Shape‐Dependent Optoelectronic Cell Lysis

Kremer, Clemens; Witte, Christian; Neale, Steven L.; Reboud, Julien; Barrett, Michael P.; Cooper, Jonathan M.

doi:10.1002/ange.201307751

Cited by 12 publications

(11 citation statements)

References 28 publications

(38 reference statements)

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“…In contrast to conventional electrical techniques, where larger cells always lyse preferentially to smaller cells, Kremer et al developed a novel method that enables shape-selectivity in such a way that cells with a different geometry will preferentially lyse from within a mixture of cell types [ 87 ]. To achieve this, the authors utilized the form of the cell to enhance a non-uniformity in the electric field.…”

Section: Cell Lysis Methodsmentioning

confidence: 99%

Review of Microfluidic Methods for Cellular Lysis

et al. 2021

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Cell lysis is a process in which the outer cell membrane is broken to release intracellular constituents in a way that important information about the DNA or RNA of an organism can be obtained. This article is a thorough review of reported methods for the achievement of effective cellular boundaries disintegration, together with their technological peculiarities and instrumental requirements. The different approaches are summarized in six categories: chemical, mechanical, electrical methods, thermal, laser, and other lysis methods. Based on the results derived from each of the investigated reports, we outline the advantages and disadvantages of those techniques. Although the choice of a suitable method is highly dependent on the particular requirements of the specific scientific problem, we conclude with a concise table where the benefits of every approach are compared, based on criteria such as cost, efficiency, and difficulty.

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Section: Cell Lysis Methodsmentioning

confidence: 99%

Review of Microfluidic Methods for Cellular Lysis

et al. 2021

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“…S1 ). As shown, although the force changes as the distance changes, the expected separation (less than a few hundred nanometers 23 ) will have little effect on the forces calculated.…”

Section: Resultsmentioning

confidence: 83%

Manipulating and assembling metallic beads with Optoelectronic Tweezers

Zhang

Juvert

Cooper

et al. 2016

Sci Rep

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Optoelectronic tweezers (OET) or light-patterned dielectrophoresis (DEP) has been developed as a micromanipulation technology for controlling micro- and nano-particles with applications such as cell sorting and studying cell communications. Additionally, the capability of moving small objects accurately and assembling them into arbitrary 2D patterns also makes OET an attractive technology for microfabrication applications. In this work, we demonstrated the use of OET to manipulate conductive silver-coated Poly(methyl methacrylate) (PMMA) microspheres (50 μm diameter) into tailored patterns. It was found that the microspheres could be moved at a max velocity of 3200 μm/s, corresponding to 4.2 nano-newton (10−9 N) DEP force, and also could be positioned with high accuracy via this DEP force. The underlying mechanism for this strong DEP force is shown by our simulations to be caused by a significant increase of the electric field close to the particles, due to the interaction between the field and the silver shells coating the microspheres. The associated increase in electrical gradient causes DEP forces that are much stronger than any previously reported for an OET device, which facilitates manipulation of the metallic microspheres efficiently without compromise in positioning accuracy and is important for applications on electronic component assembling and circuit construction.

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“…An AC bias potential is applied between the two substrates. The OET system can switch between manipulation and electroporation (Valley et al 2009) and electrolysis (Kremer et al 2014) depending on the applied bias potential. Reported cell survival rates are around 91 %, and genome-editing success rates of 73 % have been reported (Kaneko et al 2014).…”

Section: Optical Tools In Combination With Orthogonal Techniquesmentioning

confidence: 99%

“…RBC Red blood cell, WBC white blood cell. Reproduced in part from Kremer et al (2014) with permission from John Wiley and Sons trap, where the IR laser was used to bring two cells together and UV pulses were then used to cut the common wall between them to allow fusion (Steubing et al 1991). Alternatively, a laser can be used as a scalpel to isolate individual cells from their binding matrix, as has been demonstrated in symbiotic efficiency studies, such as in the study of Leitz et al (2003) with the nitrogen-fixing soil bacterium Frankia and woody dicotyledonous plants.…”

Section: Interactions Between Traps and Cellsmultipurpose Toolsmentioning

confidence: 99%

Optically-controlled platforms for transfection and single- and sub-cellular surgery

2015

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Improving the resolution of biological research to the single-cell or sub-cellular level is of critical importance in a wide variety of processes and disease conditions. Most obvious are those linked to aging and cancer, many of which are dependent upon stochastic processes where individual, unpredictable failures or mutations in individual cells can lead to serious downstream conditions across the whole organism. The traditional tools of biochemistry struggle to observe such processes: the vast majority are based upon ensemble approaches analysing the properties of bulk populations, which means that details of individual constituents is lost. What are required, then, are tools with the precision and resolution to probe and dissect cells at the single-micron scale: the scale of the individual organelles and structures that control their function. In this review, we highlight the use of highly-focused laser beams to create systems which provide precise control and specificity at the single-cell or even single-micron level. The intense focal points generated can directly interact with cells and cell membranes, which in conjunction with related modalities such as optical trapping provide a broad platform for the development of single-cell and sub-cellular surgery approaches. These highly tuneable tools have been demonstrated to deliver or remove material from cells of interest, and they can simultaneously excite fluorescent probes for imaging purposes or plasmonic structures for very local heating. We discuss both the history and recent applications of the field, highlighting the key findings and developments over the last 40 years of biophotonics research.

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Shape‐Dependent Optoelectronic Cell Lysis

Cited by 12 publications

References 28 publications

Review of Microfluidic Methods for Cellular Lysis

Review of Microfluidic Methods for Cellular Lysis

Manipulating and assembling metallic beads with Optoelectronic Tweezers

Optically-controlled platforms for transfection and single- and sub-cellular surgery

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