Sexing chick embryos: A rapid and simple protocol

Clinton, Michael; Haines, L.; Belloir, B.; McBride, Derek

doi:10.1080/713655025

Cited by 123 publications

(77 citation statements)

References 23 publications

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“…The detailed description of the molecular methods applied in the present study is given in Mucha et al (2014b). The molecular identification of the sex of the individuals was determined using the method given by Clinton et al (2001).…”

Section: Molecular Analysismentioning

confidence: 99%

Successful Identification of Duck Genome Region Determining Desirable Uniformity of Meat Performance Traits

Dobek

Gornowicz

Moliński

et al. 2017

Rev. Bras. Cienc. Avic.

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The objective of this study was to identify genome regions determining duck meat performance traits with possible small variation. In total, 368 crossbred ducks of F2 generation obtained from two parental lines: Pekin-type ducks of Polish origin (A55) and Pekin-type ducks of French origin (GL-30) were recorded. The following seven traits were analyzed: body weight, breast muscle weight, leg muscle weight, water holding capacity in the breast and leg muscles, and color lightness L* of the breast and leg muscles. All birds (including parental and F1 generations) were genotyped (29 microsatellite markers). Means and coefficients of variation (CV) were calculated for 28 full-sibs (four sires by six dams and one sire by four dams). Number of progeny per fullsib group ranged from 7 to 17. The multivariate cluster analysis using grouping by k-means algorithm was used on transformed data. The multivariate cluster analysis gave two clusters: first group with 10 fullsibs and second one with 18 families. Differences among half-sibs in the CV of the recorded traits were determined. It should be noted that one out of five sire groups showed statistically significant differences from the other ones. Moreover, the CVs in this group were smaller. The analysis of microsatellite markers indicated three alleles from three loci were present only in the "superior" sire group. The obtained results indicate a promising opportunity of effective selection for improving carcass technological quality using molecular markers.

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Section: Molecular Analysismentioning

confidence: 99%

Successful Identification of Duck Genome Region Determining Desirable Uniformity of Meat Performance Traits

Dobek

Gornowicz

Moliński

et al. 2017

Rev. Bras. Cienc. Avic.

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“…For genetic sexing of embryos, a small piece of limb tissue was digested in PCR compatible Proteinase K buffer (McClive and Sinclair, 2001) and the genomic DNA used for rapid PCR sexing (Clinton et al, 2001). By this method, only females show a W-linked (female-specific) Xho1 band.…”

Section: Pcr Sexingmentioning

confidence: 99%

Genetic evidence against a role for W-linked histidine triad nucleotide binding protein (HINTW) in avian sex determination

Smith¹,

Roeszler²,

Sinclair³

2009

Int. J. Dev. Biol.

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Birds have a ZZ/ZW sex chromosome system, but the mechanism of sex determination remains unknown. The heterogametic sex is female (ZW) and one hypothesis holds that the W chromosome carries a dominant-acting ovary-determining gene. The strongest candidate ovary-determinant on the W chromosome is HINTW, which encodes an aberrant nucleotide hydrolase enzyme. HINTW is conserved amongst all carinate (flying) birds and it is strongly expressed in the gonads and other tissues of female chicken embryos. This and other lines of circumstantial evidence support the proposal that HINTW is the female-determining gene in birds. However, in vivo gain-of-function or loss-of-function studies have not hitherto been reported to test this hypothesis. We tested the potential role of HINTW by mis-expressing it in genetically male (ZZ) embryos, using the RCASBP avian retroviral vector. Strong, widespread expression was delivered throughout the embryo, including the urogenital system, as assessed by whole mount in situ hybridisation. This expression pattern mimicked that seen in normal ZW females, in which HINTW is widely expressed. Strong mis-expression was observed throughout the gonads of genetic male (ZZ) embryos. However, despite strong HINTW expression, ZZ gonads developed normally as bilateral testes. In tissue sections of ZZ urogenital systems transgenic for HINTW, normal testicular histology was observed. Female (ZW) gonads over-expressing HINTW also developed normally, with normal ovarian structure and left/right asymmetry. These results provide genetic evidence against a dominant role for HINTW in avian sex determination.

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“…3). Clinton et al (2001) have also demonstrated the detection of gender of chicken embryos by multiplex PCR using DNA extracted from amniotic fluid cells. The results of sex diagnosis of embryos were same whether DNA extracted from soft tissues or amniotic fluid cells were used for multiplex PCR.…”

Section: Resultsmentioning

confidence: 99%