Sex differences in mouse heart rate and body temperature and in their regulation by adenosine A<sub>1</sub> receptors

Yang, Jiangning; Tiselius, C.; Daré, Elisabetta; Johansson, Björn; Valen, Guro; Fredholm, Bertil B.

doi:10.1111/j.1365-201x.2007.01690.x

Cited by 62 publications

(76 citation statements)

References 51 publications

(80 reference statements)

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“…Acute administration of caffeine (7.5 and 30 mg/kg) had little effect on HR (results not shown) as reported earlier (51). Injection of enprofylline (A 2B R antagonist, at the doses of 7.5 and 30 mg/kg) increased HR in male WT mouse from 529 Ϯ 23 to 590 Ϯ 20 and 562 Ϯ 20 after the low and high dose, respectively (means Ϯ SE; n ϭ 6), but not in females (data not shown).…”

Section: Resultssupporting

confidence: 72%

“…We used a telemetry system (Data Sciences, St. Paul, MN) consisting of implantable transmitters (TA10ETA-F20), telemetry receivers (DSI PhysioTel Receivers-RPC-1 Model), and eight universal adapters (UA 10 PC) as previously described (51). A computer program (Data quest A.R.T gold acquisition) sampled calibrated values of HR and Temp as well as noncalibrated LA counts.…”

Section: Animalsmentioning

confidence: 99%

“…To prevent the infection of mice from the operation, all the instruments and transmitters were sterilized before used in the surgery. The protocol of surgery was described previously (51). Briefly, under anesthesia induced and maintained by isoflurane, the transmitter (TA10ETA-F20) was implanted into the peritoneal cavity and the leads were sutured in a lead II position.…”

Section: Animalsmentioning

confidence: 99%

“…The parameters we examined were heart rate (HR), body temperature (Temp), locomotor activity (LA), and oxygen consumption (O 2 C) since they could be examined in awake animals and for prolonged periods of time. Acute or single-dose caffeine treatment mainly showed a stimulating effect on HR but clearly biphasic effects on Temp and LA (18,27,36,51). We examined not only acute effects of injecting caffeine but also the effect of adding caffeine to the drinking water for 7 days.…”

mentioning

confidence: 99%

“…We have also determined whether the genotype per se leads to changes, since that would indicate an important role of endogenous adenosine in the physiological regulation. Since a previous study (51) revealed major sex differences, we examined both male and female mice.…”

mentioning

confidence: 99%

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Physiological roles of A₁ and A_2A adenosine receptors in regulating heart rate, body temperature, and locomotion as revealed using knockout mice and caffeine

Yang

Chen²,

Fredholm³

2009

American Journal of Physiology-Heart and Circulatory Physiology

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Yang JN, Chen JF, Fredholm BB. Physiological roles of A1 and A2A adenosine receptors in regulating heart rate, body temperature, and locomotion as revealed using knockout mice and caffeine. Am J Physiol Heart Circ Physiol 296: H1141-H1149, 2009. First published February 13, 2009 doi:10.1152/ajpheart.00754.2008.-Heart rate (HR), body temperature (Temp), locomotor activity (LA), and oxygen consumption (O2C) were studied in awake mice lacking one or both of the adenosine A 1 or A2A receptors (A1R or A2AR, respectively) using telemetry and respirometry, before and after caffeine administration. All parameters were lower during day than night and higher in females than males. When compared with wild-type (WT) littermates, HR was higher in male A 1R knockout (A1RKO) mice but lower in A2ARKO mice and intermediate in A1-A2AR double KO mice. A single dose of an unselective ␤-blocker (timolol; 1 mg/kg) abolished the HR differences between these genotypes. Deletion of A 1Rs had little effect on Temp, whereas deletion of A 2ARs increased it in females and decreased it in males. A 1-A2ARKO mice had lower Temp than WT mice. LA was unaltered in A 1RKO mice and lower in A 2ARKO and A1-A2ARKO mice than in WT mice. Caffeine injection increased LA but only in mice expressing A 2AR. Caffeine ingestion also increased LA in an A 2AR-dependent manner in male mice. Caffeine ingestion significantly increased O 2C in WT mice, but less in the different KO mice. Injection of 30 mg/kg caffeine decreased Temp, especially in KO mice, and hence in a manner unrelated to A 1R or A 2AR blockade. Selective A2B antagonism had little or no effect. Thus A 1R and A2AR influence HR, Temp, LA, and O2C in mice in a sex-dependent manner, indicating effects of endogenous adenosine. The A2AR plays an important role in the modulation of O2C and LA by acute and chronic caffeine administration. There is also evidence for effects of higher doses of caffeine being independent of both A 1R and A2AR. diurnal rhythm; metabolic rate; locomotor activity; adenosine; telemetry CAFFEINE (1,3,7-trimethylxanthine), the most widely consumed stimulant in the world, acts as a competitive antagonist of adenosine receptors (ARs) (18). It is much more potent on three of the known receptors, A 1 , A 2A , and A 2B (A 1 R, A 2A R, and A 2B R, respectively) than on the fourth, the A 3 R (17). Since a competitive antagonist will have effects only when and where receptors are activated by an agonist, it is relevant that endogenous agonist adenosine is more potent on A 1 R and A 2A R than on A 2B R. The potency of adenosine as an agonist in addition depends on the density of the receptors (16). The levels of adenosine are low under physiological condition but are sufficient to partially activate A 1 R, A 2A R, and A 3 Rs where the receptors are abundantly expressed. For these reasons caffeine is generally believed to act on A 1 R and A 2A R to exert most of its effects, at least when given in lower doses (18). However, a recent study showed that A 2B R knockout (A 2B RKO) mice have e...

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Section: Resultssupporting

confidence: 72%

Section: Animalsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Physiological roles of A₁ and A_2A adenosine receptors in regulating heart rate, body temperature, and locomotion as revealed using knockout mice and caffeine

Yang

Chen²,

Fredholm³

2009

American Journal of Physiology-Heart and Circulatory Physiology

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Role of dopamine D₁ receptors in caffeine‐mediated ERK phosphorylation in the rat brain

Acquas

Vinci

Ibba

et al. 2009

Synapse

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The aim of this research was to study the role of dopamine D(1) receptors in caffeine elicited ERK phosphorylation in the prefrontal and other cortical (cingulate and motor) and subcortical (shell and core of the nucleus accumbens) regions. To this end, caffeine (3 and 10 mg/kg) was administered before phosphoERK immunohistochemistry. Caffeine dose-dependently increased the number of phosphoERK-positive neurons in the prefrontal and cingulate cortices but not in the secondary motor cortex and in the nucleus accumbens shell and core. The dopamine D(1) receptor antagonist, SCH 39166 (50 microg/kg), fully prevented phosphoERK activation by caffeine (10 mg/kg) in the superficial and deep layers of the prefrontal cortex but failed to prevent it in the cingulate cortex. Given that phosphoERK can be regarded as a postsynaptic marker of neuronal activation, the present results indicate that psychotropic properties of caffeine may result from the activation of prefrontal, via dopamine D(1) receptors, and cingulate cortices. Failure of caffeine to activate ERK in the nucleus accumbens further supports, indirectly, the observation that caffeine fails to activate dopamine transmission in this structure and is consistent with the tenet that caffeine lacks of true addictive properties.

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Targeting Purinergic Signaling and Cell Therapy in Cardiovascular and Neurodegenerative Diseases

Andrejew

Glaser

Oliveira-Giacomelli

et al. 2019

Advances in Experimental Medicine and Biology

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Sex differences in mouse heart rate and body temperature and in their regulation by adenosine A₁ receptors

Abstract: Thus, the adenosine A(1) receptor is involved in the regulation of heart rate, body temperature and locomotor activity, but the magnitude of the involvement is different in males and females.

Cited by 62 publications

References 51 publications

Physiological roles of A₁ and A_2A adenosine receptors in regulating heart rate, body temperature, and locomotion as revealed using knockout mice and caffeine

Physiological roles of A₁ and A_2A adenosine receptors in regulating heart rate, body temperature, and locomotion as revealed using knockout mice and caffeine

Role of dopamine D₁ receptors in caffeine‐mediated ERK phosphorylation in the rat brain

Targeting Purinergic Signaling and Cell Therapy in Cardiovascular and Neurodegenerative Diseases

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Sex differences in mouse heart rate and body temperature and in their regulation by adenosine A1 receptors

Abstract: Thus, the adenosine A(1) receptor is involved in the regulation of heart rate, body temperature and locomotor activity, but the magnitude of the involvement is different in males and females.

Cited by 62 publications

References 51 publications

Physiological roles of A1 and A2A adenosine receptors in regulating heart rate, body temperature, and locomotion as revealed using knockout mice and caffeine

Physiological roles of A1 and A2A adenosine receptors in regulating heart rate, body temperature, and locomotion as revealed using knockout mice and caffeine

Role of dopamine D1 receptors in caffeine‐mediated ERK phosphorylation in the rat brain

Targeting Purinergic Signaling and Cell Therapy in Cardiovascular and Neurodegenerative Diseases

Contact Info

Product

Resources

About

Sex differences in mouse heart rate and body temperature and in their regulation by adenosine A₁ receptors

Physiological roles of A₁ and A_2A adenosine receptors in regulating heart rate, body temperature, and locomotion as revealed using knockout mice and caffeine

Physiological roles of A₁ and A_2A adenosine receptors in regulating heart rate, body temperature, and locomotion as revealed using knockout mice and caffeine

Role of dopamine D₁ receptors in caffeine‐mediated ERK phosphorylation in the rat brain