Sexual Reproduction in the White Pine Weevil (Pissodes strobi [Peck] [Coleoptera: Curculionidae]): Implications for Population Genetic Diversity

Lewis, Kornelia G.; Liewlaksaneeyanawin, Cherdsak; Alfaro, R. I.; Ritland, Carol; Ritland, Kermit; El‐Kassaby, Yousry A.

doi:10.1093/jhered/93.3.165

Cited by 4 publications

(2 citation statements)

References 11 publications

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“…This approach has been helpful to establish the paternity of guarding males in species where no information on mating histories of both males and females are known. Using molecular markers, it has been shown how the females in some nonparthenogenetic insect species such as white pine weevil, an important forest pest, carry sperms of more than one male from one season to the next (Lewis et al . 2002).…”

Section: Major Applications Of Molecular Markers In Studying Insect Ementioning

confidence: 99%

Molecular marker systems in insects: current trends and future avenues

2006

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Insects comprise the largest species composition in the entire animal kingdom and possess a vast undiscovered genetic diversity and gene pool that can be better explored using molecular marker techniques. Current trends of application of DNA marker techniques in diverse domains of insect ecological studies show that mitochondrial DNA (mtDNA), microsatellites, random amplified polymorphic DNA (RAPD), expressed sequence tags (EST) and amplified fragment length polymorphism (AFLP) markers have contributed significantly for progresses towards understanding genetic basis of insect diversity and for mapping medically and agriculturally important genes and quantitative trait loci in insect pests. Apart from these popular marker systems, other novel approaches including transposon display, sequence-specific amplification polymorphism (S-SAP), repeat-associated polymerase chain reaction (PCR) markers have been identified as alternate marker systems in insect studies. Besides, whole genome microarray and single nucleotide polymorphism (SNP) assays are becoming more popular to screen genome-wide polymorphisms in fast and cost effective manner. However, use of such methodologies has not gained widespread popularity in entomological studies. The current study highlights the recent trends of applications of molecular markers in insect studies and explores the technological advancements in molecular marker tools and modern high throughput genotyping methodologies that may be applied in entomological researches for better understanding of insect ecology at molecular level.

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Section: Major Applications Of Molecular Markers In Studying Insect Ementioning

confidence: 99%

Molecular marker systems in insects: current trends and future avenues

2006

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“…In this sense, molecular techniques are increasingly being used to study the population structure and species composition and to recognize complex of cryptic species (Gebeyehu andWingfield 2003, Zhang et al 2007). Indeed, molecular studies on Pissodes species have been undertaken to solve taxonomic problems, to explore phylogenetics, and to study aspects of population genetics within the P. strobi complex (Boyce et al 1994, Langor and Sperling 1997, Lewis et al 2002, Laffin et al 2004 or within Pissodes validirostris (Gyllenhal) cone pine weevil (G.R., personal communication).…”

Section: Introductionmentioning

confidence: 99%

Introduction and Establishment ofPissodes castaneus(Coleoptera: Curculionidae) in the Andean Patagonia of Argentina

Pereyra¹,

Gómez²,

Manna³

et al. 2015

J Econ Entomol

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The pine weevils that occur in plantations of Pinus spp. in Andean Patagonia of Argentina belong to the species Pissodes castaneus (De Geer), a Eurasian endemic species, according to the identification based on molecular and morphological characters. Sequences of the mitochondrial Cytochrome oxidase subunit I and nuclear genes (28 S rDNA and ITS2) were obtained for individuals of 13 afforestations, covering the entire distribution area of the established populations in the Andean Patagonia of Argentina. Sequence comparison with representative species of the genus (European, North American, and Chinese species) shows that Patagonian specimens are conspecific to those of P. castaneus sequenced from Europe. Phylogenetic analyses indicate that all terminals from Patagonia form a monophyletic unit without evident subclades, eliminating the possibility of existence of more than one species of Pissodes Germar in this area, including cryptic ones. Moreover, the very low genetic divergence between the Patagonian populations suggests that it is plausible that P. castaneus was introduced into Patagonia from just one location. Mitochondrial DNA analysis shows that Patagonian terminals group together with a French haplotype and are clearly separated from other P. castaneus individuals represented in our sample, and reveal that established populations in Andean Patagonia originated via a limited introduction.

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Conifer defense against insects: Proteome analysis of Sitka spruce (Picea sitchensis) bark induced by mechanical wounding or feeding by white pine weevils (Pissodes strobi)

et al. 2007

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Feeding insects can have major ecological and economic impacts on both natural and planted forests. Understanding the molecular and biochemical mechanisms by which conifers defend themselves from insect pests is a major goal of ongoing research in forest health genomics. In previous work, we demonstrated a complex system of anatomical, chemical, and transcriptome responses in Sitka spruce (Picea sitchensis) upon feeding by the economically significant insect pest, the white pine weevil (Pissodes strobi). In this study, changes to the proteome of Sitka spruce bark tissue were examined subsequent to feeding by white pine weevils or mechanical wounding. 2-D PAGE and high-throughput MS/MS were used to examine induced changes in protein abundance and protein modification. Significant changes were observed as early as 2 h following the onset of insect feeding. Among the insect-induced proteins are a series of related small heat shock proteins, other stress response proteins, proteins involved in secondary metabolism, oxidoreductases, and a novel spruce protein. Comparison of protein expression and cDNA microarray profiles of induced spruce stem tissues reveals the complementary nature of transcriptome and proteome analyses and the need to apply a multifaceted approach to the large-scale analysis of plant defense systems.

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Sexual Reproduction in the White Pine Weevil (Pissodes strobi [Peck] [Coleoptera: Curculionidae]): Implications for Population Genetic Diversity

Cited by 4 publications

References 11 publications

Molecular marker systems in insects: current trends and future avenues

Molecular marker systems in insects: current trends and future avenues

Introduction and Establishment ofPissodes castaneus(Coleoptera: Curculionidae) in the Andean Patagonia of Argentina

Conifer defense against insects: Proteome analysis of Sitka spruce (Picea sitchensis) bark induced by mechanical wounding or feeding by white pine weevils (Pissodes strobi)

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