Sex Differences in Hepatic Lipids of Toxaphene-Exposed Juvenile Yellowtail Flounder (Pleuronectes ferrugineus Storer)

Scott, K.D.; Ree, Goverdina Elisabeth Fåhræus-Van; Parrish, Christopher C.

doi:10.1006/eesa.2001.2135

Cited by 13 publications

(6 citation statements)

References 20 publications

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“…The same results were obtained in cultured 0 + year class yellowtail flounder (Scott et al 2002). Biochemical studies demonstrated that the amount of lipid stored by cultured sexually immature yellowtail flounder (0 + ) was significantly higher than the amount stored by wild fish, but the differences were not sex dependent (Whalen 1999;Scott et al 2002). In contrast, sex differences in hepatocytes have been reported in sexually mature winter flounder, with males having a lower and less variable lipid content during the reproductive cycle than females, reflecting the process of vitellogenesis in females (March and Reisman 1995).…”

Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 55%

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Structure of and energy reserves in the liver of wild and cultured yellowtail flounder, Limanda ferruginea

Ree

Spurrell

2003

Marine Biology

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Knowledge of the structure and energy reserves, in the liver of commercially important fish species, is important in understanding metabolic processes and in assessing the impact of potential environmental physical and chemical stressors in both wild and cultured stocks. The present study investigated the microscopic morphology and histochemistry (total and neutral lipids, glycogen) of liver tissue of wild (3 + ) and cultured (1 + ) sexually immature female and male yellowtail flounder (Limanda ferruginea Storer), sampled in late April 2001. Hepatosomatic indices [HSI: (liver weight/body weight)liver weight)·100] of cultured fish were significantly higher than those of wild fish. Females in the cultured group had significantly lower HSIs than males. The liver of both wild and cultured L. ferruginea was interspersed with pancreatic tissue. The main components of the liver tissue were irregular cords of hepatocytes arranged in tubules which surrounded vascular sinusoids. The hepatocytes contained an abundance of lipid, much of which appeared to be neutral lipids, in both sexes of the cultured fish. Total and neutral lipid droplets were larger, and the area occupied by these droplets was significantly greater in both cultured females and males compared to the wild fish, suggesting lipidosis in the cultured fish. In the cultured fish these differences were sex-dependent, the females having significantly more total and neutral lipids in hepatocytes than the males. This suggests a potentially greater storage capacity in females and/or a higher lipid metabolism in males. There were no statistically significant differences in glycogen content between the cultured and wild fish, or between the sexes in both sampling groups.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 55%

Structure of and energy reserves in the liver of wild and cultured yellowtail flounder, Limanda ferruginea

Ree

Spurrell

2003

Marine Biology

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“…PCP was shown to have adverse effects on reproduction of Japanese medaka (Zha et al, 2006) and is suspect of having long-term effects on ewe reproduction (Beard et al, 1999). Toxaphene studies on yellowtail flounder (Scott et al, 2002) indicate impact on liver function with possible adverse effect on reproduction, whereas Danio rerio was shown to be negatively impacted in its oviposition (FahraeusVanRee and Payne, 1997). Other authors investigated the effects of PCP and other phenolic compounds on aquatic invertebrates and showed that effects on metabolic rate are linked to the degree of narcotic toxicity.…”

Section: Discussionmentioning

confidence: 99%

Mode of Action Clustering of Chemicals and Environmental Samples on the Bases of Bacterial Stress Gene Inductions

Dardenne

Dongen

Nobels

et al. 2007

Toxicological Sciences

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Over the years, environment and the human population have seen an increasing exposure to both existing and newly developed chemicals. It is generally accepted that at least some of those are toxic, albeit as pure compound or in combination with others. In response to a growing public awareness and scientific data, the new European chemicals legislation (Registration, Evaluation and Authorization of Chemicals) is under implementation at the moment. As a consequence, during the coming years about 30,000 chemicals have to be assessed on their potential hazard for man and biota. Part of this assessment will be done using existing and new in vitro tests offering insight into the toxicity of chemicals and into their toxicological mode of action. This study presents data on a battery of 14 bacterial reporter gene assay allowing mode of action determination and statistical grouping of chemicals based on their induction profile. Gene induction results are used to group reference chemicals in a statistical cascade employing hierarchical tree and k-means clustering for initial grouping. Both complementary, yet mathematically different, algorithms are consequently confirmed by principal component analysis (PCA). The gene induction profiles of an environmental extract with documented in vivo effects and a chemical with limited toxicological are data available and projected in the PCA vector space. The projection allows correct mode of action grouping and indicates that effect predictions based on the known toxicological effects of the reference compounds can be made.

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“…Given that underestimation of total lipids by Iatroscan TLC-FID approaches appears general to both aquatic invertebrates and fishes, we recommend that this approach only be used to estimate lipids of individual classes, cautioning against the common practice of summing up these results to measure total lipids. Additionally, owing to the potential for Iatroscan TLC-FID to underestimate total lipids in ''fatty'' fishes, care also should be taken when using lipid values from previous studies that used Iatroscan TLC-FID to quantify total lipids (e.g., Scott et al 2002;Zhou et al 1995). Given the range of total lipid values spanned in this study, a comparison of total lipid levels in other studies with those in ours here should provide a good means to determine whether previous Iatroscan TLC-FID studies may indeed be biased low.…”

Section: Microcolorimetric Spv Methods Vs Iatroscan Tlc-fid Methodsmentioning

confidence: 99%

Comparison of three microquantity techniques for measuring total lipids in fish

Ludsin

Fanslow

et al. 2008

Can. J. Fish. Aquat. Sci.

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To measure lipids in juvenile and adult fishes, we refined three microquantity approaches (microgravimetric, microcolorimetric sulfophosphovanillan (SPV), and Iatroscan thin layer chromatography – flame ionization detection (TLC–FID)) that were originally developed to measure lipids in small aquatic invertebrates. We also evaluated their precision and comparability by quantifying the total lipid content of age-1+ walleye ( Sander vitreus ), yellow perch ( Perca flavescens ), and lake whitefish ( Coregonus clupeaformis ) collected in Lake Erie (US–Canada), Lake Michigan (USA), and Muskegon Lake (Michigan, USA). Our findings demonstrate that (i) microquantity approaches provide estimates of total lipids in juvenile and adult fishes similar to those of more traditional macroquantity approaches, (ii) the microcolorimetric SPV and microgravimetric approaches produce near identical estimates of total lipid content, and (iii) the Iatroscan TLC–FID approach underestimates total lipids relative to the other approaches for individuals with high lipid levels. Ultimately, our research makes available additional techniques for measuring total lipid content of fishes that are less expensive than traditional techniques, owing to a reduced need for large quantities of samples and solvents.

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Sex Differences in Hepatic Lipids of Toxaphene-Exposed Juvenile Yellowtail Flounder (Pleuronectes ferrugineus Storer)

Cited by 13 publications

References 20 publications

Structure of and energy reserves in the liver of wild and cultured yellowtail flounder, Limanda ferruginea

Structure of and energy reserves in the liver of wild and cultured yellowtail flounder, Limanda ferruginea

Mode of Action Clustering of Chemicals and Environmental Samples on the Bases of Bacterial Stress Gene Inductions

Comparison of three microquantity techniques for measuring total lipids in fish

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