Shape‐Dependent Optoelectronic Cell Lysis

The lysis of cells in order to extract the nucleic acids or proteins inside it is a crucial unit operation in biomolecular analysis. This paper presents a critical evaluation of the various methods that are available both in the macro and micro scale for cell lysis. Various types of cells, the structure of their membranes are discussed initially. Then, various methods that are currently used to lyse cells in the macroscale are discussed and compared. Subsequently, popular methods for micro scale cell lysis and different microfluidic devices used are detailed with their advantages and disadvantages. Finally, a comparison of different techniques used in microfluidics platform has been presented which will be helpful to select method for a particular application.

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“…Kremer et al [ 71 ] lysed cells using an opto-electrical setup. They were able to lyse cells selected based on shape of the cell.…”

Section: Microfabricated Platforms For Cell Lysismentioning

confidence: 99%

A Review on Macroscale and Microscale Cell Lysis Methods

2017

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“…S1). As shown, although the force changes as the distance changes, the expected separation (less than a few hundred nanometers23) will have little effect on the forces calculated.…”

Section: Resultsmentioning

confidence: 84%

Manipulating and assembling metallic beads with Optoelectronic Tweezers

Zhang

Juvert

Cooper

et al. 2016

Sci Rep

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Optoelectronic tweezers (OET) or light-patterned dielectrophoresis (DEP) has been developed as a micromanipulation technology for controlling micro- and nano-particles with applications such as cell sorting and studying cell communications. Additionally, the capability of moving small objects accurately and assembling them into arbitrary 2D patterns also makes OET an attractive technology for microfabrication applications. In this work, we demonstrated the use of OET to manipulate conductive silver-coated Poly(methyl methacrylate) (PMMA) microspheres (50 μm diameter) into tailored patterns. It was found that the microspheres could be moved at a max velocity of 3200 μm/s, corresponding to 4.2 nano-newton (10−9 N) DEP force, and also could be positioned with high accuracy via this DEP force. The underlying mechanism for this strong DEP force is shown by our simulations to be caused by a significant increase of the electric field close to the particles, due to the interaction between the field and the silver shells coating the microspheres. The associated increase in electrical gradient causes DEP forces that are much stronger than any previously reported for an OET device, which facilitates manipulation of the metallic microspheres efficiently without compromise in positioning accuracy and is important for applications on electronic component assembling and circuit construction.

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“…Initially, low OET bias (0.2 kV/cm) was used to position individual cells in specified locations, followed by application of high electroporation bias (1.5 kV/cm) to selected cells resulting in the intracellular delivery of the PI dye (Figure e). In addition to electroporation, single cell lysis using OET has also been demonstrated (Kremer et al, ; Witte et al, ). Lastly, a device based on a novel concept, Self‐Locking Optoelectronic Tweezers (SLOT), has shown promising results in scaling up single‐cell manipulation across a significantly larger area (Y. Yang et al, ).…”

Section: Biological Applications Of Oetsmentioning

confidence: 99%

Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring

Jorgolli

Nevill²,

Winters

et al. 2019

Biotech & Bioengineering

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The new and rapid advancement in the complexity of biologics drug discovery has been driven by a deeper understanding of biological systems combined with innovative new therapeutic modalities, paving the way to breakthrough therapies for previously intractable diseases. These exciting times in biomedical innovation require the development of novel technologies to facilitate the sophisticated, multifaceted, high‐paced workflows necessary to support modern large molecule drug discovery. A high‐level aspiration is a true integration of “lab‐on‐a‐chip” methods that vastly miniaturize cellulmical experiments could transform the speed, cost, and success of multiple workstreams in biologics development. Several microscale bioprocess technologies have been established that incrementally address these needs, yet each is inflexibly designed for a very specific process thus limiting an integrated holistic application. A more fully integrated nanoscale approach that incorporates manipulation, culture, analytics, and traceable digital record keeping of thousands of single cells in a relevant nanoenvironment would be a transformative technology capable of keeping pace with today's rapid and complex drug discovery demands. The recent advent of optical manipulation of cells using light‐induced electrokinetics with micro‐ and nanoscale cell culture is poised to revolutionize both fundamental and applied biological research. In this review, we summarize the current state of the art for optical manipulation techniques and discuss emerging biological applications of this technology. In particular, we focus on promising prospects for drug discovery workflows, including antibody discovery, bioassay development, antibody engineering, and cell line development, which are enabled by the automation and industrialization of an integrated optoelectronic single‐cell manipulation and culture platform. Continued development of such platforms will be well positioned to overcome many of the challenges currently associated with fragmented, low‐throughput bioprocess workflows in biopharma and life science research.

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Shape‐Dependent Optoelectronic Cell Lysis

Cited by 21 publications

References 28 publications

A Review on Macroscale and Microscale Cell Lysis Methods

A Review on Macroscale and Microscale Cell Lysis Methods

Manipulating and assembling metallic beads with Optoelectronic Tweezers

Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring

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