Sexually dimorphic expression in developing and adult gonads shows an important role of gonadal soma-derived factor during sex differentiation in olive flounder ( Paralichthys olivaceus )

Liu, Yuezhong; Zhang, Wei; Du, Xinxin; Zhao, Jun; Liu, Xiaobing; Li, Xiaojing; Zhang, Quanqi; Wang, Xubo

doi:10.1016/j.cbpb.2017.05.003

Cited by 21 publications

(18 citation statements)

References 26 publications

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“…In this study we found that gsdf is highly expressed in females and males, with specific transcript localization in granulosa and Sertoli cells, respectively. Likewise, expression of gsdf in the same cell types has been observed in several fish species including rainbow trout (Oncorhynchus mykiss), medaka (Oryzias latipes), zebrafish, Nile tilapia (Oreochromis niloticus), Olive flounder (Paralichthys olivaceus), Spotted scat (Scatophagus argus) and Japanese flounder (Paralichthys olivaceus) [24,[42][43][44][45][46][47][48]. Transcripts of gsdf have also been detected within oocytes in Olive flounder, and within spermatogonia and spermatids in Chinese tongue sole (Cynoglossus semilaevis) [46,49].…”

Section: Gsdfmentioning

confidence: 83%

“…Likewise, expression of gsdf in the same cell types has been observed in several fish species including rainbow trout (Oncorhynchus mykiss), medaka (Oryzias latipes), zebrafish, Nile tilapia (Oreochromis niloticus), Olive flounder (Paralichthys olivaceus), Spotted scat (Scatophagus argus) and Japanese flounder (Paralichthys olivaceus) [24,[42][43][44][45][46][47][48]. Transcripts of gsdf have also been detected within oocytes in Olive flounder, and within spermatogonia and spermatids in Chinese tongue sole (Cynoglossus semilaevis) [46,49]. Although the function(s) of gsdf in male fish seems strongly associated with germ cells, it may differ between species since this gene has been linked to several processes such as proliferation of primordial germ cells and spermatogonia in rainbow trout, sex determination in Oryzias luzonensis, sex differentiation in Chinese tounge sole, and testis differentiation in medaka and Nile tilapia [24,[49][50][51][52][53].…”

Section: Gsdfmentioning

confidence: 83%

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Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

et al. 2020

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Background: Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently sterile salmon was produced by knocking out the germ cell-specific dead end (dnd). Several approaches may be applied to inhibit Dnd function, including gene knockout, knockdown or immunization. Since it is challenging to develop a successful treatment against a gene product already existing in the body, alternative targets are being explored. Germ cells are surrounded by, and dependent on, gonadal somatic cells. Targeting genes essential for the survival of gonadal somatic cells may be good alternative targets for sterility treatments. Our aim was to identify and characterize novel germ cell and gonadal somatic factors in Atlantic salmon. Results: We have for the first time analysed RNA-sequencing data from germ cell-free (GCF)/dnd knockout and wild type (WT) salmon testis and searched for genes preferentially expressed in either germ cells or gonadal somatic cells. To exclude genes with extra-gonadal expression, our dataset was merged with available multi-tissue transcriptome data. We identified 389 gonad specific genes, of which 194 were preferentially expressed within germ cells, and 11 were confined to gonadal somatic cells. Interestingly, 5 of the 11 gonadal somatic transcripts represented genes encoding secreted TGF-β factors; gsdf, inha, nodal and two bmp6-like genes, all representative vaccine targets. Of these, gsdf and inha had the highest transcript levels. Expression of gsdf and inha was further confirmed to be gonad specific, and their spatial expression was restricted to granulosa and Sertoli cells of the ovary and testis, respectively. Finally, we show that inha expression increases with puberty in both ovary and testis tissue, while gsdf expression does not change or decreases during puberty in ovary and testis tissue, respectively. Conclusions: This study contributes with transcriptome data on salmon testis tissue with and without germ cells. We provide a list of novel and known germ cell-and gonad somatic specific transcripts, and show that the expression of two highly active gonadal somatic secreted TGF-β factors, gsdf and inha, are located within granulosa and Sertoli cells.

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Section: Gsdfmentioning

confidence: 83%

Section: Gsdfmentioning

confidence: 83%

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

et al. 2020

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“…It has been reported that Japanese flounder amh is sexually dimorphic during sex gonadal differentiation [ 19 ]. For the lack of genetic gender marker in Japanese flounder, we referenced the expression of amh to distinguish the genetic male and female individuals [ 20 ]. The exact expression of amh , Posox9a and Posox9b in gonads during the early development stage (30 to 100 dah) were analyzed by qRT-PCR.…”

Section: Resultsmentioning

confidence: 99%

“…It has been found via histological observation that the ovarian and testicular differentiation in P. olivaceus juveniles initiated when total length (TL) reached 30 and 37 mm [ 26 , 27 ], thus juveniles of 60 dah (32.7 mm) were selected as the initiation of gonadal differentiation in this study [ 20 ]. At this critical point of 60 dah, Posox9a and Posox9a increased sharply compared with 30 dah, and from then on they both showed obvious sexual dimorphic expression, denoting their important roles in Japanese flounder male sex differentiation.…”

Section: Discussionmentioning

confidence: 99%

Roles of Two Sox9 Genes during Gonadal Development in Japanese Flounder: Sex Differentiation, Spermatogenesis and Gonadal Function Maintenance

Wang

et al. 2018

IJMS

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The transcription factor sox9 has been implicated in cartilage formation and testis determination in mammals. Here, two duplicates of sox9 were found in Japanese flounder (Paralichthys olivaceus) named Posox9a and Posox9b, respectively. Phylogenetic and gene structure analyses revealed that Posox9a and Posox9b were homologous to that of teleosts and tetrapods. Quantitative real-time polymerase chain reaction (qRT-PCR) showed that both Posox9a and Posox9b expressed higher in testis than in ovary of adult tissues. The in situ hybridization (ISH) analysis of gonads showed that Posox9a and Posox9b mRNA were both detected in oocytes, Sertoli cells and spermatocytes. During sex differentiation, the expression of Posox9a exhibited obvious sexual dimorphic expression from 60 days after hatch (dah) with higher expression in male preferred individuals than female preferred individuals and increased gradually from 30 to 100 dah. A similar pattern was detected in Posox9b expression. After injection of androgen (17α-methyltestosterone) of different concentrations, the expression level of Posox9b increased significantly, whereas Posox9a did not change obviously. These results indicated that the two sox9 genes of Japanese flounder had converse functions in sex differentiation, whereas their differences in 17α-methyltestosterone administration were obvious and worthwhile for exploring evolutionary and adaptive significance. This study provided a foundation for further exploration of the roles of Posox9 genes during the sex determination and differentiation, spermatogenesis and gonadal function maintenance of Japanese flounder.

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“…As a fish specific TGF-β family gene, gsdf is well known for its function in male sex determination and differentiation [9,12,[22][23][24]. In the present Cys 146 and a cysteine residue of another molecule [20].…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis Evolution History of Gonadal Soma-derived Factor (gsdf) in Black Rockfish, Sebastes schlegelii

Wang

2019

AJAAR

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As a teleost- and gonad-specific growth factor, gsdf has been indicated to play an important role in sex differentiation and determination. In this study, the complete open reading frame (ORF) of gsdf was isolated from black rockfish, Sebastes schlegelii. Bioinformatics analysis showed that there is a conserved transforming growth factor-β (TGF-β) domain located on the C-terminus of Gsdf. Multiple sequence alignments revealed that fish gsdf were highly conserved in TGF-β domain which suggested their functional conservatism. Synteny analysis provided evidence for the hypothesis that gsdf was originated from fish-specific genome duplication (FSGD). To further explore its function, the expression pattern was examined based on the RNA-seq data and the result showed that significantly sexually dimorphic expression existing between male and female individuals. These results suggested that gsdf might play an important role in maintenance of male characteristics in Sebastes schlegelii.

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Sexually dimorphic expression in developing and adult gonads shows an important role of gonadal soma-derived factor during sex differentiation in olive flounder ( Paralichthys olivaceus )

Cited by 21 publications

References 26 publications

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

Roles of Two Sox9 Genes during Gonadal Development in Japanese Flounder: Sex Differentiation, Spermatogenesis and Gonadal Function Maintenance

Functional Analysis Evolution History of Gonadal Soma-derived Factor (gsdf) in Black Rockfish, Sebastes schlegelii

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