Sexual dimorphism in esterified steroid levels in the gastropod Marisa cornuarietis: The effect of xenoandrogenic compounds

Janer, Gemma; Lyssimachou, Angeliki; Bachmann, Jean; Oehlmann, Jörg; Schulte‐Oehlmann, Ulrike; Porte, Cinta

doi:10.1016/j.steroids.2006.01.012

Cited by 60 publications

(25 citation statements)

References 38 publications

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“…The microsomal enzyme that conjugates testosterone to fatty acids has been designated as acyl coenzyme A:testosterone acyltransferase (ATAT). Since these initial observations, this same process for regulating free hormone levels has been documented in other gastropods for both testosterone and 17b-estradiol (Janer et al 2005c(Janer et al , 2006bSantos et al 2005;Sternberg et al 2008a). Collectively, these metabolic studies indicate that gastropods are capable of biosynthesizing and biotransforming the sex steroids that are present in their tissues.…”

Section: Hormones Of the Reproductive Neuro-endocrine Cascadementioning

confidence: 52%

“…Vertebrate-type sex steroids including estrogens (estrone, 17b-estradiol, estriol) and androgens (androstenedione, androsterone, dehydroepiandrosterone) have been definitively identified in gastropods using gas chromatography-mass spectroscopy (GC-MS) (Le Guellec et al 1987). In addition, sex steroids have been quantified via less-specific immunoassays (e.g., radioimmunoassay) in multiple tissues of gastropods (Bose et al 1997;Gooding and LeBlanc 2004;Janer et al 2006b;Kavaliers et al 2000;Le Guellec et al 1987;Sternberg et al 2008a;Takeda 1980). However, the mere detection of sex steroids in a gastropod provides no insight as to whether these biomolecules actually function as hormones in these organisms.…”

Section: Hormones Of the Reproductive Neuro-endocrine Cascadementioning

confidence: 99%

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Environmental-endocrine control of reproductive maturation in gastropods: implications for the mechanism of tributyltin-induced imposex in prosobranchs

et al. 2009

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Prosobranch snails have been afflicted globally by a condition whereby females develop male sex characteristics, most notably a penis. This condition, known as imposex, has been causally associated with the ubiquitous environmental contaminant tributyltin (TBT). Deduction of the mechanism by which TBT causes imposex has been hampered by the lack of understanding of the normal endocrine regulation of reproductive tract recrudescence in these organisms. We have reviewed the relevant literature on the environmental and endocrine factors that regulate reproductive tract recrudescence, sexual differentiation, and reproduction in gastropods. We provide a cohesive model for the environmental-endocrine regulation of reproduction in these organisms, and use this information to deduce a most likely mechanism by which TBT causes imposex. Photoperiod appears to be the predominant environmental cue that regulates reproductive tract recrudescence. Secondary cues include temperature and nutrition which control the timing of breeding and egg laying. Several hormone products of the central and peripheral nervous systems have been identified that contribute to recrudescence, reproductive behaviors, oocyte maturation and egg laying. Retinoic acid signaling via the retinoid X-receptor (RXR) has shown promise to be a major regulator of reproductive tract recrudescence. Furthermore, TBT has been shown to be a high affinity ligand for the RXR and the RXR ligand 9-cis retinoic acid causes imposex. We propose that TBT causes imposex through the inappropriate activation of this signaling pathway. However, uncertainties remain in our understanding of the environmental-endocrine regulation of reproduction in gastropods. Definitive elucidation of the mechanism of action of TBT awaits resolution of these uncertainties.

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Section: Hormones Of the Reproductive Neuro-endocrine Cascadementioning

confidence: 52%

Section: Hormones Of the Reproductive Neuro-endocrine Cascadementioning

confidence: 99%

Environmental-endocrine control of reproductive maturation in gastropods: implications for the mechanism of tributyltin-induced imposex in prosobranchs

et al. 2009

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“…MT did not affect the fecundity measured as number of egg masses produced, but at 100 ng/L, MT exposure triggered degenerative processes of the albumen and prostate glands (Czech et al, 2001). Females of the freshwater ramshorn snail Marisa cornuarietis exposed to 300 ng/L MT had a significantly higher incidence of imposex than controls (Janer et al, 2006). Our results showed that MT did not cause toxic adverse effects on the invertebrate B. glabrata at the exposure time.…”

Section: Discussionmentioning

confidence: 52%

“…In discussing their results, the authors do not refer to any kind of interference of ethanol in these assays. Janer et al (2006) tested the effects of testosterone on reproduction of the freshwater ramshorn snail Marisa cornuarietis. Testosterone was added in absolute ethanol at a final concentration in water of 0.001%, and snails were exposed to these agents for 50, 100, and 150 days.…”

Section: Discussionmentioning

confidence: 99%

Effects of 17α-methyltestosterone on the reproduction of the freshwater snail Biomphalaria glabrata

Rivero-Wendt¹,

Borges²,

Oliveira-Filho³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. 17-α-methyltestosterone (MT) is a synthetic hormone used in fish hatcheries to induce male monosex. Snails hold promise as possible test models to assess chemicals acting on the endocrine system. Biomphalaria glabrata is an aquatic gastropod mollusk (Pulmonata, Planorbidae) that can be easily maintained in aquaria, predisposing the species for use in ecotoxicological testing. This study evaluated the reproductive effects of MT on B. glabrata by examining histological changes and its reproductive performance. Ten snails per group were exposed for 4 weeks to different concentrations of MT (0.01, 0.1, and 1.0 mg/L). The total number of laid eggs, egg mass per group, size of type V oocytes, and production of spermatozoids were determined. Reproduction of B. glabrata was affected by MT. At the lowest concentration (0.01 mg/L), MT caused a statistically significant increase in the number of egg mass per snail compared with controls unexposed to MT. Histopathology analyses showed an increase in the sperm production at the higher MT concentrations of 0.1 and 1.0 mg/L. Chromatographic analyses of water samples showed that MT concentrations rapidly declined within a 96-h period. These results highlight the importance of giving more support to regulatory authorities, since MT is not registered for use on fish hatcheries in many countries around the world. Wastewater from fish farms discharged into aquatic ecosystems should be monitored for MT residues, since its presence could compromise the reproduction of other native snail species.

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“…In aquatic animals, TBT is known to induce the development of male sex characteristics in females, particularly in several gastropods (Hagger et al 2006;Horiguchi 2006;Horiguchi et al 2006;Janer et al 2006;Viglino et al 2006), thus causing sterility. It also makes aquatic animals more vulnerable to infectious diseases (Anderson et al 1996) and has deleterious effects on oyster growth and reproduction (Alzieu 1991;Gagné et al 2003;Inoue et al 2004Inoue et al , 2006aInoue et al , 2006bInoue et al , 2007.…”

Section: Introductionmentioning

confidence: 99%

Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin

Droguet

Devauchelle

Pennec

et al. 2012

Aquat. Living Resour.

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-European Community regulations on chemicals promote alternative methods to test substances presenting potential risks for the environment. In the present work, cultured atrial cells isolated from oyster (Crassostrea gigas) were used as an experimental model to investigate the toxicity of tributyltin (TBT) after short-time exposure at concentrations representative of those that can be measured in seawater, marine sediments and/or bivalves bioaccumulating this pollutant. In vitro and in vivo assays produce values of the same order of magnitude for both animal/cell survival and heart/cardiomyocyte beating rate. The survival rate of whole animals decreased from 10 −6 M TBT after 3 days. For cultured cells, the viability, evaluated using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay, significantly decreased after two days of treatment with 10 −6 M TBT, and after six days with 10 −10 M TBT. The percentage of apoptotic cells, quantified by flow cytometry and YO-PRO r -1 iodide, a nucleic acid stain that only permeates cells that are beginning to undergo apoptosis, increased significantly in these cases. Moreover, intracellular concentration of Ca ++ had increased after 10 min of exposition to 10 −6 M, and could be associated with apoptotic processes. As patch clamp experiments showed that Ca ++ conductance was decreased, intracellular calcium increase could mainly be due to a release from internal stores. The decreases in beating rhythm could be explained by the decrease in adenosine triphosphate (ATP) production revealed by 31 P nuclear magnetic resonance (NMR) spectroscopy and confirmed by the increase of the K ATP channel conductance. The related hyperpolarization and the disturbances of the energetic metabolism were clearly related to the loss of the atrial cell contractility and viability.

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Sexual dimorphism in esterified steroid levels in the gastropod Marisa cornuarietis: The effect of xenoandrogenic compounds

Cited by 60 publications

References 38 publications

Environmental-endocrine control of reproductive maturation in gastropods: implications for the mechanism of tributyltin-induced imposex in prosobranchs

Environmental-endocrine control of reproductive maturation in gastropods: implications for the mechanism of tributyltin-induced imposex in prosobranchs

Effects of 17α-methyltestosterone on the reproduction of the freshwater snail Biomphalaria glabrata

Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin

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