SFE-GC with Quantitative Transfer of the Extraction Effluent to a Megabore Capillary Column

Abstract

Supercritical fluid extraction was directly coupled with gas chromatography such that the entire extraction effluent was directed onto the GC column. A sealed connection between the restrictor and the column ensured that no loss of analyte could occur. The elevated pressure generated in the column itself resulted in a reduced flow rate through the column, making efficient trapping possible. Metal-jacketed capillary columns were used as they can tolerate the elevated pressures required. In addition to maximizing sensitivity, very practical extraction parameters were possible. For a 25-min extraction, with a 2.2 mL/min flow rate, and the column at 40 degrees C, analytes as volatile as C-14 were trapped and separated. Trapping efficiency improved further with shorter extraction times, mild cooling of the column, or lower flow rates.

Optimizing Textile Dyeing Wastewater Irrigation through Physiochemical Attributes of Tomato, Plant Nutrient Use Efficiency and Pollution Load Index of Irrigated Soil

“…Bottles were thoroughly washed with 1M HCl and rinsed several times with de-ionized water before sample collection. Sampling was carried out using the grab method except mixed effluent [25]. Among the seven studied TDW, six different stages of effluents were collected from the processing steps of dyeing whereas mixed effluent sample was taken from equalization tank those were discharged before purifying at ETP.…”