Sexual reproduction and cyst formation in the freshwater dinoflagellate Peridinium cunningtonii.

Sako, Yoshihiko; Ishida, Yūzaburō; Nishijima, Toshitaka; Hata, Yoshihiko

doi:10.2331/suisan.50.743

Cited by 36 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increase in cell count in low N and low total nutrient media could be attributed to the carry-over effect of the previous culture inoculum. Nutrient stress has previously been suggested to significantly elevate temporary cyst production (Olli, 2004) and could influence sexuality or fusion of gametes, triggering hypnocyst/resting cyst formation in many species of dinoflagellates (Sako et al, 1984(Sako et al, , 1985Anderson et al, 1984;Olli, 2004;Smith & Persson, 2005;Shikata et al, 2008). Though several investigations report that temperature-and nutrient-stress can induce sexuality and resting cyst formation (e.g.…”

Section: Discussionmentioning

confidence: 99%

Development, morphological characteristics and viability of temporary cysts ofPyrodinium bahamensevar.compressum(Dinophyceae)in vitro

Onda¹,

Lluisma²,

Azanza³

2014

European Journal of Phycology

View full text Add to dashboard Cite

Pellicle or temporary cysts of Pyrodinium bahamense var. compressum (Böhm) Steidinger, Tester & F.J.R. Taylor and their role in bloom dynamics have not yet been adequately characterized and understood. We investigated the role of temperature-and nutrient-mediated stress as factors that could induce pellicle formation in batch cultures. Cellular features and their implications for temporary cyst viability were examined using confocal laser scanning microscopy (CLSM). Our data suggest that temperature change is one of the key factors influencing pellicle formation, preserving viability at low temperature (i.e. 13°C). Hypnocysts (resting cysts) were not observed. During pellicle formation, motile cells generally undergo ecdysis, extrusion of cytoplasmic materials and bacteria, compaction of the nucleus and non-motility. The outermost covering of the temporary cysts shows red autofluorescence and it contains lower concentrations of chlorophyll (chl) a and no detectable chl c. The nuclear region is surrounded by transitional red bodies and other unidentified cellular structures. Temporary cysts can immediately revert back to the motile state upon exposure to optimum conditions. This is accompanied by the expansion of the nuclear region, regeneration of the chloroplasts and enlargement of the cell. Developmental changes during reversal of temporary cysts to motile forms were also observed to cause breaks in the cell covering that could serve as sites for bacterial entry. Though observed in vitro, such behaviour may also be occurring in nature especially as a response to drastic short-lived environmental changes. This is the first detailed report on the characteristics of temporary cysts of P. bahamense var. compressum.

show abstract

Section: Discussionmentioning

confidence: 99%

Development, morphological characteristics and viability of temporary cysts ofPyrodinium bahamensevar.compressum(Dinophyceae)in vitro

Onda¹,

Lluisma²,

Azanza³

2014

European Journal of Phycology

View full text Add to dashboard Cite

show abstract

“…Although the sexual reproduction and hypnozygote forma tion in several freshwater Peridinium spp. have already observed under light and electron micro scopes, [1][2][3][4]8,9) the quantitative determination of their excystment has been scarcely studied. Therefore, it is not so easy to compare the ex cystment of P. cunningtonii with that of the others species systematically and quantitatively.…”

Section: Discussionmentioning

confidence: 99%

Excystment in the freshwater dinoflagellate Peridinium cunningtonii.

Sako¹,

Ishida²,

Kadota³

et al. 1985

NIPPON SUISAN GAKKAISHI

Self Cite

View full text Add to dashboard Cite

“…For examples, P. cinctum in Lake Kinneret (Eren, 1969), P. cunningtonii and P. penardii in Nagase Reservoir (Hata, 1982), and P. bipes in Kazaya Reservoir (Watanabe, 1982) and in Ananai Reservoir (Hata, 1982). For P. cunningtonii inoculated in nitrogen and phosphorus-deficient medium the sexual life cycle developed in the following 5 phases: i) gamete formation; ii) fusion; iii) planozygote; iv) hypnozygote (cyst); and v) excystment (Sako et al, 1984). It was suggested that the excystment of overwintering cysts of P. cunningtonii provided a seed population for the bloom of this dinoflagellate in the reservoir (Sako et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

“…Of these, seven are members of the genus Peridinium (Pfiester, 1975(Pfiester, , 1976(Pfiester, , 1977Skvarla, 1979, 1980;Sako et al, 1984Sako et al, , 1985. In the first scientific expedition "Studies on Distribution, Adaptation and Evolution of Microorganisms in Nepal Himalayas" in November-December 1982, three subtropical lakes in the Pokhara Valley were surveyed to compare the structure and function of phytoplankton communities in the lakes on the basis of primary production and species diversity (Nakanishi et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Life Cycle of <i>Peridinium</i> sp. B3 (Dinophyceae) Isolated from Lake Begnas, Nepal

Sako

Nakanishi

Konda

et al. 1986

Bulletin of Japanese Society of Microbial Ecology

Self Cite

View full text Add to dashboard Cite

Abstract:Encystment and excystment in the life cycle of a dinoflagellate Peridinium sp. B3, which was isolated from Lake Begnas in the Pokhara Valley, Nepal, were investigated by use of a clonal population.The maximum cell concentration was 3103 vegetative cellsml-1 in BL medium after about 4 weeks at 30C.Sexual reproduction of the cells was induced after the cells in late logarithmic phase were inoculated into nitrogen and phosphorous deficient medium, and also was spontaneously induced in BL medium.Sexual reproduction was isogamous and homothallic. The cycle was initiated by gamete formation, rn which gametes were produced by binary asexual divisions of the vegetative cells. Fusing pairs were formed by the mid-ventral union of gametes. The planozygote retained two red bodies and remained motile for about 2 weeks. Two weeks after the planozygote lost motility, the zygotes enlarged and became dark in color. The theca of the planozygote split around the girdle region and the hypnozygote (cyst) was produced.The matured cysts were dormant and remained viable for at least 1 month at 10C in darkness following maturation for 3 months at 30C. The dark-cold treated cysts excysted synchronously when the incubation temperature was raised to 30C. The protoplast emerged from the cyst wall by amoeboid movement. The excysted cell had two red bodies. This cell divided within 24-48h into 2 daughter cells each with a red body.

show abstract

Sexual reproduction and cyst formation in the freshwater dinoflagellate Peridinium cunningtonii.

Cited by 36 publications

References 0 publications

Development, morphological characteristics and viability of temporary cysts ofPyrodinium bahamensevar.compressum(Dinophyceae)in vitro

Development, morphological characteristics and viability of temporary cysts ofPyrodinium bahamensevar.compressum(Dinophyceae)in vitro

Excystment in the freshwater dinoflagellate Peridinium cunningtonii.

Life Cycle of <i>Peridinium</i> sp. B3 (Dinophyceae) Isolated from Lake Begnas, Nepal

Contact Info

Product

Resources

About