-Pla, J.L.; López‐Fernández, Carmen; Cortés‐Gutiérrez, Elva I.; Devila-Rodriguez, M.I.; Mezzanotte, R.

doi:10.4081/ejh.2010.e2

Cited by 8 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This technique has provided evidence for the presence of polymorphisms in insects where knowledge about the presence of these polymorphisms was low and mainly constrained to variations in C-banded regions. 13 In mammalian species, unexplored polymorphisms were identified in swine, sheep, stallion, and donkey, 14 – 16 and the technique also provided evidence for the presence of highly conserved repetitive DNA sequences on chromosome 9 when human and gorilla chromosomes were compared. 17 Accordingly, W-CGH is a reliable tool for the comparison of the levels of polymorphism of repetitive DNA sequences in the human genome, giving a one-shot experimental hybridization for comparing two different genomes.…”

Section: Discussionmentioning

confidence: 99%

Constitutive heterochromatin polymorphisms in human chromosomes identified by whole comparative genomic hybridization

Mi¹,

Gutiérrez²,

Rm³

et al. 2011

Eur J Histochem

View full text Add to dashboard Cite

Whole comparative genomic hybridization (W-CGH) is a new technique that reveals cryptic differences in highly repetitive DNA sequences, when different genomes are compared using metaphase or interphase chromosomes. W-CGH provides a quick approach to identify differential expansion of these DNA sequences at the single-chromosome level in the whole genome. In this study, we have determined the frequency of constitutive chromatin polymorphisms in the centromeric regions of human chromosomes using a whole-genome in situ cross-hybridization method to compare the whole genome of five different unrelated individuals. Results showed that the pericentromeric constitutive heterochromatin of chromosome 6 exhibited a high incidence of polymorphisms in repetitive DNA families located in pericentromeric regions. The constitutive heterochromatin of chromosomes 5 and 9 was also identified as highly polymorphic. Although further studies are necessary to corroborate and assess the overall incidence of these polymorphisms in human populations, the use of W-CGH could be pertinent and of clinical relevance to assess rapidly, from a chromosomal viewpoint, genome similarities and differences in closely related genomes such as those of relatives, or in more specific situations such as bone marrow transplantation where chimerism is produced in the recipient.

show abstract

Section: Discussionmentioning

confidence: 99%

Constitutive heterochromatin polymorphisms in human chromosomes identified by whole comparative genomic hybridization

Mi¹,

Gutiérrez²,

Rm³

et al. 2011

Eur J Histochem

View full text Add to dashboard Cite

show abstract

“…3 , 5 This fraction of the genome seems to escape the selective pressure actings on the non-repetitive segments, representing good evolutionary marker in studies of species evolution, chromosome structure and function, and in the detection of chromosomal rearrangements, supernumerary and sex chromosomes. 6 – 8 The molecular organization and chromosome location of repetitive DNAs have been analyzed in a large number of fish species. 9 – 13 These studies have demonstrated the enormous potential that the investigation of repetitive DNAs offers in extending our knowledge of karyotype differentiation in fish.…”

Section: Introductionmentioning

confidence: 99%

Identification of two new repetitive elements and chromosomal mapping of repetitive DNA sequences in the fish Gymnothorax unicolor (Anguilliformes: Muraenidae)

et al. 2011

Self Cite

View full text Add to dashboard Cite

Muraenidae is a species-rich family, with relationships among genera and species and taxonomy that have not been completely clarified. Few cytogenetic studies have been conducted on this family, and all of them showed the same diploid chromosome number (2n=42) but with conspicuous karyotypic variation among species. The Mediterranean moray eel Gymnothorax unicolor was previously cytogenetically studied using classical techniques that allowed the characterization of its karyotype structure and the constitutive heterochromatin and argyrophilic nucleolar organizer regions (Ag-NORs) distribution pattern. In the present study, we describe two new repetitive elements (called GuMboI and GuDdeI) obtained from restricted genomic DNA of G. unicolor that were characterized by Southern blot and physically localized by in situ hybridization on metaphase chromosomes. As they are highly repetitive DNA sequences, they map in heterochromatic regions. However, while GuDdeI was localized in the centromeric regions, the GuMboI fraction was distributed on some centromeres and was co-localized with the nucleolus organizer region (NOR). Comparative analysis with other Mediterranean species such as Muraena helena pointed out that these DNA fractions are species-specific and could potentially be used for species discrimination. As a new contribution to the karyotype of this species, we found that the major ribosomal genes are localized on acrocentric chromosome 9 and that the telomeres of each chromosome are composed of a tandem repeat derived from a poly-TTAGGG DNA sequence, as it occurs in most vertebrate species. The results obtained add new information useful in comparative genomics at the chromosomal level and contribute to the cytogenetic knowledge regarding this fish family, which has not been extensively studied.

show abstract

“…Polymorphisms due to the accretion of duplicated genomic segments are common in mammalian genomes, in particular at centromeric and telomeric regions where they are usually silenced and show no effect on the phenotype [12][13][14][15]. In mammalian species, unexplored polymorphisms were identified in swine, sheep, stallions, and donkeys [16][17][18], and the technique also provided evidence for the presence of highly conserved repetitive DNA.…”

Section: Discussionmentioning

confidence: 99%

Digital Agenesia in Martina Franca Donkey Foal: A Case Report

Robbe

Carluccio

Gloria

et al. 2012

Journal of Equine Veterinary Science

View full text Add to dashboard Cite

Shared Y chromosome repetitive DNA sequences in stallion and donkey as visualized using whole-genomic comparative hybridization

Cited by 8 publications

References 27 publications

Constitutive heterochromatin polymorphisms in human chromosomes identified by whole comparative genomic hybridization

Constitutive heterochromatin polymorphisms in human chromosomes identified by whole comparative genomic hybridization

Identification of two new repetitive elements and chromosomal mapping of repetitive DNA sequences in the fish Gymnothorax unicolor (Anguilliformes: Muraenidae)

Digital Agenesia in Martina Franca Donkey Foal: A Case Report

Contact Info

Product

Resources

About