Shaping colour changes in a biofilm-forming cyanobacterium by modifying the culture conditions

Prieto, B.; Vázquez-Nion, Daniel; Silva, B.; Sanmartín, Patricia

doi:10.1016/j.algal.2018.05.010

Cited by 7 publications

(3 citation statements)

References 26 publications

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“…In the case of P(3HB)-coated concrete, biofilm formation was visibly evident across all treatments, showcasing a more diverse microbial composition and higher abundance compared to both control concretes, as indicated by the distinct colour and bacterial counts (Figs 5 and 6 ). The colour intensity of the biofilm can serve as a basic yet effective quantitative method for assessing its development, as it is influenced by the presence of pigments like chlorophyll a, carotenoids, and phycobiliproteins [ 73 , 74 ]. While exact bacterial counts in a laboratory setting is impossible, the retrieval of particle-associated bacteria, with a cultivability as high as 25% of the population, can be achieved using the well-established Zobell Marine agar [ 75 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of poly(3-hydroxybutyrate) [P(3HB)] coating on the bacterial communities of artificial structures

Chai,

Syauqi,

Sudesh

et al. 2024

PLoS ONE

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The expanding urbanization of coastal areas has led to increased ocean sprawl, which has had both physical and chemical adverse effects on marine and coastal ecosystems. To maintain the health and functionality of these ecosystems, it is imperative to develop effective solutions. One such solution involves the use of biodegradable polymers as bioactive coatings to enhance the bioreceptivity of marine and coastal infrastructures. Our study aimed to explore two main objectives: (1) investigate PHA-degrading bacteria on polymer-coated surfaces and in surrounding seawater, and (2) comparing biofilm colonization between surfaces with and without the polymer coating. We applied poly(3-hydroxybutyrate) [P(3HB)) coatings on concrete surfaces at concentrations of 1% and 6% w/v, with varying numbers of coating cycles (1, 3, and 6). Our findings revealed that the addition of P(3HB) indeed promoted accelerated biofilm growth on the coated surfaces, resulting in an occupied area approximately 50% to 100% larger than that observed in the negative control. This indicates a remarkable enhancement, with the biofilm expanding at a rate roughly 1.5 to 2 times faster than the untreated surfaces. We observed noteworthy distinctions in biofilm growth patterns based on varying concentration and number of coating cycles. Interestingly, treatments with low concentration and high coating cycles exhibited comparable biofilm enhancements to those with high concentrations and low coating cycles. Further investigation into the bacterial communities responsible for the degradation of P(3HB) coatings identified mostly common and widespread strains but found no relation between the concentration and coating cycles. Nevertheless, this microbial degradation process was found to be highly efficient, manifesting noticeable effects within a single month. While these initial findings are promising, it’s essential to conduct tests under natural conditions to validate the applicability of this approach. Nonetheless, our study represents a novel and bio-based ecological engineering strategy for enhancing the bioreceptivity of marine and coastal structures.

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Section: Discussionmentioning

confidence: 99%

Effects of poly(3-hydroxybutyrate) [P(3HB)] coating on the bacterial communities of artificial structures

Chai,

Syauqi,

Sudesh

et al. 2024

PLoS ONE

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“…However, it was Prieto et al [210] who, for the first time, proved the correlation between modifications in the number of organisms and changes in the parameters defining color. Indeed, most works [199,211,212,213,214] performed color measurements using the CIELAB color system [215], which represents each color by means of three scalar parameters: L*, lightness or luminosity of color; a*, associated with changes in redness-greenness; and b*, associated with changes in yellowness-blueness. Each color can also be represented by three angular parameters: L*, lightness or luminosity of color, defined in both scalar and angular color sets; C* ab , chroma or saturation, related to the intensity of color; and h ab , hue angle or tone of color, which refers to the dominant wavelength [214].…”

Section: Quantitative Analysis Of Biofilm On Anti-biofilm Polymerimentioning

confidence: 99%

Testing Anti-Biofilm Polymeric Surfaces: Where to Start?

Cattò

Cappitelli

2019

IJMS

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Present day awareness of biofilm colonization on polymeric surfaces has prompted the scientific community to develop an ever-increasing number of new materials with anti-biofilm features. However, compared to the large amount of work put into discovering potent biofilm inhibitors, only a small number of papers deal with their validation, a critical step in the translation of research into practical applications. This is due to the lack of standardized testing methods and/or of well-controlled in vivo studies that show biofilm prevention on polymeric surfaces; furthermore, there has been little correlation with the reduced incidence of material deterioration. Here an overview of the most common methods for studying biofilms and for testing the anti-biofilm properties of new surfaces is provided.

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“…On the other hand, many commercially produced coatings are tested exclusively according to the manufacturer's internal guidelines, and the existing norms currently serve only as recommendations, with producers not obliged to test for biological corrosion [11]. Although many methods for the determination of photoautotrophic growth on inorganic materials and cultural objects have already been evaluated [8][9][10][22][23][24], these have not yet been widely incorporated for testing manufactured plaster-based coatings. Additionally, some techniques are inadequate for testing a large number of highly differentiated sets of samples, or require costly, highly specialized equipment.…”

Section: Introductionmentioning

confidence: 99%

Development of a Method for Assessing the Resistance of Building Coatings to Phoatoautotrophic Biofouling

Komar,

Szulc,

Kata

et al. 2023

Applied Sciences

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The aim of this study was to develop a method for assessing the growth of photoautotrophs on plaster coatings, which will be used to reliably assess the resistance of these materials to photoautotrophic growth in the simulation of long-term exposure. In the course of the study, mineral and silicone plaster substrates were inoculated with a mixture of Stichococcus bacillaris, Nostoc commune, Pseudochlorella signiensis, and Coenochloris signiensis, and incubated for 28 days in model conditions. At 14 and 28 days after inoculation, the degree of photoautotrophic growth was determined using hemocytometer cell counting, a HY-LiTE 2 ATP measuring system, chlorophyll a concentration quantification, CIE L*a*b spectrophotometric color change evaluation, and visual assessment. The acquired results allowed us to select visual assessment and spectrophotometric color change evaluation as quick-to-perform and reliable techniques for further laboratory studies. The impact of minor changes introduced in the inoculation and incubation procedures on the rate of biofilm formation and severity of microbial fouling was studied. Differences in inoculation and incubation procedures strongly affected the results of the performed tests. Both methods have shown high potential and should be further expanded upon in environmental studies.

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Shaping colour changes in a biofilm-forming cyanobacterium by modifying the culture conditions

Cited by 7 publications

References 26 publications

Effects of poly(3-hydroxybutyrate) [P(3HB)] coating on the bacterial communities of artificial structures

Effects of poly(3-hydroxybutyrate) [P(3HB)] coating on the bacterial communities of artificial structures

Testing Anti-Biofilm Polymeric Surfaces: Where to Start?

Development of a Method for Assessing the Resistance of Building Coatings to Phoatoautotrophic Biofouling

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