Sex with two SOX on: SRY and SOX9 in testis development

Clarkson, Michael; Harley, Vincent R.

doi:10.1016/s1043-2760(01)00541-0

Cited by 74 publications

(47 citation statements)

References 58 publications

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“…In addition, Dlg acts critically in the incorporation of the male specific Sox100B expressing mesodermal cells in the posterior male gonads, since this group of male-specific cells is missing in newly formed dlg male gonads. The Sox100B transcription factor expressed in these cells is the homologue of the mammalian Sox9 protein, a critical player in human male sex determination and testis differentiation (Clarkson and Harley, 2002). Further examination of wild type and mutant larval gonads showed that Sox100B expression in the posterior mesodermal cells should be limited to the time when the testis acquires its anterior-posterior axis and initiates germ cell proliferation and differentiation (DeFalco et al, 2003, Nanda et al, 2009.…”

Section: Discussionmentioning

confidence: 99%

“…This cluster of male-specific mesodermal cells can be detected in both male and female stage 14 embryos as a group of cells located near the posterior end of the forming gonads (Hui Yong Loh and Russell, 2000), which becomes integrated only at the posterior part of the male and not of the female gonads (Casper and Van Doren, 2006). These cells express Sox100B (DeFalco et al, 2003, Nanda et al, 2009, the homologue of the Sox9 protein in mice that plays a critical role in sex determination and testis development (Clarkson andHarley, 2002, Cossais et al, 2010). In order to address the role of dlg in the male-specific mesodermal cells, the fate of the Sox100B cells in dlg male gonads was analyzed.…”

Section: Mesodermal Male-specific Sox100b-cells Fail To Associate Witmentioning

confidence: 99%

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The internal structure of embryonic gonads and testis development in Drosophila melanogaster requires scrib, lgl and dlg activity in the soma

Papagiannouli¹

2013

Int. J. Dev. Biol.

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Interest in the mechanism leading to the formation of the germline and its differentiation during Drosophila development, initiated even as soon as the first ever cloned tumour suppressor gene in Drosophila, the lethal (2) giant larvae (lgl), had been identified. Further work has shown that the lgl, as well as discs large-1 (dlg) and scribble (scrib) tumor suppressor genes code for scaffolding proteins associated with either the cytoskeletal matrix or the septate junctions that act in common pathways in various tissues. This study analysed the role of Dlg, Scrib and Lgl in the embryonic gonads and testis of Drosophila melanogaster. Loss of scrib, dlg and lgl had no effect on gonad formation, but Dlg and Scrib in the gonadal mesoderm acted critically in the somatic wrapping of the pole cells and the internal structure of the Drosophila embryonic gonads. Dlg also affected the incorporation of the male-specific Sox100B positive mesodermal cells into the male embryonic gonads, yet Sox100B expression in dlg testis remained unaffected. Analysis at later stages revealed that scrib and lgl expression in the somatic lineage of the Drosophila testis, similar to what was previously shown for dlg, was indispensable for testis development and homeostasis, as depletion of these genes resulted in extensive testes defects. The data presented here emphasize the somatic requirement of Scrib, Dlg and Lgl in embryonic gonads, as well as in the Drosophila testis that underlines the importance of the somatic lineage in the establishment and maintenance of testis formation throughout successive developmental stages.

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Section: Discussionmentioning

confidence: 99%

Section: Mesodermal Male-specific Sox100b-cells Fail To Associate Witmentioning

confidence: 99%

The internal structure of embryonic gonads and testis development in Drosophila melanogaster requires scrib, lgl and dlg activity in the soma

Papagiannouli¹

2013

Int. J. Dev. Biol.

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“…Regulation at the transcriptional level is believed to be the essential mechanism governing the mammalian sex determination cascade (Swain and Lovell-Badge, 1999;Clarkson and Harley, 2002). We propose that an important new wrinckle needs to be added to this view: regulation at the level of splicing.…”

Section: Discussionmentioning

confidence: 98%

“…Despite the lack of direct evidence that human SRY functions as a transcription factor, transcriptional regulation is commonly evoked when people speculate about its mechanism of action (reviewed in Clarkson and Harley, 2002). Recent studies, however, have shown that the SRY and other SOX factors play a role in pre-mRNA splicing in mammalian cells.…”

Section: Splicing Regulators In Mammalian Sex Determinationmentioning

confidence: 99%

Sexy splicing: regulatory interplays governing sex determination fromDrosophilato mammals

Lalli

Ohe

Latorre

et al. 2003

Journal of Cell Science

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A remarkable array of strategies is used to produce sexual differentiation in different species. Complex gene hierarchies govern sex determination pathways, as exemplified by the classic D. melanogaster paradigm,where an interplay of transcriptional, splicing and translational mechanisms operate. Molecular studies support the hypothesis that genetic sex determination pathways evolved in reverse order, from downstream to upstream genes, in the cascade. The recent identification of a role for the key regulatory factors SRY and WT1(+KTS) in pre-mRNA splicing indicates that important steps in the mammalian sex determination process are likely to operate at the post-transcriptional level.

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“…By E12.5, SOX9 is expressed in Sertoli cells, where it persists throughout life, whereas it is not seen at all in the ovary (Kent et al 1996). The key factor regulating AMH expression in mammals is SOX9 (Arango et al 1999;Vidal et al 2001;Clarkson and Harley 2002). SOX proteins bind a specific DNA sequence using a high-mobility group (HMG) domain that mediates interactions with other transcription factor proteins and contains signals for nuclear import.…”

Section: Sex the Mammalian Waymentioning

confidence: 99%