Sex related differences in the adhesion, migration, and growth of rat aortic smooth muscle cells in culture

Bačáková, Lucie; Lisá,; Pellicciari, C.; Mares,; Mg, Bottone; Kocourek, František

doi:10.1007/s11626-997-0055-9

Cited by 21 publications

(9 citation statements)

References 18 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, poor cell attachment on P6NF also confirmed the need for specific interactions between the catechol moieties and the cells because C6NF and P6NF showed similar surface wettabilities. Researchers have shown that non‐receptor/protein‐mediated cell adhesions to a substrate are mediated via weak chemical bonding, such as hydrogen bonding and electrostatic and ionic interactions between various molecules on a cell membrane and functional chemical groups on a surface of the substrate . However, P6NF showed the lowest cell attachment relative to blank NF; 31.13% of cells attached to the surface after 2 h. This can be explained that the liberated catechol moieties cause lower cell viability, as shown in Figure ; thus, the cell attachment efficiencies decreased accordingly.…”

Section: Resultsmentioning

confidence: 97%

Decoration of Electrospun Nanofibers with Monomeric Catechols to Facilitate Cell Adhesion

Choi

Messersmith

Yoo

2013

Macromolecular Bioscience

View full text Add to dashboard Cite

Monomeric catechols are displayed on the surface of polymeric nanofibers by robust catechol-thiol interactions to enhance cell adhesion and migration. Dihydroxyphenyl propionic acid is chemically conjugated to primary amine groups of poly(ϵ-caprolactone)-poly(ethylene glycol)-amine (PCL-PEG) nanofibers to display catechol moieties on the surface. At basic pH, catecholized nanofibers incorporate thiol groups at a five-fold higher rate than at acidic pH, while catechol-coated surfaces do not show any pH-dependent binding. Live/dead cell staining indicates that the catecholized nanofibers do not exert any cytotoxic effects. Also, NIH 3T3 cells cultured on the catecholized nanofibers show increased attachment and migration that is proportional to the amount of the immobilized catechol moieties on the surface. These results clearly indicate that 6 nmol of monomeric catechols on the surface of nanofiber can promote cell adhesion and migration by thiol-catehol interactions.

show abstract

Section: Resultsmentioning

confidence: 97%

Decoration of Electrospun Nanofibers with Monomeric Catechols to Facilitate Cell Adhesion

Choi

Messersmith

Yoo

2013

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…The materials were fixed to the bottom of the culture wells by plastic rings in order to prevent them floating in the cell culture media, and were seeded with vascular smooth muscle cells (VSMC), derived from the rat aorta by an explantation method [40]. The cells were used in passage 3.…”

Section: Methodsmentioning

confidence: 99%

Adhesion and Growth of Vascular Smooth Muscle Cells on Nanostructured and Biofunctionalized Polyethylene

et al. 2013

View full text Add to dashboard Cite

Cell colonization of synthetic polymers can be regulated by physical and chemical modifications of the polymer surface. High-density and low-density polyethylene (HDPE and LDPE) were therefore activated with Ar+ plasma and grafted with fibronectin (Fn) or bovine serum albumin (BSA). The water drop contact angle usually decreased on the plasma-treated samples, due to the formation of oxidized groups, and this decrease was inversely related to the plasma exposure time (50–300 s). The presence of nitrogen and sulfur on the polymer surface, revealed by X-ray photoelectron spectroscopy (XPS), and also by immunofluorescence staining, showed that Fn and BSA were bound to this surface, particularly to HDPE. Plasma modification and grafting with Fn and BSA increased the nanoscale surface roughness of the polymer. This was mainly manifested on HDPE. Plasma treatment and grafting with Fn or BSA improved the adhesion and growth of vascular smooth muscle cells in a serum-supplemented medium. The final cell population densities on day 6 after seeding were on an average higher on LDPE than on HDPE. In a serum-free medium, BSA grafted to the polymer surface hampered cell adhesion. Thus, the cell behavior on polyethylene can be modulated by its type, intensity of plasma modification, grafting with biomolecules, and composition of the culture medium.

show abstract

“…In our experiments, the cell viability was very high, reaching 95-99%, and was similar for all tested samples. The percentage of dead cells is in the range of physiological cell loss observed in cell populations [36]. Only viable cells were used for the evaluation of the cell adhesion and growth on the tested samples.…”

Section: Endothelial Cell Culturementioning

confidence: 99%

Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers

Filova¹

2014

J Tissue Sci Eng

View full text Add to dashboard Cite

show abstract

Sex related differences in the adhesion, migration, and growth of rat aortic smooth muscle cells in culture

Cited by 21 publications

References 18 publications

Decoration of Electrospun Nanofibers with Monomeric Catechols to Facilitate Cell Adhesion

Decoration of Electrospun Nanofibers with Monomeric Catechols to Facilitate Cell Adhesion

Adhesion and Growth of Vascular Smooth Muscle Cells on Nanostructured and Biofunctionalized Polyethylene

Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers

Contact Info

Product

Resources

About