SGK1 is essential for meiotic resumption in mammalian oocytes

Llano, Edgar Del; Iyyappan, Rajan; Aleshkina, Daria; Mašek, Tomáš; Dvoran, Michal; Jiang, Zongliang; Pospíšek, Martin; Kubelka, Michal; Šušor, Andrej

doi:10.1016/j.ejcb.2022.151210

Cited by 8 publications

(9 citation statements)

References 47 publications

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“…Bright-field time-lapse imaging was performed on an inverted microscope Leica DMI 6000B (Leica Microsystems, Germany) in a controlled environment (37°C, 5% CO 2 in air) (del Llano et al, 2022). Oocytes/embryos were placed in a 10 µl drop of culture medium covered with oil (oocytes: Mineral oil, Merck, M5310; embryos: OVOIL™, Vitrolife, 10029).…”

Section: Methodsmentioning

confidence: 99%

CHK1-CDC25A-CDK1 regulate cell cycle progression in early mouse embryos to protect genome integrity

Knoblochová

Duricek

Vaškovičová

et al. 2022

Preprint

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After fertilization, remodeling of the oocyte and sperm genomes is essential to convert these highly differentiated non-dividing transcriptionally quiescent cells into early cleavage-stage transcriptionally active totipotent blastomeres. This developmental transition is accompanied by cell cycle adaptation such as lengthening or shortening of the gap phases G1 and G2. However, regulation of these cell cycle changes is poorly understood, especially in mammals. Checkpoint kinase 1 (CHK1) is a protein kinase that regulates cell cycle progression in somatic cells. Here, we show that CHK1 regulates cell cycle progression in early mouse embryos by restraining CDK1 kinase activity due to CDC25A phosphatase degradation. CHK1 kinase also ensures the long G2 phase needed for genome activation and reprogramming gene expression in 2-cell stage mouse embryos. Last,Chk1depletion leads to DNA damage and chromosome segregation errors that result in aneuploidy and infertility.

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Section: Methodsmentioning

confidence: 99%

CHK1-CDC25A-CDK1 regulate cell cycle progression in early mouse embryos to protect genome integrity

Knoblochová

Duricek

Vaškovičová

et al. 2022

Preprint

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“…166,167 Contrary to PKA, SGK1 and AURKA have been demonstrated to be responsible for the activation of MPF by phosphorylating and activating CDC25. 168,169 Besides, ERK1/2-mediated phosphorylation is also essential for oocytes oocyte meiotic maturation and MZT. 170 ERK1/2 can promote the phosphorylation and activation of CPEB1, which triggers the polyadenylation and translation activation of the maternal mRNAs containing CPE in oocytes.…”

Section: Protein Phosphorylationmentioning

confidence: 99%

Epigenetic reprogramming during the maternal‐to‐zygotic transition

Chen

Wang

Guo

et al. 2023

MedComm

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After fertilization, sperm and oocyte fused and gave rise to a zygote which is the beginning of a new life. Then the embryonic development is monitored and regulated precisely from the transition of oocyte to the embryo at the early stage of embryogenesis, and this process is termed maternal‐to‐zygotic transition (MZT). MZT involves two major events that are maternal components degradation and zygotic genome activation. The epigenetic reprogramming plays crucial roles in regulating the process of MZT and supervising the normal development of early development of embryos. In recent years, benefited from the rapid development of low‐input epigenome profiling technologies, new epigenetic modifications are found to be reprogrammed dramatically and may play different roles during MZT whose dysregulation will cause an abnormal development of embryos even abortion at various stages. In this review, we summarized and discussed the important novel findings on epigenetic reprogramming and the underlying molecular mechanisms regulating MZT in mammalian embryos. Our work provided comprehensive and detailed references for the in deep understanding of epigenetic regulatory network in this key biological process and also shed light on the critical roles for epigenetic reprogramming on embryonic failure during artificial reproductive technology and nature fertilization.

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“…Along with PKA inactivation, other kinases that regulate oocyte maturation become active in parallel pathways. For example, serum‐glucocorticoid kinase (SGK1) has been shown to phosphorylate and activate CDC25B phosphatase, which in turn removes the inhibitory phosphorylation of CDK1 and activates MPF (del Llano et al, 2022). However, mice lacking either sgk1 alone or both sgk1 and sgk3 do not display any functional abnormalities in the ovary or infertility (Grahammer et al, 2006; Wulff et al, 2002), questioning the involvement of SGK1 during physiological oocyte maturation.…”

Section: Oocyte Maturation and Involvement Of Erk Signaling Pathway I...mentioning

confidence: 99%

“…Along with PKA inactivation, other kinases that regulate oocyte maturation become active in parallel pathways. For example, serumglucocorticoid kinase (SGK1) has been shown to phosphorylate and activate CDC25B phosphatase, which in turn removes the inhibitory phosphorylation of CDK1 and activates MPF (del Llano et al, 2022).…”

Section: Resumption Of Meiosis: Releasing the Arrestmentioning

confidence: 99%

Regulation of oocyte maturation: Role of conserved ERK signaling

Das

Arur

2022

Molecular Reproduction Devel

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During oogenesis, oocytes arrest at meiotic prophase I to acquire competencies for resuming meiosis, fertilization, and early embryonic development. Following this arrested period, oocytes resume meiosis in response to species‐specific hormones, a process known as oocyte maturation, that precedes ovulation and fertilization. Involvement of endocrine and autocrine/paracrine factors and signaling events during maintenance of prophase I arrest, and resumption of meiosis is an area of active research. Studies in vertebrate and invertebrate model organisms have delineated the molecular determinants and signaling pathways that regulate oocyte maturation. Cell cycle regulators, such as cyclin‐dependent kinase (CDK1), polo‐like kinase (PLK1), Wee1/Myt1 kinase, and the phosphatase CDC25 play conserved roles during meiotic resumption. Extracellular signal‐regulated kinase (ERK), on the other hand, while activated during oocyte maturation in all species, regulates both species‐specific, as well as conserved events among different organisms. In this review, we synthesize the general signaling mechanisms and focus on conserved and distinct functions of ERK signaling pathway during oocyte maturation in mammals, non‐mammalian vertebrates, and invertebrates such as Drosophila and Caenorhabditis elegans.

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SGK1 is essential for meiotic resumption in mammalian oocytes

Cited by 8 publications

References 47 publications

CHK1-CDC25A-CDK1 regulate cell cycle progression in early mouse embryos to protect genome integrity

CHK1-CDC25A-CDK1 regulate cell cycle progression in early mouse embryos to protect genome integrity

Epigenetic reprogramming during the maternal‐to‐zygotic transition

Regulation of oocyte maturation: Role of conserved ERK signaling

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