Sex effect on presenilins expression in post-natal rat brain

Canterini, Sonia; Carletti, Valentina; Rosa, Paolo; Crestini, Alessio; Fiorenza, Maria Teresa; Confaloni, Annamaria

doi:10.4236/abb.2013.412145

Cited by 3 publications

(2 citation statements)

References 33 publications

(37 reference statements)

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“…Coronal sections (thickness, 8 μm) obtained at −2,00 mm from bregma were processed as previously described [51,52,55]. Briefly, antigen retrieval was achieved by pre-treating sections with 10 mM sodium citrate pH 6.0 in a microwave oven for 5 min; then, sections were incubated overnight at 4°C with a mouse monoclonal primary antibody against PSEN1 (#MAB5232, Millipore; 1:500 final dilution in PBS), washed 5 times with PBS, and stained using the mouse Vectastain Elite ABC Kit (Vector Laboratories Inc., cat.…”

Section: Immunohistochemistrymentioning

confidence: 99%

CpG and non-CpG Presenilin1 methylation pattern in course of neurodevelopment and neurodegeneration is associated with gene expression in human and murine brain

et al. 2020

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The Presenilin1 (PSEN1) gene encodes the catalytic peptide of the γ-secretase complex, a key enzyme that cleaves the amyloid-β protein precursor (AβPP), to generate the amyloid-β (Aβ) peptides, involved in Alzheimer's Disease (AD). Other substrates of the γ-secretase, such as Ecadherin and Notch1, are involved in neurodevelopment and haematopoiesis. Gene-specific DNA methylation influences PSEN1 expression in AD animal models. Here we evaluated canonical and non-canonical cytosine methylation patterns of the PSEN1 5ʹ-flanking during brain development and AD progression, in DNA extracted from the frontal cortex of AD transgenic mice (TgCRND8) and post-mortem human brain. Mapping CpG and non-CpG methylation revealed different methylation profiles in mice and humans. PSEN1 expression only correlated with DNA methylation in adult female mice. However, in post-mortem human brain, lower methylation, both at CpG and non-CpG sites, correlated closely with higher PSEN1 expression during brain development and in disease progression. PSEN1 methylation in blood DNA was significantly lower in AD patients than in controls. The present study is the first to demonstrate a temporal correlation between dynamic changes in PSEN1 CpG and non-CpG methylation patterns and mRNA expression during neurodevelopment and AD neurodegeneration. These observations were made possible by the use of an improved bisulphite methylation assay employing primers that are not biased towards non-CpG methylation. Our findings deepen the understanding of γ-secretase regulation and support the hypothesis that epigenetic changes can promote the pathophysiology of AD. Moreover, they suggest that PSEN1 DNA methylation in peripheral blood may provide a biomarker for AD.

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Section: Immunohistochemistrymentioning

confidence: 99%

CpG and non-CpG Presenilin1 methylation pattern in course of neurodevelopment and neurodegeneration is associated with gene expression in human and murine brain

et al. 2020

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“…In present study, male mice were used. In earlier report, Piscopo et al (2013) showed altered expression of PS1 and PS2 at postnatal stages of rat cerebral cortex development. The alteration in expression pattern was similar in male and female for PS1 as well as PS2 during development but the extent of alteration was variable.…”

Section: Proteinmentioning

confidence: 72%

Analysis of Presenilin 1 and 2 interacting proteins in mouse cerebral cortex during development

Kumar

Thakur

2014

Intl J of Devlp Neuroscience

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In our previous report, we showed that Presenilin (PS)1 and 2 have differential expression profile from early embryonic stages till adulthood in mouse cerebral cortex, suggesting that both of these proteins are crucial for brain development. Genetic manipulation studies have also shown the involvement of PS1 in brain development, but PS2 remains largely unexplored. In order to understand how PS1 and 2 mediate developmental functions, we have investigated the interaction of PS1 and 2 with proteins of mouse cerebral cortex during development. Co-immunoprecipitation (Co-IP) combined with MALDI-MS/MS analysis revealed 12 interacting partners of PS1 and 11 partners of PS2. The interacting proteins were different for PS1 and 2, and involved in cell division, glycolysis, cell adhesion and protein trafficking. Densitometric analysis of protein bands visualized after SDS-PAGE separation of Co-IP proteins revealed variation in their amount and degree of interaction during different developmental stages of mice. Further, immunoblot based validation of PS1 interacting protein Notch-1 showed maximum interaction at embryonic day (E) 12.5, decline at E18.5, upregulation from postnatal day 0 (P0) to P20 and thereafter reduction at P45 and 20 weeks. In-silico analysis of PS and its interacting proteins indicated conformation based interaction through common type of secondary structures having alpha helical, extended beta strand and random coil, and CK2, PKC phosphorylation and myristoylation motifs. Taken together, our study showed that PS1 and PS2 interact to varying extent with different proteins of mouse cerebral cortex and suggests their interaction based on specific conformation and involvement in diverse functions essential for the brain development.

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Sexually Dimorphic Expression of Reelin in the Brain of a Mouse Model of Alzheimer Disease

et al. 2016

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Recent evidence highlights the protective role of reelin against amyloid β (Aβ)-induced synaptic dysfunction and cognitive impairment in Alzheimer disease (AD). In this study, exploiting TgCRND8 mice that overexpress a mutant form of amyloid β precursor protein (AβPP) and display an early onset of AD neuropathological signs, we addressed the question whether changes of reelin expression eventually precede the appearance of Aβ-plaques in a sex-dependent manner. We show that sex-associated and brain region-specific differences in reelin expression appear long before Aβ-plaque formation. However, in spite of a downregulation of reelin expression compared to males, TgCRND8 females display fewer Aβ-plaques, suggesting that additional factors, other than sex and reelin level, influence amyloidosis in this mouse model.

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Sex effect on presenilins expression in post-natal rat brain

Cited by 3 publications

References 33 publications

CpG and non-CpG Presenilin1 methylation pattern in course of neurodevelopment and neurodegeneration is associated with gene expression in human and murine brain

CpG and non-CpG Presenilin1 methylation pattern in course of neurodevelopment and neurodegeneration is associated with gene expression in human and murine brain

Analysis of Presenilin 1 and 2 interacting proteins in mouse cerebral cortex during development

Sexually Dimorphic Expression of Reelin in the Brain of a Mouse Model of Alzheimer Disease

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