Sex-switching of the<i>Drosophila</i>brain by two antagonistic chromatin factors

Ito, Hiroki; Sato, Kosei; Yamamoto, Daisuke

doi:10.4161/fly.24018

Cited by 16 publications

(12 citation statements)

References 32 publications

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“…In addition, the ecdysone receptor was shown to act in fru neurons to mediate male courtship behavior [ 40 ]. More recently, it was shown that females depleted in ecdysone display male-like courtship behaviors [ 41 ], and it was proposed that distinct ecdysone peaks might regulate the formation of distinct Fru M -containing chromatin regulatory complexes [ 42 ]. Although our developmental time course did not detect a dramatic shift in Fru M DNA-binding specificity as a result of ecdysone pulses, there are more subtle dynamic shifts in binding throughout development that might result from these pulses.…”

Section: Discussionmentioning

confidence: 99%

Male-Specific Fruitless Isoforms Target Neurodevelopmental Genes to Specify a Sexually Dimorphic Nervous System

et al. 2014

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SummaryBackgroundIn Drosophila, male courtship behavior is regulated in large part by the gene fruitless (fru). fru encodes a set of putative transcription factors that promote male sexual behavior by controlling the development of sexually dimorphic neuronal circuitry. Little is known about how Fru proteins function at the level of transcriptional regulation or the role that isoform diversity plays in the formation of a male-specific nervous system.ResultsTo characterize the roles of sex-specific Fru isoforms in specifying male behavior, we generated novel isoform-specific mutants and used a genomic approach to identify direct Fru isoform targets during development. We demonstrate that all Fru isoforms directly target genes involved in the development of the nervous system, with individual isoforms exhibiting unique binding specificities. We observe that fru behavioral phenotypes are specified by either a single isoform or a combination of isoforms. Finally, we illustrate the utility of these data for the identification of novel sexually dimorphic genomic enhancers and novel downstream regulators of male sexual behavior.ConclusionsThese findings suggest that Fru isoform diversity facilitates both redundancy and specificity in gene expression, and that the regulation of neuronal developmental genes may be the most ancient and conserved role of fru in the specification of a male-specific nervous system.

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Section: Discussionmentioning

confidence: 99%

Male-Specific Fruitless Isoforms Target Neurodevelopmental Genes to Specify a Sexually Dimorphic Nervous System

et al. 2014

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“…Failure to execute these steps during development can result in birth defects and infertility, while defects in these processes later in life have been linked to numerous diseases, including cancer and neurodegeneration. The Broad‐complex, Tramtrack and Bric‐à‐brac/poxvirus and zinc finger (BTB/POZ) family of proteins has been widely studied due to its vital role in multiple developmental and disease contexts, including lymphocyte development, axon guidance, gonad morphogenesis, and leukemia (Arama et al, ; Bayarmagnai et al, ; Fan et al, ; Gates et al, ; Ito et al, ; Ko et al, ; Lim, ; Sadler et al, ; Siggs et al, ; Weyers et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…been widely studied due to its vital role in multiple developmental and disease contexts, including lymphocyte development, axon guidance, gonad morphogenesis, and leukemia (Arama et al, 2007;Bayarmagnai et al, 2012;Fan et al, 2015;Gates et al, 2011;Ito et al, 2013;Ko et al, 2006;Lim, 2014;Sadler et al, 2015;Weyers et al, 2011).…”

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confidence: 99%

Broad‐complex, tramtrack, and bric‐à‐brac (BTB) proteins: Critical regulators of development

Chaharbakhshi

Jemc

2016

Genesis

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Broad-complex, Tramtrack, and Bric-à-brac/poxvirus and zinc finger (BTB/POZ) family proteins are a diverse family of proteins that are characterized by the presence of a common protein-protein interaction domain, known as the BTB domain. BTB proteins have been identified in poxviruses and many eukaryotes, and have diverse functions, ranging from transcriptional regulation and chromatin remodeling to protein degradation and cytoskeletal regulation. Specificity of function is determined in part by additional domains present in BTB family proteins, as well as by interaction partners. Studies of BTB proteins in Drosophila and mammalian systems have revealed the importance of these genes in multiple developmental contexts, as well as in cancer and neurological and musculoskeletal diseases. In this review, we discuss the functions of BTB/POZ proteins during development with an emphasis on BTB-zinc finger (BTB-ZF) proteins, which play critical roles in transcriptional regulation and chromatin remodeling.

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“…In particular, adult animals exhibit sexual dimorphisms in morphology, physiology and reproductive behavior, many of which develop, in holometabolous insects, around the pupal stage, when the ecdysone titer changes dynamically ( De Loof, 2008 ; Truman, 2005 ). It is therefore likely that neural circuitries for sexually dimorphic behaviors displayed by adults are laid out during this developmental stage, under the control of ecdysone signaling ( Ito et al, 2013 ). There is evidence that the ecdysone pathway directly contributes to neural remodeling via dendrite pruning ( Awasaki et al, 2006 ; Williams and Truman, 2005 ), and to cell death during metamorphosis by cooperating with epigenetic factors including CREB-binding protein CBP, a histone acetyltransferase (HAT; Kirilly et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

“…There is evidence that the ecdysone pathway directly contributes to neural remodeling via dendrite pruning ( Awasaki et al, 2006 ; Williams and Truman, 2005 ), and to cell death during metamorphosis by cooperating with epigenetic factors including CREB-binding protein CBP, a histone acetyltransferase (HAT; Kirilly et al, 2011 ). This invites speculation that crosstalk between ecdysone signaling and the sex-determination pathway might provide a means for the organism to create sex differences in an otherwise unisexual neural circuitry ( Ito et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Ecdysone signaling regulates specification of neurons with a male-specific neurite inDrosophila

2018

Self Cite

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Some mAL neurons in the male brain form the ipsilateral neurite (ILN[+]) in a manner dependent on FruBM, a male-specific transcription factor. FruBM represses robo1 transcription, allowing the ILN to form. We found that the proportion of ILN[+]-mALs in all observed single cell clones dropped from ∼90% to ∼30% by changing the heat-shock timing for clone induction from 4-5 days after egg laying (AEL) to 6-7 days AEL, suggesting that the ILN[+]-mALs are produced predominantly by young neuroblasts. Upon EcR-A knockdown, ILN[+]-mALs were produced at a high rate (∼60%), even when heat shocked at 6-7 days AEL, yet EcR-B1 knockdown reduced the proportion of ILN[+]-mALs to ∼30%. Immunoprecipitation assays in S2 cells demonstrated that EcR-A and EcR-B1 form a complex with FruBM. robo1 reporter transcription was repressed by FruBM and ecdysone counteracted FruBM. We suggest that ecdysone signaling modulates the FruBM action to produce an appropriate number of male-type neurons.

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Sex-switching of theDrosophilabrain by two antagonistic chromatin factors

Cited by 16 publications

References 32 publications

Male-Specific Fruitless Isoforms Target Neurodevelopmental Genes to Specify a Sexually Dimorphic Nervous System

Male-Specific Fruitless Isoforms Target Neurodevelopmental Genes to Specify a Sexually Dimorphic Nervous System

Broad‐complex, tramtrack, and bric‐à‐brac (BTB) proteins: Critical regulators of development

Ecdysone signaling regulates specification of neurons with a male-specific neurite inDrosophila

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