Shaping the actin cytoskeleton using microtubule tips

Basu, Roshni; Chang, Fred

doi:10.1016/j.ceb.2006.12.012

Cited by 69 publications

(70 citation statements)

References 63 publications

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“…These findings are consistent with an earlier report that microtubules were required for efficient DRP1C and CLC dynamics (Konopka et al, 2008). Given the connection between formin and microtubule plus-ends and the link between formin and microfilaments (Cheung and Wu, 2004;Basu and Chang, 2007), we suggest that alterations in microtubule dynamics led directly to a redistribution of Flot1, which in turn would regulate the velocity and positioning of Flot1-positive puncta.…”

Section: Membrane Microdomains and The Cytoskeleton Are Required For supporting

confidence: 81%

A Membrane Microdomain-Associated Protein, Arabidopsis Flot1, Is Involved in a Clathrin-Independent Endocytic Pathway and Is Required for Seedling Development

et al. 2012

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Endocytosis is essential for the maintenance of protein and lipid compositions in the plasma membrane and for the acquisition of materials from the extracellular space. Clathrin-dependent and -independent endocytic processes are well established in yeast and animals; however, endocytic pathways involved in cargo internalization and intracellular trafficking remain to be fully elucidated for plants. Here, we used transgenic green fluorescent protein-flotillin1 (GFP-Flot1) Arabidopsis thaliana plants in combination with confocal microscopy analysis and transmission electron microscopy immunogold labeling to study the spatial and dynamic aspects of GFP-Flot1-positive vesicle formation. Vesicle size, as outlined by the gold particles, was ;100 nm, which is larger than the 30-nm size of clathrin-coated vesicles. GFP-Flot1 also did not colocalize with clathrin light chainmOrange. Variable-angle total internal reflection fluorescence microscopy also revealed that the dynamic behavior of GFPFlot1-positive puncta was different from that of clathrin light chain-mOrange puncta. Furthermore, disruption of membrane microdomains caused a significant alteration in the dynamics of Flot1-positive puncta. Analysis of artificial microRNA Flot1 transgenic Arabidopsis lines established that a reduction in Flot1 transcript levels gave rise to a reduction in shoot and root meristem size plus retardation in seedling growth. Taken together, these findings support the hypothesis that, in plant cells, Flot1 is involved in a clathrin-independent endocytic pathway and functions in seedling development.

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Section: Membrane Microdomains and The Cytoskeleton Are Required For supporting

confidence: 81%

A Membrane Microdomain-Associated Protein, Arabidopsis Flot1, Is Involved in a Clathrin-Independent Endocytic Pathway and Is Required for Seedling Development

et al. 2012

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“…Moreover, several observations suggest that altering the microtubule network results in altered RhoA activity and/ or localization, which in turn could contribute to altered cell motility (reviewed in ref. 49). Thus, it is conceivable that p27 could indirectly contribute to regulating RhoA activity through its interaction with stathmin and the consequent modulation of microtubule dynamics.…”

Section: Discussionmentioning

confidence: 99%

p27Kip1 expression inhibits glioblastoma growth, invasion, and tumor-induced neoangiogenesis

Schiappacassi¹,

Lovat²,

Canzonieri

et al. 2008

Molecular Cancer Therapeutics

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“…MT act to induce cell polarity formation by targeting and/or locally activating signaling molecules at the polar site in yeast and animal cells (Basu & Chang 2007, Siegrist & Doe 2007. A common signaling molecule regulated by MT in these systems is a Rho family GTPase, which controls actin-based polarity formation (Basu & Chang 2007, Siegrist & Doe 2007.…”

Section: Microtubulesmentioning

confidence: 99%

Cell Polarity Signaling in Arabidopsis

Yang

2008

Annu. Rev. Cell Dev. Biol.

253

237

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Cell polarization is intimately linked to plant development, growth, and responses to the environment. Major advances have been made in our understanding of the signaling pathways and networks that regulate cell polarity in plants owing to recent studies on several model systems, e.g., tip growth in pollen tubes, cell morphogenesis in the leaf epidermis, and polar localization of PINs. From these studies we have learned that plant cells use conserved mechanisms such as Rho family GTPases to integrate both plant-specific and conserved polarity cues and to coordinate the cytoskeketon dynamics/reorganization and vesicular trafficking required for polarity establishment and maintenance. This review focuses upon signaling mechanisms for cell polarity formation in Arabidopsis, with an emphasis on Rho GTPase signaling in polarized cell growth and how these mechanisms compare with those for cell polarity signaling in yeast and animal systems.

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Shaping the actin cytoskeleton using microtubule tips

Cited by 69 publications

References 63 publications

A Membrane Microdomain-Associated Protein, Arabidopsis Flot1, Is Involved in a Clathrin-Independent Endocytic Pathway and Is Required for Seedling Development

A Membrane Microdomain-Associated Protein, Arabidopsis Flot1, Is Involved in a Clathrin-Independent Endocytic Pathway and Is Required for Seedling Development

p27Kip1 expression inhibits glioblastoma growth, invasion, and tumor-induced neoangiogenesis

Cell Polarity Signaling in Arabidopsis

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