Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative Medicine

Hart, Melanie L.; Lauer, Jasmin C.; Selig, Mischa; Hanak, Martha; Walters, Brandan; Rolauffs, Bernd

doi:10.3390/jfmk3010002

Cited by 33 publications

(35 citation statements)

References 59 publications

(90 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Early response includes cell increased height followed, after 72 h, by a more flattened shape, while nuclei adopt a more circular form as their roundness increased. It is well accepted that shape parameters are instrumental in allowing specific cell functions and cell fate commitment towards differentiation [38][39][40].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts

Morabito

Guarnieri

Cucina

et al. 2020

IJMS

View full text Add to dashboard Cite

The effects induced by microgravity on human body functions have been widely described, in particular those on skeletal muscle and bone tissues. This study aims to implement information on the possible countermeasures necessary to neutralize the oxidative imbalance induced by microgravity on osteoblastic cells. Using the model of murine MC3T3-E1 osteoblast cells, cellular morphology, proliferation, and metabolism were investigated during exposure to simulated microgravity on a random positioning machine in the absence or presence of an antioxidant—the 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox). Our results confirm that simulated microgravity-induced morphological and metabolic alterations characterized by increased levels of reactive oxygen species and a slowdown of the proliferative rate. Interestingly, the use of Trolox inhibited the simulated microgravity-induced effects. Indeed, the antioxidant-neutralizing oxidants preserved cell cytoskeletal architecture and restored cell proliferation rate and metabolism. The use of appropriate antioxidant countermeasures could prevent the modifications and damage induced by microgravity on osteoblastic cells and consequently on bone homeostasis.

show abstract

Section: Discussionmentioning

confidence: 99%

Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts

Morabito

Guarnieri

Cucina

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Material stiffness controls many cell functions such as cell shape [ 42 ], adhesion [ 141 ], migration [ 137 ], differentiation [ 44 ], and proliferation [ 142 , 143 , 144 ]. Recently, cell morphology has received re-ignited attention, as measuring, predicting, and controlling cellular shape may aid in future regenerative medicine applications [ 145 ]. The morphology of MSCs is influenced by microenvironmental and biophysical stimuli [ 44 , 45 , 136 , 146 , 147 ] and is defined by how the cell balances external biomechanical forces with intracellular forces.…”

Section: Materials Stiffness-modulated Msc Shape and Lineage Determmentioning

confidence: 99%

Mechanotransduction and Stiffness-Sensing: Mechanisms and Opportunities to Control Multiple Molecular Aspects of Cell Phenotype as a Design Cornerstone of Cell-Instructive Biomaterials for Articular Cartilage Repair

Selig

Lauer

Hart

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Since material stiffness controls many cell functions, we reviewed the currently available knowledge on stiffness sensing and elucidated what is known in the context of clinical and experimental articular cartilage (AC) repair. Remarkably, no stiffness information on the various biomaterials for clinical AC repair was accessible. Using mRNA expression profiles and morphology as surrogate markers of stiffness-related effects, we deduced that the various clinically available biomaterials control chondrocyte (CH) phenotype well, but not to equal extents, and only in non-degenerative settings. Ample evidence demonstrates that multiple molecular aspects of CH and mesenchymal stromal cell (MSC) phenotype are susceptible to material stiffness, because proliferation, migration, lineage determination, shape, cytoskeletal properties, expression profiles, cell surface receptor composition, integrin subunit expression, and nuclear shape and composition of CHs and/or MSCs are stiffness-regulated. Moreover, material stiffness modulates MSC immuno-modulatory and angiogenic properties, transforming growth factor beta 1 (TGF-β1)-induced lineage determination, and CH re-differentiation/de-differentiation, collagen type II fragment production, and TGF-β1- and interleukin 1 beta (IL-1β)-induced changes in cell stiffness and traction force. We then integrated the available molecular signaling data into a stiffness-regulated CH phenotype model. Overall, we recommend using material stiffness for controlling cell phenotype, as this would be a promising design cornerstone for novel future-oriented, cell-instructive biomaterials for clinical high-quality AC repair tissue.

show abstract

“…In cell culture, myogenic stem cells assume spindle-shaped fibroblastic appearance, rapidly proliferate, and become confluent in less than one week [29]. Recently, cell shape has emerged as determinant, which controls cell proliferation, growth, physiology, and adaption for specific functions [30]. The cells also change their morphology in response to toxic stimuli.…”

Section: Resultsmentioning

confidence: 99%

Biocompatibility Evaluation and Enhancement of Elastomeric Coatings Made Using Table-Top Optical 3D Printer

et al. 2020

View full text Add to dashboard Cite

In this experimental report, the biocompatibility of elastomeric scaffold structures made via stereolithography employing table-top 3D printer Ember (Autodesk) and commercial resin FormLabs Flexible (FormLabs) was studied. The samples were manufactured using the standard printing and development protocol, which is known to inherit cytotoxicity due to remaining non-polymerized monomers, despite the polymerized material being fully biocompatible. Additional steps were taken to remedy this problem: the fabricated structures were soaked in isopropanol and methanol under different conditions (temperature and duration) to leach out the non-polymerized monomers. In addition, disc-shaped 3D-printed structures were UV exposed to assure maximum polymerization degree of the material. Post-processed structures were seeded with myogenic stem cells and the number of live cells was evaluated as an indicator for the material biocompatibility. The straightforward post-processing protocol enhanced the biocompatibility of the surfaces by seven times after seven days soaking in isopropanol and methanol and was comparable to control (glass and polystyrene) samples. This proposes the approach as a novel and simple method to be widely applicable for dramatic cytotoxicity reduction of optically 3D printed micro/nano-scaffolds for a wide range of biomedical studies and applications.

show abstract

Shaping the Cell and the Future: Recent Advancements in Biophysical Aspects Relevant to Regenerative Medicine

Cited by 33 publications

References 59 publications

Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts

Antioxidant Strategy to Prevent Simulated Microgravity-Induced Effects on Bone Osteoblasts

Mechanotransduction and Stiffness-Sensing: Mechanisms and Opportunities to Control Multiple Molecular Aspects of Cell Phenotype as a Design Cornerstone of Cell-Instructive Biomaterials for Articular Cartilage Repair

Biocompatibility Evaluation and Enhancement of Elastomeric Coatings Made Using Table-Top Optical 3D Printer

Contact Info

Product

Resources

About