SF/PVP nanofiber wound dressings loaded with phlorizin: preparation, characterization, in vivo and in vitro evaluation

Sun, Shuwen; Hao, Mingqian; Ding, Chuanbo; Zhang, Jinping; Ding, Qiteng; Zhang, Yiwen; Zhao, Yingchun; Liu, Wencong

doi:10.1016/j.colsurfb.2022.112692

Cited by 33 publications

(14 citation statements)

References 45 publications

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“…In the model of full-thickness skin wound healing, three groups including control (gauze), A-0, and A-5 were used to demonstrate the potential of AMFs [ 70 , 71 ]. As illustrated in Figure 8 a, the defected skin was significantly different at days 3, 7, 14, and 21 among all groups.…”

Section: Resultsmentioning

confidence: 99%

Actinomycin-X2-Immobilized Silk Fibroin Film with Enhanced Antimicrobial and Wound Healing Activities

Zhou

Xie

et al. 2023

IJMS

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Actinomycin is a family of chromogenic lactone peptides that differ in their peptide portions of the molecule. An antimicrobial peptide, actinomycin X2 (Ac.X2), was produced through the fermentation of a Streptomyces cyaneofuscatus strain. Immobilization of Ac.X2 onto a prepared silk fibroin (SF) film was done through a carbodiimide reaction. The physical properties of immobilized Ac.X2 (antimicrobial films, AMFs) were analyzed by ATR-FTIR, SEM, AFM, and WCA. The findings from an in vitro study showed that AMFs had a more broad-spectrum antibacterial activity against both S. aureus and E. coli compared with free Ac.X2, which showed no apparent strong effect against E. coli. These AMFs showed a suitable degradation rate, good hemocompatibility, and reduced cytotoxicity in the biocompatibility assay. The results of in vivo bacterially infected wound healing experiments indicated that wound inflammation was prevented by AMFs, which promoted wound repair and improved the wound microenvironment. This study revealed that Ac.X2 transformation is a potential candidate for skin wound healing.

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Section: Resultsmentioning

confidence: 99%

Actinomycin-X2-Immobilized Silk Fibroin Film with Enhanced Antimicrobial and Wound Healing Activities

Zhou

Xie

et al. 2023

IJMS

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“…And HaCaT cells culture and cell viability assay (MTT method) were performed as previously described. 29 …”

Section: Methodsmentioning

confidence: 99%

Chitosan/Sodium Alginate/Velvet Antler Blood Peptides Hydrogel Promotes Diabetic Wound Healing via Regulating Angiogenesis, Inflammatory Response and Skin Flora

Hao

Ding

Sun

et al. 2022

JIR

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Background Diabetic ulcer remains a clinical challenge due to impaired angiogenesis and persistent inflammation, requiring new alternative therapies to promote tissue regeneration. Purpose In this study, chitosan/sodium alginate/velvet antler blood peptides (CS/SA/VBPs) hydrogel (CAVBPH) was fabricated and used in the treatment of skin wounds in type 2 diabetes mellitus (T2D) for the first time. Methods VBPs were prepared by hydrolysis and ultrafiltration, and their sequences were identified using LC-MS/MS. The CAVBPH was further fabricated and characterized. A mouse model of T2D was induced by a high-sugar and high-fat diet (HSFD) and streptozotocin (STZ) injection. CAVBPH was applied topically to T2D wounds, and its effects on skin repair and potential biological mechanisms were analyzed by appearance observation, histopathological staining, bioinformatics analysis, Western blot, and 16S rRNA sequencing. Results VBPs had numerous short-chain active peptides, excellent antioxidant activity, and a low hemolysis rate. CAVBPH exhibited desirable biochemical properties and participated in the diabetic wound healing process by promoting cell proliferation (PCNA and α-SMA) and angiogenesis (capillaries and CD31) and alleviating inflammation (CD68). Mechanistically, the therapeutic effect of CAVBPH on chronic wounds might rely on activating the PI3K/AKT/mTOR/HIF-1α/VEGFA pathway and reversing the expression of inflammatory cytokines TNF-α and IL-1β. The results of 16S rRNA sequencing indicated that T2D significantly altered the diversity and structure of skin flora at the wound site. CAVBPH treatment elevated the relative abundance of beneficial microbes (e.g., Corynebacterium_1 and Lactobacillus ) and reversed the structural imbalance of skin microbiota. Conclusion These results indicate that CAVBPH is a promising wound dressing, and its repair effect on diabetic wounds by regulating angiogenesis, inflammatory response, and skin flora may depend on the rich small peptides in VBPs.

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“…Appropriate mechanical properties and exibility are preconditions for wound dressings. 35,36 Typical stress-strain curves were similar during Ag@PLA, Zn@PLA, and Ag/Zn@PLA's stretching (Fig. 2d).…”

Section: Physical and Chemical Characterization Of Ag/zn@plamentioning

confidence: 77%

Electroactive nanofibrous membrane with temperature monitoring for wound healing

Yang

Wang

et al. 2023

RSC Adv.

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SF/PVP nanofiber wound dressings loaded with phlorizin: preparation, characterization, in vivo and in vitro evaluation

Cited by 33 publications

References 45 publications

Actinomycin-X2-Immobilized Silk Fibroin Film with Enhanced Antimicrobial and Wound Healing Activities

Actinomycin-X2-Immobilized Silk Fibroin Film with Enhanced Antimicrobial and Wound Healing Activities

Chitosan/Sodium Alginate/Velvet Antler Blood Peptides Hydrogel Promotes Diabetic Wound Healing via Regulating Angiogenesis, Inflammatory Response and Skin Flora

Electroactive nanofibrous membrane with temperature monitoring for wound healing

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