Sexual Development in Ascomycetes Fruit Body Formation of Aspergillus nidulans

Braus, Gerhard H.; Krappmann, Sven; Eckert, Sabine

doi:10.1201/9780203910719.ch9

Cited by 49 publications

(83 citation statements)

References 90 publications

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“…The life cycle is light-dependent. During illumination, primarily green asexual spores (conidia) are formed on conidiophores, whereas the sexual structures are repressed by light and primarily formed in dark and under limited oxygen levels (Adams et al, 1998;Bayram et al, 2010;Braus et al, 2010Braus et al, , 2002Purschwitz et al, 2008). The closed sexual fruiting bodies (cleistothecia) are surrounded by nursing Hülle cells (Sarikaya Bayram et al, 2010) and contain the red ascospores.…”

Section: Introductionmentioning

confidence: 99%

Fungal S-adenosylmethionine synthetase and the control of development and secondary metabolism in Aspergillus nidulans

Gerke

Bayram

Braus

2012

Fungal Genetics and Biology

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a b s t r a c tThe filamentous fungus Aspergillus nidulans carries a single gene for the S-adenosylmethionine (SAM) synthetase SasA, whereas many other organisms possess multiple SAM synthetases. The conserved enzyme catalyzes the reaction of methionine and ATP to the ubiquitous methyl group donor SAM. SAM is the main methyl group donor for methyltransferases to modify DNA, RNA, protein, metabolites, or phospholipid target substrates. We show here that the single A. nidulans SAM synthetase encoding gene sasA is essential. Overexpression of sasA, encoding a predominantly cytoplasmic protein, led to impaired development including only small sterile fruiting bodies which are surrounded by unusually pigmented auxiliary Hülle cells. Hülle cells are the only fungal cell type which does not contain significant amounts of SasA. Sterigmatocystin production is altered when sasA is overexpressed, suggesting defects in coordination of development and secondary metabolism. SasA interacts with various metabolic proteins including methionine or mitochondrial metabolic enzymes as well as proteins involved in fungal morphogenesis. SasA interaction to histone-2B might reflect a putative epigenetic link to gene expression. Our data suggest a distinct role of SasA in coordinating fungal secondary metabolism and development.

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Section: Introductionmentioning

confidence: 99%

Fungal S-adenosylmethionine synthetase and the control of development and secondary metabolism in Aspergillus nidulans

Gerke

Bayram

Braus

2012

Fungal Genetics and Biology

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“…Sexual development in A. nidulans involves the development of two distinct structures: Hü lle cells and cleistothecia (reviewed in Champe et al 1994;Braus et al 2002). It is important to note that, although Hü lle cells are associated with the sexual reproductive cycle, production of Hü lle cells and development of cleistothecia are distinct processes.…”

Section: G203rmentioning

confidence: 99%

Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

Seo¹,

Han²,

2005

Genetics

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Vegetative growth signaling in the filamentous fungus Aspergillus nidulans is primarily mediated by the heterotrimeric G-protein composed of FadA (Ga), SfaD (Gb), and a presumed Gg. Analysis of the A. nidulans genome identified a single gene named gpgA encoding a putative Gg-subunit. The predicted GpgA protein consists of 90 amino acids showing 72% similarity with yeast Ste18p. Deletion (D) of gpgA resulted in restricted vegetative growth and lowered asexual sporulation. Moreover, similar to the DsfaD mutant, the DgpgA mutant was unable to produce sexual fruiting bodies (cleistothecia) in self-fertilization and was severely impaired with cleistothecial development in outcross, indicating that both SfaD and GpgA are required for fruiting body formation. Developmental and morphological defects caused by deletion of flbA encoding an RGS protein negatively controlling FadA-mediated vegetative growth signaling were suppressed by DgpgA, indicating that GpgA functions in FadA-SfaD-mediated vegetative growth signaling. However, deletion of gpgA could not bypass the need for the early developmental activator FluG in asexual sporulation, suggesting that GpgA functions in a separate signaling pathway. We propose that GpgA is the only A. nidulans Gg-subunit and is required for normal vegetative growth as well as proper asexual and sexual developmental progression.

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“…This fungus produces sexual ascospores within closed fruiting bodies in darkness. These cleistothecia consist of several different cell types as Hü lle cells nursing the young fruiting body [31,32]. In light, the sexual cycle is inhibited and the formation of condiophores is favoured, which results in the production of air-borne asexual spores [29 ,30 ,33,34 ,35].…”

Section: Csn and Light Responsementioning

confidence: 99%

Fungal development and the COP9 signalosome

Braus

Irniger

Bayram

2010

Current Opinion in Microbiology

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The conserved COP9 signalosome (CSN) multiprotein complex is located at the interface between cellular signaling, protein modification, life span and the development of multicellular organisms. CSN is required for light-controlled responses in filamentous fungi. This includes the circadian rhythm of Neurospora crassa or the repression of sexual development by light in Aspergillus nidulans. In contrast to plants and animals, CSN is not essential for fungal viability. Therefore fungi are suitable models to study CSN composition, activity and cellular functions and its role in light controlled development.

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Sexual Development in Ascomycetes Fruit Body Formation of Aspergillus nidulans

Cited by 49 publications

References 90 publications

Fungal S-adenosylmethionine synthetase and the control of development and secondary metabolism in Aspergillus nidulans

Fungal S-adenosylmethionine synthetase and the control of development and secondary metabolism in Aspergillus nidulans

Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

Fungal development and the COP9 signalosome

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