Sex differences in voluntary oral nicotine consumption by adolescent mice: a dose-response experiment

Klein, Laura Cousino; Stine, Michele M.; Vandenbergh, David J.; Whetzel, Courtney A.; Kamens, Helen M.

doi:10.1016/j.pbb.2004.01.005

Cited by 87 publications

(90 citation statements)

References 61 publications

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“…Accordingly, future studies on the biological bases of ethanol and nicotine interactions are necessary and the use of other strains and species may provide valuable data. Though nicotine concentration may seem high in the drinking solution, nicotine absorption from the buccal cavity yields cotinine plasma levels (Klein et al, 2003(Klein et al, , 2004Sparks and Pauly, 1999) comparable to those found in adolescent smokers (Binnie et al, 2004;Caraballo et al, 2004;Wood et al, 2004). As for ethanol, according to previous studies in adolescent rodents, even the moderate doses used in the present study result in blood levels well above the legal driving limit (Silveri and Spear, 2000).…”

Section: Methodological Issuessupporting

confidence: 73%

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Combined Exposure to Nicotine and Ethanol in Adolescent Mice Differentially Affects Anxiety Levels during Exposure, Short-Term, and Long-Term Withdrawal

Abreu‐Villaça

Nunes

Queiroz-Gomes

et al. 2007

Neuropsychopharmacol

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Smoking and consumption of alcoholic beverages are frequently associated during adolescence. This association could be explained by the cumulative behavioral effects of nicotine and ethanol, particularly those related to anxiety levels. However, despite epidemiological findings, there have been few animal studies of the basic neurobiology of the combined exposure in the adolescent brain. In the present work we assessed, through the use of the elevated plus maze, the short-and long-term anxiety effects of nicotine (NIC) and/or ethanol (ETOH) exposure during adolescence (from the 30th to the 45th postnatal day) in four groups of male and female C57BL/6 mice: (1) Concomitant NIC (nicotine free-base solution (50 mg/ml) in 2% saccharin to drink) and ETOH (ethanol solution (25%, 2 g/kg) i.p. injected every other day) exposure; (2) NIC exposure; (3) ETOH exposure; (4) Vehicle. C57BL/6 mice were selected, in spite of the fact that they present slower ethanol metabolism, because they readily consume nicotine in the concentration used in the present study. During exposure (45th postnatal day: PN45), our results indicated that ethanol was anxiolytic in adolescent mice and that nicotine reverted this effect. Short-term drug withdrawal (PN50) elicited sex-dependent effects: exposure to nicotine and/or ethanol was anxiogenic only for females. Although neither nicotine nor ethanol effects persisted up to 1 month postexposure (PN75), the coadministration elicited an anxiogenic response. In spite of the fact that generalizations based on the results from a single strain of mice are prone to shortcomings, our results suggest that the deficient response to the anxiolytic effects of ethanol in adolescents co-exposed to nicotine may drive higher ethanol consumption. Additionally, increased anxiety during long-term smoking and drinking withdrawal may facilitate relapse to drug use.

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Section: Methodological Issuessupporting

confidence: 73%

“…C57BL/6 mice were chosen because prior reports demonstrate that adult and periadolescent mice from this strain consume nicotine in the concentration used in the present study (Klein et al, 2003(Klein et al, , 2004Sparks and Pauly, 1999). All mice were bred and maintained in our laboratory.…”

Section: Animal Treatment and Behavioral Testingmentioning

confidence: 99%

Combined Exposure to Nicotine and Ethanol in Adolescent Mice Differentially Affects Anxiety Levels during Exposure, Short-Term, and Long-Term Withdrawal

Abreu‐Villaça

Nunes

Queiroz-Gomes

et al. 2007

Neuropsychopharmacol

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“…Our results in the two-bottle choice paradigm indicated that males consumed less nicotine as the concentration of nicotine increased, whereas females consumed a consistent amount of nicotine across a range of concentrations. The observation that adult male mice consumed less nicotine than water, compared with female mice, after adjusting for body weight, is consistent with a study in which female adolescent mice consumed more nicotine, compared with males, after adjusting for body weight and as a percentage of total fl uid intake ( Klein et al, 2004 ).…”

Section: Discussionsupporting

confidence: 88%

“…Higher concentrations of nicotine (up to 60 m g/ml) in the C57BL/6:129SvEv hybrid mice caused some lethality in both males and females (data not shown). As a result, we were not able to evaluate preference for nicotine consumption, as has been demonstrated in a number of other mouse strains ( Klein et al, 2004 ;Li, Karadsheh, Jenkins, & Stitzel, 2005 ;Meliska et al, 1995 ;Robinson et al, 1996 ). Indeed, other studies have used considerably higher concentrations of nicotine in two-bottle choice paradigms, up to 200 m g/ ml; however, none of these studies included the 129SvEv strain alone or as part of an F1 hybrid line.…”

Section: Discussionmentioning

confidence: 90%

“…This procedure measures directly the amount of nicotine consumed and enables investigators to distinguish direct nicotine effects from nicotineassociated cues, a prominent feature of the place-conditioning paradigm. Two-bottle choice has been reported to demonstrate both aversion and preference for nicotine in mice depending on nicotine concentration ( Adriani, Macri, Pacifi ci, & Laviola, 2002 ;Agatsuma et al, 2006 ;Klein, Stine, Pfaff, & Vandenbergh, 2003 ;Klein, Stine, Vandenbergh, Whetzel, & Kamens, 2004 ;Lee, Chen, Shih, & Hiroi, 2004 ;Meliska, Bartke, McGlacken, & Jensen, 1995 ;Robinson, Marks, & Collins, 1996 ;Zhu et al, 2005 ). In a twobottle choice paradigm, mice are exposed to two bottles: one containing water and the other nicotine, the locations of which are switched every few days to prevent conditioned place associations from masking drug effects ( Klein, 2001 ;Klein et al, 2004 ;Shaham, Alvares, Nespor, & Grunberg, 1992 ;Todte et al, 2001 ).…”

Section: Introductionmentioning

confidence: 99%

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Sex differences in response to nicotine in C57Bl/6:129SvEv mice

Isiegas

Mague²,

Blendy³

2009

Nicotine &Amp; Tobacco Research

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Results:Females responded more to the conditioned rewarding effects of nicotine compared with males. Males, however, seemed more responsive to the pharmacological aspects of nicotine by reducing nicotine drinking at higher concentrations; females maintained consistent levels of intake over several doses of nicotine. Male and female mice demonstrated similar locomotor and antinociceptive responses to nicotine. Discussion: These data suggest that place-conditioning and two-bottle choice paradigms may be more sensitive measures of sexual dimorphism in the C57BL/6:129SvEv hybrid mouse than are locomotor or nociception assays.

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Differential sensitivity to the effects of nicotine and bupropion in adolescent and adult male OF1 mice during social interaction tests

Gómez

Carrasco

Redolat

2008

Aggressive Behavior

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Few studies have compared the action of both nicotine (NIC) and bupropion (BUP), an antidepressant used to treat NIC dependence, on social and aggressive behavior at different ages. This study aims to determine whether these drugs produce differential effects in adolescent (postnatal day: 36-37) and adult (postnatal day: 65-66) mice that have been housed individually for 2 weeks in order to induce aggressive behavior. Mice received BUP (40, 20, or 10 mg/kg), NIC (1, 0.5, and 0.25 mg/kg as base), or vehicle earlier to a social interaction test. BUP (40 mg/kg) decreased social investigation and increased nonsocial exploration in both adolescent and adult mice. The same effects were also observed in adult mice administered with a lower dose of the same drug (20 mg/kg). In adolescents, NIC (1 mg/kg) decreased social investigation, but this effect did not reach statistical significance in adults. In conclusion, a differential sensitivity to the effects of NIC or BUP emerged in some of the behavioral categories when the two age groups were compared.

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Sex differences in voluntary oral nicotine consumption by adolescent mice: a dose-response experiment

Cited by 87 publications

References 61 publications

Combined Exposure to Nicotine and Ethanol in Adolescent Mice Differentially Affects Anxiety Levels during Exposure, Short-Term, and Long-Term Withdrawal

Combined Exposure to Nicotine and Ethanol in Adolescent Mice Differentially Affects Anxiety Levels during Exposure, Short-Term, and Long-Term Withdrawal

Sex differences in response to nicotine in C57Bl/6:129SvEv mice

Differential sensitivity to the effects of nicotine and bupropion in adolescent and adult male OF1 mice during social interaction tests

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