Shared larval rearing environment, sex, female size and genetic diversity shape Ae. albopictus bacterial microbiota

Abstract

The Asian tiger mosquito Aedes albopictus became of public health concern as it can replicate and transmit viral and filarial pathogens with a strong invasive success over the world. Various strategies have been proposed to reduce mosquito population's vectorial capacity. Among them, symbiotic control of mosquito borne disease offers promising perspectives. Such method is likely to be affected by the dynamics of mosquito-associated symbiotic communities, which might in turn be affected by host genotype and environment. Our previous study suggested a correlation between mosquitoes’ origin, genetic diversity and midgut bacterial diversity. To distinguish the impact of those factors, we have been studying the midgut bacterial microbiota of two Ae. albopictus populations from tropical (La Réunion) and temperate (Montpellier) origins under controlled laboratory conditions. the two populations experienced random mating or genetic bottleneck. Microbiota composition did not highlight any variation of the α and β-diversities in bacterial communities related to host’s populations. However, sizes of the mosquitoes were negatively correlated with the bacterial α-diversity of females. Variations in mosquito sex were associated with a shift in the composition of bacterial microbiota. The females’ mosquitoes also exhibited changes in the microbiota composition according to their size and after experiencing a reduction of their genetic diversity. These results provide a framework to investigate the impact of population dynamics on the symbiotic communities associated with the tiger mosquito.

Microbiota variations inCulex nigripalpusdisease vector mosquito of West Nile virus and Saint Louis Encephalitis from different geographic origins

“…Many factors including sex, age, size, geographic location, and other physiological status of mosquitoes have been shown to influence mosquito-associated microbiota (Osei-Poku et al, 2012; Zouache et al, 2010; Buck et al, 2016; Minard et al, 2018). Our study corroborated these previous findings and indicated that factors associated with different geographic locations might have impacted microbiota composition variations observed in adult female Cx.…”

Aedes spp. and Their Microbiota: A Review

“…The absence of a relation between population genetic origin and midgut microbiota was recently shown in two Ae. albopictus populations from tropical (La Réunion) and temperate (Montpellier, Continental France) regions reared under controlled laboratory conditions (Minard et al, 2018). These contrasting data highlight the need to continue investigating the impact of Aedes spp.…”

Evaluation of Two Different Solvents for Azolla pinnata Extracts on Chemical Compositions and Larvicidal Activity against Aedes albopictus (Diptera: Culicidae)

“…), hexadecanoic acid, and stigmast-4-en-3-one has the ability of antimicrobial properties when compared to major components of acetone solvent extracts. According to Minard et al [37], there was an interaction from all the midgut bacterial diversity for Ae. albopictus mosquitoes in their life cycle.…”