Sexual progeny of somatic hybrids between potato andSolanum brevidens: Potential for use in breeding programs

Helgeson, John P.; Haberlach, Geraldine T.; Ehlenfeldt, Mark K.; Hunt, Greg J.; Pohlman, John; Austin, S.

doi:10.1007/bf02849063

Cited by 45 publications

(13 citation statements)

References 15 publications

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“…However, full expression of these traits in the primary somatic hybrids is essential, and the hybrids must be fertile to allow the undesirable traits of the wild species in the hybrids to be removed through backcrossing. There are a few reports by Ehlenfeldt & Helgeson (1987), Helgeson et al (1993) and Jacobsen et al (1993) addressing the potential of using somatic hybrids of S. tuberosum and S. brevidens through backcrossing in breeding programs.…”

Section: Introductionmentioning

confidence: 99%

Identification of somatic hybrids of dihaploid Solanum tuberosum lines and S. brevidens by species specific RAPD patterns and assessment of disease resistance of the hybrids

Rokka¹,

Kankila³

et al. 1994

Euphytica

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Symmetric somatic hybrids were produced by electrofusion of protoplasts of two dihaploid tuber-bearing potato (Solanum tuberosum L.) lines and Solanum brevidens Phil., a diploid non-tuber-bearing wild potato species. A total of 985 plants was obtained. Verification of nuclear hybridity of putative hybrids was based on additive RAPD patterns, general morphological characteristics and chromosome counts. 53 (90%) calli regenerated into plants which were identified as somatic hybrids. Most of the hybrids were aneuploids at the tetraploid (4 x) or hexaploid (6 x ) level. The 20 hybrids tested expressed a high level of resistance to potato virus Y (PVY N) characteristic of the S. brevidens parent. Resistance to late blight (Phytophthora infestans (Mont.) de Bary) varied between hybrids, but was on average better than that of the fusion parents. Resistance of hybrids to bacterial stem rot (Erwinia carotovora subsp, atroseptica (van Hall) Dye) was not superior to that of commercial potato cultivars.

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Section: Introductionmentioning

confidence: 99%

Identification of somatic hybrids of dihaploid Solanum tuberosum lines and S. brevidens by species specific RAPD patterns and assessment of disease resistance of the hybrids

Rokka¹,

Kankila³

et al. 1994

Euphytica

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“…The total chromosome number and the copy numbers of chromosome 8 are indicated. + Somatic hybridization, x sexual hybridization soft rot assays indicated that all the parental plants used in the somatic hybridization and backcrosses, with the exception of the wild species S. brevidens, are susceptible to soft rot (Austin et al 1988;Helgeson et al 1993). Thus the tuber soft rot resistance in the somatic hybrid A206 and its derivative is presumed to be transmitted from S. brevidens.…”

Section: Resultsmentioning

confidence: 93%

“…brevidens is a non-tuber-bearing wild diploid species that shows a high level of resistance to a number of potato diseases (Helgeson et al 1985;1993). A somatic hybrid, A206, between potato and S. brevidens was developed by protoplast fusion (Austin et al 1986).…”

Section: Introductionmentioning

confidence: 99%

Transfer of tuber soft rot and early blight resistances from Solanum brevidens into cultivated potato

et al. 2004

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Tuber soft rot and early blight are serious potato diseases. Development of potato varieties resistant to these diseases has been hindered by the scarcity of resistant germplasm. A diploid wild species, Solanum brevidens, shows significant resistance to both diseases. Numerous potato breeding lines have been developed from a potato- S. brevidens somatic hybrid, A206. A BC(3) clone, C75-5+297, derived from this somatic hybrid as well as its BC(1) and BC(2) parental lines showed resistance to both tuber soft rot and early blight. Clone C75-5+297 has consistently out-yielded common varieties under disease stress. Using both molecular and cytogenetic approaches we demonstrated that a single copy of chromosome 8 from S. brevidens replaced a potato chromosome 8 in C75-5+297. Thus, C75-5+297 represents a potato- S. brevidens chromosome substitution line. Our results suggest that the presence of a single chromosome from S. brevidens may significantly impact the resistance to multiple potato diseases. The high yield potential of C75-5+297 makes it an excellent parent for developing potato varieties with resistances to both tuber soft rot and early blight.

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“…In general, somatic hybrids have a low pollen fertility. Nevertheless, hybrids have been used as female parents (even though the female fertility might be low) in backcrosses with the cultivated species (Ehlenfeldt & Helgeson, 1987 ;Cardi et al ., 1993 ;Helgeson et al ., 1993 ;Pupilli et al ., 1992 ;Fahleson et al ., 1993 ;1994a, 1994bDixelius, unpublished) . Fertility has also in some cases been higher in asymmetric hybrids than in the corresponding symmetric hybrid (Bates, 1990 ;Melzer & O'Connell, 1992) .…”

Section: Discussionmentioning

confidence: 99%

The potential of somatic hybridization in crop breeding

Waara

Glimelius

1995

Euphytica

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In recent years, the rapid development of somatic cell genetics has made possible the transfer of alien genes over wide taxonomic distances by somatic hybridization . In this review, the potential of somatic hybridization in the breeding of crops within the Brassicaceae, Fabaceae, Poaceae and Solanaceae is discussed . It is evident from these studies that many hybrids, either symmetric or asymmetric, which are fertile have the potential to be used as a bridge between the alien species and the crop . Progeny analysis of some hybrid combinations also reveals intergenomic translocations which may lead to the introgression of the alien genes . Furthermore, fusion techniques enable the resynthesis of allopolyploid crops to increase their genetic variability and to restore ploidy level and heterozygosity after breeding at reduced ploidy level in polyploid crops.

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Sexual progeny of somatic hybrids between potato andSolanum brevidens: Potential for use in breeding programs

Cited by 45 publications

References 15 publications

Identification of somatic hybrids of dihaploid Solanum tuberosum lines and S. brevidens by species specific RAPD patterns and assessment of disease resistance of the hybrids

Identification of somatic hybrids of dihaploid Solanum tuberosum lines and S. brevidens by species specific RAPD patterns and assessment of disease resistance of the hybrids

Transfer of tuber soft rot and early blight resistances from Solanum brevidens into cultivated potato

The potential of somatic hybridization in crop breeding

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