Sex steroids and leptin regulate 11β-hydroxysteroid dehydrogenase I and P450 aromatase expressions in human preadipocytes: Sex specificities

Dieudonné, Marie‐Noëlle; Sammari, Anes; Santos, Esther Dos; Leneveu, Marie-Christine; Giudicelli, Yves; Pecquery, René

doi:10.1016/j.jsbmb.2006.01.007

Cited by 75 publications

(78 citation statements)

References 57 publications

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“…Adipose tissue is the major tissue site of conversion of androstenedione to estrone, and estradiol has been reported to be elevated in obese humans and non-human primates (reviewed in Siiteri 1987, Dieudonne et al 2006. Maternal body weight was higher overall in the VHF mice in this study ( Fig.…”

Section: Discussionmentioning

confidence: 60%

“…Dietary fat can influence the expression of enzymes that metabolize sex steroid hormones (Zhou et al 2005, Dieudonne et al 2006. Adipose tissue is an important site of steroid hormone biosynthesis (Siiteri 1987, Belanger et al 2002, Simpson 2003, Dieudonne et al 2006.…”

Section: Introductionmentioning

confidence: 99%

“…Adipose tissue is an important site of steroid hormone biosynthesis (Siiteri 1987, Belanger et al 2002, Simpson 2003, Dieudonne et al 2006. Moreover, ovarianderived D4 androstenedione and testosterone can be aromatized in adipose tissue to estrone and estradiol respectively (Lambrinoudaki et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Maternal diet composition alters serum steroid and free fatty acid concentrations and vaginal pH in mice

Whyte¹,

Alexenko²,

Davis³

et al. 2007

Journal of Endocrinology

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We examined the effects of three maternal diets (very high fat (VHF), low fat (LF), and control (Purina 5015)) on serum steroids, free fatty acids (FFA), and vaginal pH in National Institutes of Health Swiss mice. Females were fed (VHF, nZ33; LF, nZ33; 5015, nZ48) from 4 to 16 weeks of age. Following breeding, female serum was collected at 0 . 5 (pre-implantation, early diestrus) or 8 . 5 (post-implantation, mid-diestrus) days post-coitus (dpc). The serum concentrations of 17b-estradiol, testosterone, progesterone, and FFA were analyzed at both collection points, and vaginal pH at 0 . 5 dpc. Striking differences in steroids and FFA were observed at 0 . 5 dpc among the groups. Estradiol was higher in the VHF (14 . 1G3 . 0 pg/ml), compared with LF mice (5 . 2G2 . 3 pg/ml; P%0 . 05). In contrast, 0 . 5 dpc testosterone was lower in the VHF (10 . 5G3 . 0 pg/ml) versus the LF group (32 . 7G8 . 4 pg/ml; P%0 . 05). At 8 . 5 dpc, progesterone was higher in the VHF (89 . 6G6 . 7 ng/ml) versus the 5015 group (60 . 1G4 . 9 ng/ml; P%0 . 05). VHF mice had higher FFA concentrations at 0 . 5 dpc (1 . 0G0 . 2 mmol/l) than LF and control mice (0 . 5G0 . 1 and 0 . 6G0 . 1 mmol/l respectively; P%0 . 05). At 8 . 5 dpc, VHF females had higher serum FFA (0 . 8G0 . 1 mmol/l) than LF and control females (0 . 4G0 . 1 and 0 . 6G0 . 1 mmol/l; P%0 . 05). Mean vaginal pH of VHF females (6 . 41G0 . 09) was lower than 5015 females (6 . 76G 0 . 10; P%0 . 05). These diet-induced alterations in serum steroid and FFA concentrations might affect several reproductive processes, including preferential fertilization by one class of sperm over the other and sex bias in pre-and post-implantational embryonic development.

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Section: Discussionmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

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Maternal diet composition alters serum steroid and free fatty acid concentrations and vaginal pH in mice

Whyte¹,

Alexenko²,

Davis³

et al. 2007

Journal of Endocrinology

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“…Hence, the primary objective of this study was to evaluate whether changes in the expression of PGT modulate CYP19 transcription. Preadipocytes contain much higher levels of aromatase than mature adipocytes and were used as a model system (25). Here, we show that PGT regulates adipogenesis and thereby the PGE 2 !…”

Section: Introductionmentioning

confidence: 80%

The Prostaglandin Transporter Regulates Adipogenesis and Aromatase Transcription

Subbaramaiah

Hudis

Dannenberg

2011

Cancer Prevention Research

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Cytochrome P450 aromatase, encoded by the CYP19 gene, catalyzes estrogen synthesis. In obese postmenopausal women, increased estrogen synthesis in adipose tissue has been linked to hormonedependent breast carcinogenesis. Hence, it is important to elucidate the mechanisms that regulate CYP19 gene expression. Prostaglandin E 2 (PGE 2 ) stimulates the cyclic AMP (cAMP) ! protein kinase A (PKA) ! cAMP responsive element binding protein (CREB) pathway leading to increased CYP19 transcription. The prostaglandin transporter (PGT) removes PGE 2 from the extracellular milieu and delivers it to the cytosol, where it is inactivated. The main objective of this study was to determine whether PGT regulates CYP19 transcription. Silencing of PGT in preadipocytes increased PGE 2 levels in the extracellular medium, thereby stimulating the cAMP ! PKA pathway resulting in enhanced interaction between pCREB, p300, and the CYP19 I.3/II promoter. A reciprocal decrease in the interaction between the CYP19 I.3/II promoter and BRCA1, a repressor of CYP19 transcription, was observed. Overexpressing PGT reduced extracellular PGE 2 levels, suppressed the cAMP ! PKA pathway, enhanced the interaction between BRCA1 and p300, and inhibited aromatase expression. We also compared the PGT ! aromatase axis in preadipocytes versus adipocytes. Aromatase levels were markedly increased in preadipocytes versus adipocytes. This increase in aromatase was explained, at least in part, by reduced PGT levels leading to enhanced PGE 2 ! cAMP ! PKA signaling. In addition to regulating aromatase expression, PGT-mediated changes in extracellular PGE 2 levels were a determinant of adipocyte differentiation. Collectively, these results suggest that PGT modulates adipogenesis and thereby PGE 2 -mediated activation of the cAMP ! PKA ! CREB pathway leading to altered CYP19 transcription and aromatase activity.

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“…Leptin receptor is homologous to gp130 and can activate JAK2, mainly STAT3, but also STAT5, STAT6, STAT1 proteins [74][75][76][77] . SOCS3 is a negative regulator of leptin JAK-STAT signaling [18,78,79] .…”

Section: Leptin Jak-stat Signalingmentioning

confidence: 99%

JAK-STAT pathway in carcinogenesis: Is it relevant to cholangiocarcinoma progression?

Smirnova¹

2007

WJG

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The features of JAK-STAT signaling in liver cells are discussed in the current review. The role of this signaling cascade in carcinogenesis is accentuated. The possible involvement of this pathway and alteration of its elements are compared for normal cholangiocytes, cholangiocarcinoma predisposition and development. Prolactin and interleukin-6 are described in detail as the best studied examples. In addition, the non-classical nuclear translocation of cytokine receptors is discussed in terms of its possible implication to cholangiocarcinoma development.

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Sex steroids and leptin regulate 11β-hydroxysteroid dehydrogenase I and P450 aromatase expressions in human preadipocytes: Sex specificities

Cited by 75 publications

References 57 publications

Maternal diet composition alters serum steroid and free fatty acid concentrations and vaginal pH in mice

Maternal diet composition alters serum steroid and free fatty acid concentrations and vaginal pH in mice

The Prostaglandin Transporter Regulates Adipogenesis and Aromatase Transcription

JAK-STAT pathway in carcinogenesis: Is it relevant to cholangiocarcinoma progression?

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