Sexual and Asexual Reproduction of Aspergillus nidulans in vivo: Sexuelle und asexuelle Reproduktion von Aspergillus nidulans in vivo

Kuttin, E. S.; Kaplan, William E.; Scholer, H. I.; Burtscher, H.; Köhler, H.

doi:10.1111/j.1439-0507.1985.tb02103.x

Mycoses

1985

DOI: 10.1111/j.1439-0507.1985.tb02103.x

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Sexual and Asexual Reproduction of Aspergillus nidulans in vivo: Sexuelle und asexuelle Reproduktion von Aspergillus nidulans in vivo

E. S. Kuttin

William E. Kaplan

H. I. Scholer

et al.

Abstract: Summary: Both the sexual and asexual fruiting bodies of Aspergillus nidulans were discovered in the lung tissue of a captive rhinoceros, in a biopsy specimen from a human with bronchial stump aspergillosis, and in the guttural pouch of a horse. In addition, a zygomycete with unusual morphology was found in the lungs of the rhinoceros. Zusammenfassung: Es wurden sowohl sexuelle als auch asexuelle Sporen von Aspergillus nidulans im menschlichen Biopsiematerial eines Patienten mit Bronchialstumpfmykose sowie im… Show more

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Cited by 7 publications

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Riboflavin Level Manipulates the Successive Developmental Sequences in Aspergillus nidulans

Zheng

Zhang

et al. 2015

Curr Microbiol

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Auxotrophic markers are useful in fungal genetic analysis. Among the auxotrophic markers, riboB2 is one of the most commonly used markers in many laboratory strains. However, riboB2 mutants in Aspergillus nidulans confer self-sterility and thus are unable to form hybrid cleistothecia by outcross when both parent strains harbor riboB2 auxotrophic marker under the standard protocol. To assess the role of riboflavin during the different developmental stages of A. nidulans, the limited concentrations of riboflavin were monitored. The commonly used dosage of riboflavin (2.5 µg/ml) in the standard medium recipe is enough for hyphal growth and conidiation in the riboflavin auxotrophic riboB2 mutants (enough at 0.02 and 0.5 μg/ml, respectively) in A. nidulans. However, the dosage is not enough to support mature cleistothecium formation. Furthermore, the self-sterile defects in riboB2 mutants on standard medium could be restored by the addition of 25 μg/ml riboflavin, although the required riboflavin concentrations are varied in different genotype strains in A. nidulans. Most importantly, the outcross between riboB2 mutants could also be achieved by the supply of riboflavin in the sexual developmental stage. Our results highlight the potential roles of auxotrophic markers in the development of fungi and improve the efficiency of the genetic analysis in A. nidulans.

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Riboflavin Level Manipulates the Successive Developmental Sequences in Aspergillus nidulans

Zheng

Zhang

et al. 2015

Curr Microbiol

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Emericella nidulansin a maxillary sinus fungal mass

Mitchell

Chaplin²,

Mackenzie³

1987

Med Mycol

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Sexual reproductive stages of fungi are very rarely found within mammalian tissues. We report here coexistence of cleistothecia associated with Emericella nidulans and its conidial state, Aspergillus nidulans, in a fungal mass which developed in a maxillary sinus.A 67-year-old woman complained of pain and discharge following an operation on her maxillary antrum 8 years previously. A clinical diagnosis was made of a cholesteatoma or a foreign body. Two biopsies were obtained, one mucosal and the other consisting of grey membranous tissue and greyish-brown debris. Histology showed the features of chronic inflammation but there was no evidence of malignancy or of a foreign body. The debris consisted almost entirely of dense mycelium within which several cleistothecia were embedded (Fig. 1). These were rounded, 120-130/tm in diameter, containing abundant lenticular ascospores ca. 4-0 × 4-5 #m with conspicuous paired equatorial crests. Cleistothecia were associated with Hiille cells ca. 20/zm in diameter. Confirmation of identity was provided by the presence in other parts of the sectioned fungal mass of short, smooth, sinuous conidiophores and conidia 3.0-3.5 /zm in diameter typical ofAspergillus nidulans (Fig. 2). In the routine histopathological sections stained with haematoxylin and eosin, details of sporing structures were clearly seen. The characteristic brown colour of the conidiophores was also readily visible, although the distinctive red colour of the ascospores of Emericella nidulans (the sexual sporing state) could not be distinguished. The patient was discharged after sinovectomy and was well when seen 2 years later.The literature contains only a few reports of similar findings. La Touche [4] observed cleistothecia and ascospores of A. glaucus and A. nidulans in debris removed from the human ear. Stedham et al. [6] noted the presence of sexual and asexual sporing forms ofA. nidulans in the lungs of captive egrets. Doby & Kombila-Favry [2] described a biopsy specimen from a human maxillary sinus which in histological sections showed cleistothecia surrounded by Hiille cells. Since only A. fumigatus was isolated in culture, the infection was regarded as a mixed one. In a brief note

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Emericella nidulansas an agent of guttural pouch mycosis in a horse

Guillot¹,

Collobert²,

Gueho³

et al. 1997

Med Mycol

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Sexual and Asexual Reproduction of Aspergillus nidulans in vivo: Sexuelle und asexuelle Reproduktion von Aspergillus nidulans in vivo

Cited by 7 publications

References 10 publications

Riboflavin Level Manipulates the Successive Developmental Sequences in Aspergillus nidulans

Riboflavin Level Manipulates the Successive Developmental Sequences in Aspergillus nidulans

Emericella nidulansin a maxillary sinus fungal mass

Emericella nidulansas an agent of guttural pouch mycosis in a horse

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