Shaping membrane interfaces in lipid vesicles mimicking the cytoplasmic leaflet of myelin through variation of cholesterol and myelin basic protein contents

Träger, Jennica; Meister, Annette; Hause, Gerd; Harauz, George; Hinderberger, Dariush

doi:10.1016/j.bbamem.2023.184179

Cited by 3 publications

(4 citation statements)

References 60 publications

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“…Hence, we added MBP, which has been amply characterized in-detail by our group in recent years, both in aqueous solution as well as in phospholipid-based structures like large unilamellar vesicles (LUVs), lipid nanodiscs, and phospholipid monolayers. 45,46 MBP and Aqueous IL Nanostructures. After identifying water/IL mixing ratios promising to harbor well-defined nanostructures, further experiments were performed to test the behavior of MBP in these mixtures.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…As we aimed at exploring new applications for aqueous/IL mixtures based on their intrinsic nanostructure, we decided to use the aqueous 45 and 50 vol % BMImBF 4 mixing ratios for tests as potential model-membrane systems. Hence, we added MBP, which has been amply characterized in-detail by our group in recent years, both in aqueous solution as well as in phospholipid-based structures like large unilamellar vesicles (LUVs), lipid nanodiscs, and phospholipid monolayers. , …”

Section: Results and Discussionmentioning

confidence: 99%

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Aqueous Ionic Liquid Mixtures as Minimal Models of Lipid Bilayer Membranes

Volmer,

Cerajewski,

Alfes

et al. 2024

ACS Biomater. Sci. Eng.

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We introduce aqueous ionic liquid (IL) mixtures, specifically mixtures of 1-butyl-3-imidazoliumtetrafluoroborate (BMImBF4), with water as a minimal model of lipid bilayer membranes. Imidazolium-based ILs are known to form clustered nanoscale structures in which local inhomogeneities, micellar or lamellar structures, are formed to shield hydrophobic parts of the cation from the polar cosolvent (water). To investigate these nanostructures, dynamic light scattering (DLS) on samples with different mixing ratios of water and BMImBF4 was performed. At mixing ratios of 50% and 45% (v/v), small and homogeneous nanostructures can indeed be detected. To test whether, in particular, these stable nanostructures in aqueous mixtures may mimic the effects of phospholipid bilayer membranes, we further investigated their interaction with myelin basic protein (MBP), a peripheral, intrinsically disordered membrane protein of the myelin sheath. Using dynamic light scattering (DLS), continuous wave (CW) and pulse electron paramagnetic resonance (EPR), and small-angle X-ray scattering (SAXS) on recombinantly produced, “healthy” charge variants rmC1WT and double cysteine variant C1S17CH85C, we find that the size and the shape of the determined nanostructures in an optimum mixture offer model membranes in which the protein exhibits native behavior. SAXS measurements illuminate the size and shape of the nanostructures and indicate IL-rich “beads” clipped together by functional MBP, one of the in vivo roles of the protein in the myelin sheath. All the gathered data combined indicate that the 50% and 45% aqueous IL mixtures can be described as offering minimal models of a lipid mono- or bilayer that allow native processing and potential study of at least peripheral membrane proteins like MBP.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

Aqueous Ionic Liquid Mixtures as Minimal Models of Lipid Bilayer Membranes

Volmer,

Cerajewski,

Alfes

et al. 2024

ACS Biomater. Sci. Eng.

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“…Lipid vesicles exhibit considerable diversity in their shape, surface electrical charge and lipid composition, factors that influence variances in permeability across biological membranes [ 62 , 63 , 64 ]. The configuration of the lipid bilayer has a pivotal role in regulating the fluidity of the vesicle membrane.…”

Section: Methodsmentioning

confidence: 99%

Chitosan Soft Matter Vesicles Loaded with Acetaminophen as Promising Systems for Modified Drug Release

Hilițanu,

Mititelu-Tarțău,

Popa

et al. 2023

Molecules

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Our study was designed to acquire, characterize and evaluate the biocompatibility of novel lipid vesicles loaded with acetaminophen (APAP) and coated with chitosan (CS). We investigated the in vitro and in vivo drug release kinetics from these systems, and we conducted assessments for both in vitro hemocompatibility and in vivo biocompatibility. For the in vivo biocompatibility evaluation, the mice were randomly divided into four groups of six animals and were treated orally as follows: control group: 0.1 mL/10 g body weight of double-distilled water; CS group: 0.1 mL/10 g body weight 1% CS solution; APAP group: 150 mg/kg body weight APAP; APAP-v group: 150 mg/kg body weight APAP-loaded lipid vesicles. The impact of APAP-v on various hematological, biochemical, and immune parameters in mice were assessed, and the harvested tissues were subjected to histopathological examination. The innovative formulations effectively encapsulating APAP within soft vesicles exhibited reasonable stability in solution and prolonged drug release in both in vitro and in vivo studies. The in vitro hemolysis test involving APAP-loaded vesicles revealed no signs of damage to red blood cells. The mice treated with APAP-v showed neither significant variances in hematological, biochemical, and immune parameters, nor structural changes in the examined organ samples, compared to the control group. APAP-v administration led to prolonged drug release. We can conclude that the APAP-v are innovative carrier systems for modifying drug release, making them promising candidates for biomedical applications.

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“…The vesicle bilayer is a simplified model used to study the cell membrane system in vitro ( Träger et al, 2023 ), and the curvature and size of the bilayer are close to the real cell membrane. However, the composition of the bilayer is difficult to control precisely, and information about the thermodynamic characteristics of the membrane system cannot be obtained.…”

Section: Introductionmentioning

confidence: 99%

Effect of high sodium ion level on the interaction of AmB with a cholesterol-rich phospholipid monolayer

Wang,

Qiang,

et al. 2024

Front. Mol. Biosci.

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Invasive fungal infections are a primary reason for high mortality in immunocompromised people, especially in critically ill patients, such as intensive care unit (ICU) patients, advanced cancer patients, or severe burn patients. Hypernatremia also can increase mortality in severely ill patients. Amphotericin B (AmB) is the gold standard for treating infections, but in severely ill patients, AmB can cause hematotoxicity when administered intravenously due to its interaction with cholesterol on red blood cell membranes. This results in limited doses of AmB and affects the treatment of infections. The proportion of cholesterol molecules in membrane lipids in red blood cells is as high as 50 mol%, and the sodium ions can influence the interaction between AmB and lipids on the membrane. Therefore, in the complex clinical situation of a severely ill patient with a fungal infection and hypernatremia, the interaction between amphotericin B and the red blood cell membranes is worth studying in depth. In this work, the interaction between AmB and the dipalmitoyl phosphatidylcholine (DPPC)/cholesterol mixed monolayer in the presence of high sodium ion levels was studied when the proportion of cholesterol was 50%. The results show that the effect of AmB on reducing the monolayer’s area at a high level of sodium ions is slightly stronger at 30 mN/m. The effect of AmB on reducing the elastic modulus of the DPPC/Chol monolayer is significantly weakened by a high sodium ion level, compared with the level of sodium ions at normal physiological concentration. The higher the sodium ion concentration, the weaker the intermolecular force of the DPPC/Chol/AmB mixed monolayers. The scanning electron microscope (SEM) and atomic force microscopy (AFM) observations suggest that at a high sodium ion level, the presence of AmB significantly reduces the surface roughness of the DPPC/Chol monolayer. AmB may bind to cholesterol molecules, and it isolates cholesterol from the monolayer, resulting in a reduced height of the cholesterol-rich monolayer and an increasingly dispersed monolayer region. The results are beneficial to understanding the mechanism of impact of a high sodium ion level on the relationship between AmB and red blood cell membranes rich in cholesterol and are valuable for understanding the hemolytic toxicity of AmB to red blood cells at a high sodium ion level.

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Shaping membrane interfaces in lipid vesicles mimicking the cytoplasmic leaflet of myelin through variation of cholesterol and myelin basic protein contents

Cited by 3 publications

References 60 publications

Aqueous Ionic Liquid Mixtures as Minimal Models of Lipid Bilayer Membranes

Aqueous Ionic Liquid Mixtures as Minimal Models of Lipid Bilayer Membranes

Chitosan Soft Matter Vesicles Loaded with Acetaminophen as Promising Systems for Modified Drug Release

Effect of high sodium ion level on the interaction of AmB with a cholesterol-rich phospholipid monolayer

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