Shared idiotopes among monoclonal antibodies specific for A/PR/8/34 (H1N1) and X-31(H3N2) influenza viruses.

Moran, Thomas M.; Liu, Yung‐Nan C.; Schulman, Jerome L.; Bona, Constantin A.

doi:10.1073/pnas.81.6.1809

Cited by 22 publications

(17 citation statements)

References 24 publications

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“…After incubation and washing, the radioactivity was counted in a ß counter. As isotype controls, we used BALB/c monoclonal antibodies of various isotypes specific for hemagglutinin of PR8 influenza virus previously described (14).…”

Section: Methodsmentioning

confidence: 99%

Induction of Antihemagglutinin Antibodies by Polyclonal Antiidiotype Antibodies

Dincă

Neuwirth²,

Schulman³

et al. 1993

Viral Immunology

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Antiidiotypic antibodies can be envisioned as an alternative approach in the development of vaccines against influenza virus, which exhibits natural antigenic variations. In our work, we obtained two polyclonal cross-reactive anti-Id antibodies against PY102, VM113, and VM202 mAbs, which in turn are specific respectively for PR8 virus and laboratory-induced virus variants (PY102-V1 and VM113-V1). With these cross-reactive anti-Id antibodies, we were able to elicit anti-HA antibodies in mice. In comparing the anti-HA antibody response in animals injected with anti-Id antibodies to those immunized with PR8 influenza virus, we demonstrated that the HI titer was higher after virus immunization and that the PR8 virus boost was more efficient in this group. Our results showed that the polyclonal cross-reactive anti-Id antibodies were more efficient than the individual anti-Ids at eliciting responses. At the same time, we demonstrated that PR8-primed T cells, cultured with B cells from animals immunized with anti-Id antibodies, were able to produce anti-PR8 antibodies subsequent to stimulation with influenza virus.

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Section: Methodsmentioning

confidence: 99%

Induction of Antihemagglutinin Antibodies by Polyclonal Antiidiotype Antibodies

Dincă

Neuwirth²,

Schulman³

et al. 1993

Viral Immunology

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“…On the other hand, earlier observations of some of the coauthors demonstrated cross-reactive idiotopes on monoclonal antibodies to different epitopes on PR8 (H1) hemagglutinins and shared idiotypic determinants among antibodies to H1 and H3 hemagglutinins (12). These observations suggested the possibility that despite the diversity of clones responding to hemagglutinins, in some instances regulatory elements might operate to restrict the clonal population responding to particular antigenic determinants.…”

mentioning

confidence: 56%

“…As described in more detail previously, these antibodies have reciprocal binding properties and react with some viruses but not with variants selected in their presence, demonstrating that they possess paratopes specific for particular structures on an identical or a closely related topo-MATERIAL AND METHODS Viruses. Influenza A PR8 (A/PR/8/34, HiN1) virus and two sequential variants, PY102-Vl and VM113-V1, were grown in the allantoic cavity of 10-to 11-day-old embryonated chicken eggs and purified on 30-60%o sucrose gradients according to standard procedures (12).…”

mentioning

confidence: 99%

Nucleotide changes in sequential variants of influenza virus hemagglutinin genes and molecular structures of corresponding monoclonal antibodies specific for each variant.

Meek¹,

Johansson²,

Schulman³

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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We have generated four monoclonal antibodies specific for one or more members of a series of two sequentially derived PR8 influenza virus variants. Three of these antibodies share cross-reactive idiotypes. The amino acid sequences of these antibodies were determined, and it was found that two of these antibodies use genes from the VH7183 family, whereas the third uses a gene from the VHJ558 family.All four monoclonal antibodies derive from different families of genes encoding the variable region of the K chain. The RNA sequence of the parent PR8 virus as well as the RNA sequences of the sequential variants were also determined, and it was demonstrated that the variant hemagglutinin molecules differed from the parent molecule by only a single amino acid interchange. Despite these subtle differences in antigenic structures of hemagglutinin, and the cross-reactive idiotype of the antibodies, their primary structures were very different. These data reinforce the idea that a wide variety of antibody structures exist which are directed against subtly different structures in biologically important antigens. graphic region of hemagglutinin (11). They bear different idiotopes, clearly indicating they are produced by different clones (indeed the four antibodies were obtained from three separate fusions). However, three of the antibodies with distinguishable paratopes share a cross-reactive idiotype, again suggesting a potential connectivity among clones recognizing subtle differences in the hemagglutinin of related viruses.

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“…Spleen lymphocytes from immunized mice were fused with SP2/0 cells according to a previously described technique (3). Hybridomas secreting Abs which bound to Py203 and not to other monoclonal proteins of the same isotype were selected by a sensitive sandwich radioimmunoassay (RIA) according to a previously described technique (4). A hybrid was cloned under stringent limiting dilution conditions (0.5 cells/well) and a positive clone, RM1, was expanded.…”

Section: ) Preparation Of Synqeneic Monoclonal Anti-id Absmentioning

confidence: 99%

Effect of Syngeneic Anti-Idiotypic Antibody on Influenza Virus Neuraminidase Antibody Response

Mayer¹,

Ioannides²,

Moran³

et al. 1987

Viral Immunology

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Influenza viruses possess two major surface glycoproteins - hemagglutinin (HA) and neuraminidase (NA). Py203, a monoclonal antibody (Ab) specific for the neuraminidase of the PR8 (H1N1) influenza virus, was used to prepare syngeneic monoclonal anti-idiotypic (anti-Id) Abs. From a BALB/c mouse immunized with Py203 (anti-N1), we obtained RM1, a monoclonal anti-Id Ab. The Py203-Id was detected in a significant fraction of immunoglobulins (Igs) in the primary and secondary responses elicited by PR8 (H1N1) and X31 (H3N2) viruses. In animals injected with minute amounts of RM1 and subsequently boosted with an identical dose of RM1, no detectable anti-NA activity was noted, but a significant increase in Py203-Id-bearing Igs was observed. In the sera of animals injected with minute amounts of RM1 and subsequently boosted with PR8 (H1N1) or X31 (H3N2) viruses, an increase in anti-NA activity and in the level of Py203-Id was noted. Animals injected with large amounts of RM1 and boosted with PR8 and X31 showed a marked suppression of the Py203-Id but no alteration in the anti-NA response. The anti-Id recognizes an idiotope (the Py203 idiotope) shared by antibodies specific for the N1 and N2 neuraminidase variants.

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Shared idiotopes among monoclonal antibodies specific for A/PR/8/34 (H1N1) and X-31(H3N2) influenza viruses.

Cited by 22 publications

References 24 publications

Induction of Antihemagglutinin Antibodies by Polyclonal Antiidiotype Antibodies

Induction of Antihemagglutinin Antibodies by Polyclonal Antiidiotype Antibodies

Nucleotide changes in sequential variants of influenza virus hemagglutinin genes and molecular structures of corresponding monoclonal antibodies specific for each variant.

Effect of Syngeneic Anti-Idiotypic Antibody on Influenza Virus Neuraminidase Antibody Response

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