Shape remodeling and blebbing of active cytoskeletal vesicles

Loiseau, Etienne; Schneider, Jochen; Keber, Felix C.; Pelzl, Carina; Massiera, Gladys; Salbreux, Guillaume; Bausch, Andreas R.

doi:10.1126/sciadv.1500465

Cited by 88 publications

(103 citation statements)

References 41 publications

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“…Here we elucidate the role of the presence of a cytoskeletal cortex on the adhesion process. We show that a strong coupling of the actin network to the membrane causes dampening of the membrane fluctuations and an increase in membrane tension (15,16). When a tensed cytoskeletal vesicle binds specifically to a glass surface using biotin streptavidin as ligand-receptor pair the membrane tension increases beyond the lysis tension.…”

Section: Introductionmentioning

confidence: 92%

“…Vesicles were produced using the cDICE method (16,18). Briefly, this method consists of a cylindrical rotating chamber successively filled with a glucose solution to collect the vesicles, a lipid-in-oil solution to saturate the oil/water (O/W) interfaces, and decane as the continuous phase in which droplets were produced.…”

Section: Vesicle Productionmentioning

confidence: 99%

“…Briefly, this method consists of a cylindrical rotating chamber successively filled with a glucose solution to collect the vesicles, a lipid-in-oil solution to saturate the oil/water (O/W) interfaces, and decane as the continuous phase in which droplets were produced. The protocol to disperse the lipids in the oil solution has been published elsewhere (16). The solution containing the cytoskeletal elements was injected from a glass capillary tube by inserting the capillary's tip in the decane.…”

Section: Vesicle Productionmentioning

confidence: 99%

“…1(a)). Protein encapsulation occurs during vesicle preparation using the cDICE method adapted for this system (16). By adding myosin motors to the network, we add contractility and hence activity.…”

Section: Cytoskeletal Vesiclesmentioning

confidence: 99%

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Adhesion of active cytoskeletal vesicles

Maan¹,

Loiseau²,

Bausch³

2018

Preprint

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Regulation of adhesion is a ubiquitous feature of living cells, observed during processes such as motility, antigen recognition or rigidity sensing. At the molecular scale, a myriad of mechanisms are necessary to recruit and activate the essential proteins, while at the cellular scale efficient regulation of adhesion relies on the cell's ability to adapt its global shape. To understand the role of shape remodeling during adhesion, we use a synthetic biology approach to design a minimal model, starting with a limited number of building blocks. We assemble cytoskeletal vesicles whose size, reduced volume, and cytoskeleton contractility can be independently tuned. We are able to show that these cytoskeletal vesicles can sustain strong adhesion to solid substrates only if molecular motors are able to actively remodel the actin cortex. When the cytoskeletal vesicles are deformed under hypertonic osmotic pressure, they develop a crumpled geometry with huge deformations. In the presence of molecular motors, these deformations are dynamic in nature and can compensate for an absence of excess membrane area needed for adhesion to take place. When the cytoskeletal deformations are able to compensate for lack of excess membrane area, the cytoskeletal vesicles are able to attach to the rigid glass surfaces even under strong adhesive forces. The balance of deformability and adhesion strength is identified to be key to enable cytoskeletal vesicles to adhere to solid substrates.

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Section: Introductionmentioning

confidence: 92%

Section: Vesicle Productionmentioning

confidence: 99%

Section: Vesicle Productionmentioning

confidence: 99%

Section: Cytoskeletal Vesiclesmentioning

confidence: 99%

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Adhesion of active cytoskeletal vesicles

Maan¹,

Loiseau²,

Bausch³

2018

Preprint

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“…However, by this technique, the incorporation of cholesterol into vesicle membranes can hardly be obtained. Recently, Bausch and his colleagues applied the cDICE technique to fabricate a series of vesicles . By this modified emulsion transfer technique, they have developed a cytoskeletal vesicle.…”

Section: Microfluidics For the Fabrication Of Vesiclesmentioning

confidence: 99%

Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

Xie

Xiong

et al. 2019

Small

116

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Fabrication of artificial biomimetic materials has attracted abundant attention. As one of the subcategories of biomimetic materials, artificial cells are highly significant for multiple disciplines and their synthesis has been intensively pursued. In order to manufacture robust “alive” artificial cells with high throughput, easy operation, and precise control, flexible microfluidic techniques are widely utilized. Herein, recent advances in microfluidic‐based methods for the synthesis of droplets, vesicles, and artificial cells are summarized. First, the advances of droplet fabrication and manipulation on the T‐junction, flow‐focusing, and coflowing microfluidic devices are discussed. Then, the formation of unicompartmental and multicompartmental vesicles based on microfluidics are summarized. Furthermore, the engineering of droplet‐based and vesicle‐based artificial cells by microfluidics is also reviewed. Moreover, the artificial cells applied for imitating cell behavior and acting as bioreactors for synthetic biology are highlighted. Finally, the current challenges and future trends in microfluidic‐based artificial cells are discussed. This review should be helpful for researchers in the fields of microfluidics, biomaterial fabrication, and synthetic biology.

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Learning from Embryo Development to Engineer Self‐organizing Materials

Senoussi¹,

Vyborna²,

Berthoumieux³

et al. 2021

Out‐of‐Equilibrium (Supra)molecular Systems and Materials

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Shape remodeling and blebbing of active cytoskeletal vesicles

Abstract: Cell-sized biomimetic active cytoskeletal vesicles undergo blebbing and shape remodeling due to myosin contractile activity.

Cited by 88 publications

References 41 publications

Adhesion of active cytoskeletal vesicles

Adhesion of active cytoskeletal vesicles

Microfluidics for Biosynthesizing: from Droplets and Vesicles to Artificial Cells

Learning from Embryo Development to Engineer Self‐organizing Materials

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