Sexing errors among museum skins of a sexually monomorphic bird, the Moorhen <i>Gallinula chloropus</i>

Lee, Patricia L. M.; Griffiths, Richard

doi:10.1046/j.1474-919x.2003.00201.x

Cited by 12 publications

(17 citation statements)

References 28 publications

(31 reference statements)

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“…Sexing is based on the detection of a chromosome-specific intron size difference in the sex-linked CHD gene (chromoboxhelicase-DNA-binding), producing amplicons in the region of 300-400 bp (Griffiths et al 1998). This method is likely to perform poorly with non-invasive samples because fragments larger than 300 bp are difficult to amplify consistently (Lee and Griffiths 2003). New primers that produce shorter fragments have been recently designed (Bantock et al 2008).…”

Section: Designing a New Set Of ''Sex Primers'' Specific For Capercaimentioning

confidence: 99%

Improving non-invasive genotyping in capercaillie (Tetrao urogallus): redesigning sexing and microsatellite primers to increase efficiency on faeces samples

Pérez

Vázquez

Quirós

et al. 2011

Conservation Genet Resour

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A set of 14 microsatellite loci were used for amplification of faecal DNA from capercaillie (Tetrao urogallus). New internal primers were designed for each locus and were employed to perform a seminested PCR approach combined with a multiplex preamplification method. The aim of this process was to increase the quality of the multilocus genotypes and to reduce the amount of sample required. Values of allelic dropout and false alleles found after three repetitions were 8.1 and 1.4% respectively, and 52.63% of the samples amplified for a minimum of 12 loci. Additionally, capercaillie specific sex primers have been designed. These primers give short products suitable for degraded faecal sample amplification. New primers resulted in 87.5% of samples being successfully sexed, a value seven times higher than using the original P2/P8 CHD sexing method.

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Section: Designing a New Set Of ''Sex Primers'' Specific For Capercaimentioning

confidence: 99%

Improving non-invasive genotyping in capercaillie (Tetrao urogallus): redesigning sexing and microsatellite primers to increase efficiency on faeces samples

Pérez

Vázquez

Quirós

et al. 2011

Conservation Genet Resour

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“…However, studies on five auklet species, one rail and three shorebird species report within‐sex length variation in the CHDZ locus (Dawson et al. 2001, Lee & Griffiths 2003, Schroeder et al. 2008a, Casey et al.…”

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confidence: 99%

“…However, studies on five auklet species, one rail and three shorebird species report within-sex length variation in the CHDZ locus (Dawson et al 2001, Lee & Griffiths 2003, Schroeder et al 2008a, Casey et al 2009). In some cases, this complicates band interpretation and can lead to incorrect sex assignment (Dawson et al 2001, Robertson & Gemmel 2006.…”

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confidence: 99%

Linking intronic polymorphism on the CHD1‐Z gene with fitness correlates in Black‐tailed Godwits Limosa l. limosa

Schroeder

Kentie

Velde

et al. 2010

Ibis

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We show that variation in an intronic length polymorphism in the CHD1‐Z gene in Black‐tailed Godwits Limosa l. limosa is associated with fitness correlates. This is the second example of the CHDZ‐1 gene being correlated with fitness, a previous study having established that Moorhens Gallinula chloropus carrying the rare Z* allele have reduced survival. In Godwits, however, carriers of the Z* allele (374 bp) fared better than those with the more frequent Z allele (378 bp) with respect to body mass, plumage ornamentation, reproductive parameters and habitat quality. The Z* allele was found in 14% of 251 adult birds from nature reserves, but was absent from 33 birds breeding in intensively managed agricultural lands. Males and females with the Z* allele had less extensive breeding plumage, and females had a higher body mass, bred earlier and had larger eggs. There were no significant differences in annual survival between birds with and without the Z* allele. DNA isolated from museum skins demonstrated that this polymorphism was present at low frequency in 1929. We speculate that strong asymmetrical overdominance may explain the low frequency of the Z* allele and that genetic linkage to causal genes might be an explanation for the phenotypic correlations. Our findings suggest a degree of cryptic genetic population structuring in the Dutch Godwit population.

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“…The development of molecular techniques has now facilitated the use of DNA in this process. Genetic data have successfully been used to detect sexing errors in museum bird skins (Lee & Griffiths 2003;Bantock et al 2008) and to verify the identity of species (e.g. Hennache et al 2003;Olson et al 2005) or even eggs .…”

Section: Discussion (A) Erroneous Origins Of Historic Megadyptes Specmentioning

confidence: 99%

Lost in translation or deliberate falsification? Genetic analyses reveal erroneous museum data for historic penguin specimens

et al. 2009

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Historic museum specimens are increasingly used to answer a wide variety of questions in scientific research. Nevertheless, the scientific value of these specimens depends on the authenticity of the data associated with them. Here we use individual-based genetic analyses to demonstrate erroneous locality information for archive specimens from the late nineteenth century. Specifically, using 10 microsatellite markers, we analysed 350 contemporary and 43 historic yellow-eyed penguin (Megadyptes antipodes) specimens from New Zealand's South Island and sub-Antarctic regions. Factorial correspondence analysis and an assignment test strongly suggest that eight of the historic specimens purportedly of sub-Antarctic origin were in fact collected from the South Island. Interestingly, all eight specimens were obtained by the same collector, and all are currently held in the same museum collection. Further inspection of the specimen labels and evaluation of sub-Antarctic voyages did not reveal whether the erroneous data are caused by incorrect labelling or whether deliberate falsification was at play. This study highlights a promising extension to the well-known applications of assignment tests in molecular ecology, which can complement methods that are currently being applied for error detection in specimen data. Our results also serve as a warning to all who use archive specimens to invest time in the verification of collection information.

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Sexing errors among museum skins of a sexually monomorphic bird, the Moorhen Gallinula chloropus

Cited by 12 publications

References 28 publications

Improving non-invasive genotyping in capercaillie (Tetrao urogallus): redesigning sexing and microsatellite primers to increase efficiency on faeces samples

Improving non-invasive genotyping in capercaillie (Tetrao urogallus): redesigning sexing and microsatellite primers to increase efficiency on faeces samples

Linking intronic polymorphism on the CHD1‐Z gene with fitness correlates in Black‐tailed Godwits Limosa l. limosa

Lost in translation or deliberate falsification? Genetic analyses reveal erroneous museum data for historic penguin specimens

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