Shaping microbial consortia in coupling glycerol fermentation and carboxylate chain elongation for Co-production of 1,3-propanediol and caproate: Pathways and mechanisms

Leng, Ling; Nobu, Masaru K.; Narihiro, Takashi; Yang, Peixian; Tan, Giin-Yu Amy; Lee, Po‐Heng

doi:10.1016/j.watres.2018.10.063

Cited by 35 publications

(16 citation statements)

References 41 publications

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“…This absence of propionate may be related to the initial presence of acetate at concentrations sufficient to form even-chain elongation products such as caproate. Leng et al (2019) also confirmed that the production of even-chain compounds from glycerol can occur together with the production of 1,3-PDO. This occurs due to the presence of ethanol in the medium, whether from the conversion of crude glycerol or when externally added, as in the present investigation.…”

Section: Effect Of Caprogenic Sludge Addition On Ethanol Consumption and Bioproduct Productionsupporting

confidence: 60%

“…Anaerobic processes generally have lower operating costs compared to those of other bioprocesses, and they offer a wide range of value-added products. There are several interesting industrial products that can be obtained via anaerobic digestion of crude glycerol, such as hydrogen and methane and alcohols such as ethanol and butanol, 1,3-propanediol (1,3-PDO), polyhydroxyalkanoates, and polyols that are commonly applied in chemical, cosmetics, food, and pharmaceutical industries (Burniol-Figols et al 2018;Monteiro et al 2018); and carboxylic acids as succinate, acetate, propionate, butyrate, and caproate (Silva et al 2009;Leng et al 2017Leng et al , 2019Monteiro et al 2018). These products can be obtained with either continuous or discontinuous systems, and with pure or open cultures.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to C. kluyveri, other microorganisms have been mentioned in studies involving chain elongation reactions of even and odd chains, such as those belonging to the genera Clostridium, Ruminococcus, Megasphaera, and Eubacterium (Weimer et al 2015;Angenent et al 2016;Kucek et al 2016a;Cavalcante et al 2017;Wu et al 2019). In the case of MCCA formation from crude glycerol, open cultures with dominance of Clostridium groups can be involved in the chain elongation, as well as in 1,3-PDO production (Leng et al 2017(Leng et al , 2019. Consequently, the 1,3-PDO formation may interfere in the oxidative pathway resulting in lower yields of products from chain elongation processes.…”

Section: Introductionmentioning

confidence: 99%

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Anaerobic production of valeric acid from crude glycerol via chain elongation

Veras

Cavalcante

Gehring

et al. 2019

Int. J. Environ. Sci. Technol.

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Glycerol from biodiesel production was used as a substrate for valerate production in a 13.1-L anaerobic filter with an open microbiome. Ethanol at 15% of the influent chemical oxygen demand was supplemented as an additional electron donor source. The 114-day experimental period was divided in three phases which included initial adaptation phase, external sludge addition from a caproic acid producing reactor and valerate extraction using a pertraction system. Propionate (0.63-1.75 g COD L −1 day −1 ) and valerate (0.86-1.81 g COD L −1 day −1 ) were the main carboxylates formed throughout all operational phases. An increase in production rates of butyrate (0.26-0.31 g COD L −1 day −1 ), caproate (0.01-0.08 g COD L −1 day −1 ), and 1,3-propanediol (0.43-0.52 g COD L −1 day −1 ) was observed with addition of external caproic acid producing sludge rich in Clostridium members. In the operational phase with pertraction, a sudden decrease in 1,3-propanediol and concomitant increase in acid production were verified. Propionate and valerate reached higher production rates compared with the other two phases, suggesting that the pertraction system favored the oxidative pathway of glycerol fermentation. Valerate extraction reached a maximum of 30 g COD m −2 day −1 . The filter microbiome was highly diverse with Simpson index values close to 0.1 for the four sludge samples collected, and a concomitant increase in Megasphaera elsdenii and an increase in valerate production rates were observed. Hence, high valerate production and extraction rates through the carboxylate platform are a feasible alternative for crude glycerol valorization with a great potential for improvement in future research.

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Section: Effect Of Caprogenic Sludge Addition On Ethanol Consumption and Bioproduct Productionsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anaerobic production of valeric acid from crude glycerol via chain elongation

Veras

Cavalcante

Gehring

et al. 2019

Int. J. Environ. Sci. Technol.

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“…MCCs in general can be used in the production of e.g., solvents, feed additives (Liu et al, 2018), lubricants, surfactants, emulsifiers, pharmaceuticals (Angenent et al, 2016) and as precursors for plastics and fuels (Levy et al, 1984;Zhang et al, 2004). The amount of research on the formation of straight MCCs such as n-caproate (n-C 6 ) and n-caprylate (n-C 8 ) via microbial chain elongation is quickly expanding (Steinbusch et al, 2011;Zhu et al, 2015;Angenent et al, 2016;Zhu et al, 2017;Chwialkowska et al, 2019;Han et al, 2019;Leng et al, 2019). Chain elongation fermentation technology could become an impactful recycling method that can aid in the development of a circular economy (De Groof et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Open Culture Ethanol-Based Chain Elongation to Form Medium Chain Branched Carboxylates and Alcohols

Leeuw

Ahrens

Buisman

et al. 2021

Front. Bioeng. Biotechnol.

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Chain elongation fermentation allows for the synthesis of biobased chemicals from complex organic residue streams. To expand the product spectrum of chain elongation technology and its application range we investigated 1) how to increase selectivity towards branched chain elongation and 2) whether alternative branched carboxylates such as branched valerates can be used as electron acceptors. Elongation of isobutyrate elongation towards 4-methyl-pentanoate was achieved with a selectivity of 27% (of total products, based on carbon atoms) in a continuous system that operated under CO2 and acetate limited conditions. Increasing the CO2 load led to more in situ acetate formation that increased overall chain elongation rate but decreased the selectivity of branched chain elongation. A part of this acetate formation was related to direct ethanol oxidation that seemed to be thermodynamically coupled to hydrogenotrophic carboxylate reduction to corresponding alcohols. Several alcohols including isobutanol and n-hexanol were formed. The microbiome from the continuous reactor was also able to form small amounts of 5-methyl-hexanoate likely from 3-methyl-butanoate and ethanol as substrate in batch experiments. The highest achieved concentration of isoheptanoate was 6.4 ± 0.9 mM Carbon, or 118 ± 17 mg/L, which contributed for 7% to the total amount of products (based on carbon atoms). The formation of isoheptanoate was dependent on the isoform of branched valerate. With 3-methyl-butanoate as substrate 5-methylhexanoate was formed, whereas a racemic mixture of L/D 2-methyl-butanoate did not lead to an elongated product. When isobutyrate and isovalerate were added simultaneously as substrates there was a large preference for elongation of isobutyrate over isovalerate. Overall, this work showed that chain elongation microbiomes can be further adapted with supplement of branched-electron acceptors towards the formation of iso-caproate and iso-heptanoate as well as that longer chain alcohol formation can be stimulated.

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“…The amount of research groups that study the formation of straight MCCs such as ncaproate (n-C6) and n-caprylate (n-C8) via microbial chain elongation is quickly expanding 22,185,[233][234][235][236][237][238] . Commercial application by ChainCraft B.V. in Amsterdam will demonstrate large scale production of n-C6 from organic residues 239 .…”

Section: Introductionmentioning

confidence: 99%

Open culture chain elongation for branched carboxylate formation

Leeuw¹,

Daniël²

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How to create an environment that selects for decision making to favor the development of a circular economy, however, is a very complex endeavor and fortunately does not fall any further into the scope of this thesis. Inside the scope of this thesis is a much simpler research on how to use mixed culture biotechnology principles to enrich microbiomes that can be used to recycle organic residue streams via production of useful biochemicals. elongated with an acetyl-CoA unit in a Claisen condensation reaction that is catalyzed by a thiolase 34 . The acetyl-CoA elongation unit is usually derived from oxidation of the electron donor, whereas electrons from this oxidation are used to reduce the elongation product after condensation. It results in an elongated carboxylate as metabolic end-product that has been extended by two hydrated carbon atoms. However, limiting the bicarbonate availability too much will also affect the growth of ethanol based chain elongator: Clostridium kluyveri, because it requires bicarbonate for biomass formation 53 . For methanol based chain elongation bicarbonate can be a direct substrate for the formation of acetate and butyrate 43 . Though, recent research has also shown that methanol based chain elongation of acetate, without the utilization of bicarbonate, is possible 45 . Bicarbonate dosing in this case should be dependent on the desired methanol based chain elongation conversions. Another important selection pressure tool is the possibility to enforce a certain minimum growth rate by setting the hydraulic retention time of a reactor during continuous operation 15 Acetoclastic methanogenesis 69 CH3COO -+ H2O  CH4 + HCO3 --31.05 -31.05 -31.05 ΔG 0' biological standard conditions (pH 7) ΔG 1 hydrogen partial pressure = 1 kPa ΔG 2 hydrogen partial pressure = 1 Pa *

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Shaping microbial consortia in coupling glycerol fermentation and carboxylate chain elongation for Co-production of 1,3-propanediol and caproate: Pathways and mechanisms

Cited by 35 publications

References 41 publications

Anaerobic production of valeric acid from crude glycerol via chain elongation

Anaerobic production of valeric acid from crude glycerol via chain elongation

Open Culture Ethanol-Based Chain Elongation to Form Medium Chain Branched Carboxylates and Alcohols

Open culture chain elongation for branched carboxylate formation

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