Sex differences in nitrite/nitrate levels and antioxidant defense in rat brain

Taşkıran, Dilek; Kutay, Fatma Z.; Sözmen, Eser Yıldırım; Pöğün, Şakire

doi:10.1097/00001756-199703030-00013

Cited by 61 publications

(34 citation statements)

References 19 publications

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“…Furthermore, Thorup et al (1999) have shown that picomolar concentrations of CO, in renal resistance arteries from rats, facilitate the release of NO from a large intracellular pool, whereas micromolar concentrations of CO inhibit NOS activity and NO generation. Our results, in accordance with our previous findings, showed that while NO 2 À +NO 3 À levels were higher in the male hippocampus than the female (Taskiran et al, 1997), CO treatment had no effect on total NO 2 À +NO 3 À levels both in the male and female rat brain. Although this finding is not consistent with some of the previous studies mentioned above, it may be explained by the observation of Stamler and Piantadosi (1996).…”

Section: Discussionsupporting

confidence: 93%

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Effect of Carbon Monoxide on Dopamine and Glutamate Uptake and cGMP Levels in Rat Brain

Taşkıran

Kutay

Pöğün

2002

Neuropsychopharmacol

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After the recognition of nitric oxide (NO) as a messenger molecule in the nervous system, carbon monoxide (CO) has received attention with similar properties. The present study aims to elucidate the effects of CO on synaptosomal dopamine ( 3 H-DA) and glutamate ( 3 H-Glu) uptake and on cGMP levels; possible interaction between NO and CO systems was also evaluated. Our results provide evidence for the inhibition of DA and Glu uptake by CO in a time-, dose-, and temperature-dependent manner in rat striatum and hippocampus, respectively; the inhibition observed was sexually dimorphic with more pronounced effects in females. Basal cGMP levels were higher in female rats than males in the striatum and exogenous CO increased striatal cGMP levels only in males; no effect of CO was observed in the hippocampus. In vivo nitric oxide synthase (NOS) inhibition increased DA and Glu uptake; however, CO was still effective in inhibiting uptake following NOS inhibiton. Taken together, these findings suggest a role for CO in trans-synaptic regulation through modulation of DA and Glu transporters and of cGMP levels; the effect on cGMP levels is independent of NOS activity and appears to be sexually dimorphic and region specific.

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Section: Discussionsupporting

confidence: 93%

“…in the presence of NADPH. NO 2 À levels were determined spectrophotometrically by the Griess reaction (Giovannoni et al, 1997;Taskiran et al, 1997). Sodium nitrate solutions were used for standard measurements.…”

Section: Uptake Assaysmentioning

confidence: 99%

Effect of Carbon Monoxide on Dopamine and Glutamate Uptake and cGMP Levels in Rat Brain

Taşkıran

Kutay

Pöğün

2002

Neuropsychopharmacol

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“…This mixture was then centrifuged at 3000Â g for 20 min and the supernatants were used for the assays as described previously. 21,22 Light Microscopy…”

Section: Determining the Tissue Total No(no X ) Levelsmentioning

confidence: 99%

Protective Effect of Melatonin and 1,25-Dihydroxyvitamin D3 on Renal Ischemia–Reperfusion Injury in Rats

et al. 2013

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Ischemia-reperfusion (I/R) injury induces the generation of reactive oxygen species (ROS) which affect many organs. This study was designed to investigate the roles of melatonin and 1,25-dihydroxyvitamin D 3 (VD3) on renal I/R injury. Thirty male Wistar albino rats were divided into five groups: group 1, control; group 2, right nephrectomy (RN) þ I/R in the contralateral kidney; group 3, melatonin þ RN þ I/R; group 4, VD 3 þ RN þ I/R; and group 5, melatonin þ VD 3 þ RN þ I/R. Melatonin (10 mg/kg), VD3 (0.5 μg/kg), and melatonin plus VD3 were injected intraperitoneally for 7 days before renal I/R. After 7 days, right nephrectomy was initially performed and left renal artery was clamped for 45 min. After 45-min reperfusion, the serum and kidney tissue samples were obtained for assays. Melatonin and VD3 had an ameliorative effect on biochemical parameters such as serum creatinine (SCr) and blood urea nitrogen (BUN). Renal tissue malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO) levels, and superoxide dismutase (SOD) activity were determined. Renal I/R decreased the kidney tissue GSH levels and SOD activity and increased the NO levels as compared with control group. However, melatonin and VD3 and melatonin plus VD3 treatment significantly increased the tissue GSH levels and SOD activity and decreased the NO levels compared with those of I/R group. Meanwhile, MDA levels were not different between the control and I/R groups. But, MDA levels decreased in all treated groups compared to I/R and control groups. These data support that melatonin and VD3 have beneficial effects on renal injury.

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“…Nitrite plus nitrate (NOx) which are the last and stabile products of NO were determined in tissue samples spectrophotometrically (21).…”

Section: Nox Assaymentioning

confidence: 99%

The effect of intracerebroventricular injection of beta amyloid peptide (1-42) on caspase-3 activity, lipid peroxidation, nitric oxide and nos expression in young adult and aged rat brain

Çetin

Yazıhan²,

Dinçer³

et al. 2012

Turkish Neurosurgery

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AIm: Intracerebroventricular (icv) administration of beta amyloid peptide (Aβ) in rats can be used to model certain aspects of Alzheimer disease (AD).The purpose of this study was to examine the effects of intracerebroventricular Aβ (1-42) peptide injection on caspase-3 activity and expression of nNOS and iNOS, malondialdehyde (MDA), glutathione (GSH) and NOx in the hippocampus, temporal cortex and parietal cortex. mAterIAl and methOds: Groups were defined as 1) young adult control, 2) Aβ (1-42) injected young adult, 3) aged control and 4) Aβ (1-42) injected aged group. Stereotaxic surgery was performed. Aβ (1-42) peptide (5μg/1μl, in each icv) was administered bilateral intracerebroventricularly as a single injection.results: Caspase-3 activity significantly increased in Aβ (1-42) injected aged rats when compared with young adult rats. Aβ (1-42) significantly increased lipid peroxidation in both young adult and aged rats. There was an increase in nNOS expression in the temporal cortex of Aβ (1-42) injected aged rats. COnClusIOn:The most significant increase was seen in hippocampus in caspase-3 levels of the Aged-Aβ 1-42 group. nNOS expression in the hippocampus of aged rats was increased compared to young adult rats. However, nNOX expression in the hippocampus of Aβ (1-42) injected aged rats decreased significantly.KeywOrds: Beta amyloid, Caspase 3 activity, Lipid peroxidation, Nitric oxide, Nitric oxide synthase, Hippocampus, Aging ÖZ AmAÇ: Literatür bulguları, oksidatif stres ve kaspaz aktivitesinin amiolid beta peptid (Aβ)'in meydana getirdiği Alzheimer Hastalığı(AH) nörotoksisitesinde ve yaşlanmada rolü olduğunu göstermektedir. İntraserebroventrikuler (icv) Aβ peptid uygulamaları sıçanlarda AH'nı taklit etmek için kullanılmaktadır. Bu çalışmanın amacı, icv Aβ (1-42) peptid uygulamasının 3-4 aylık genç erişkin ve 22-24 aylık yaşlı sıçanların hipokampus, temporal korteks ve parietal kortekslerinde kaspaz 3 aktivitesi, nNOS ve iNOS ekspresyonu, MDA,GSH ve NO düzeylerine olan etkilerini araştırmaktır. yÖntem ve GereÇler: Gruplar: 1) Genç erişkin kontrol, 2) icv Aβ (1-42) enjekte edilen genç erişkin, 3) Yaşlı kontrol 4) icv Aβ (1-42) enjekte edilen yaşlı grup. Stereotaksik cerrahi uygulanarak her bir serebral ventrikül içerisine 5µg/1µl Aβ(1-42) peptid tek bir kez enjekte edildi. Aβ (1-42) uygulamasından 72 saat sonra sıçanlar dekapite edilerek beyinleri hızla buz üzerinde çıkarıldı.BulGulAr: Aβ(1-42) enjekte edilen yaşlı sıçanlarda genç erişkinlere göre kaspaz 3 aktivitesi anlamlı olarak yüksek bulundu. Aβ(1-42) enjekte edilen hem genç erişkin hem de yaşlı sıçanların temporal ve parietal kortekslerinde MDA düzeyleri anlamlı düzeyde artış gösterdi. nNOS ekspresyonu yaşlı kontrol grubunda genç erişkin kontrol gruba göre hipokampuste yüksek olarak bulundu. sOnuÇ: Yine hipokampuste Aβ(1-42) uygulamasından sonra yaşlı grupta yaşlı kontrol grubuna göre nNOS ekspresyonunda azalma bulundu. Temporal kortekste ise nNOS ekspresyonu Aβ(1-42) uygulamasından sonra yaşlı grupta artmış bulundu. iNOS ekspresyonu ise hiç...

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Sex differences in nitrite/nitrate levels and antioxidant defense in rat brain

Cited by 61 publications

References 19 publications

Effect of Carbon Monoxide on Dopamine and Glutamate Uptake and cGMP Levels in Rat Brain

Effect of Carbon Monoxide on Dopamine and Glutamate Uptake and cGMP Levels in Rat Brain

Protective Effect of Melatonin and 1,25-Dihydroxyvitamin D3 on Renal Ischemia–Reperfusion Injury in Rats

The effect of intracerebroventricular injection of beta amyloid peptide (1-42) on caspase-3 activity, lipid peroxidation, nitric oxide and nos expression in young adult and aged rat brain

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