Sex differences in cholinergic systems in the interpeduncular nucleus following nicotine exposure and withdrawal

Correa, Victor L.; Flores, Rodolfo; Carcoba, Luis M.; Arreguin, Montserrat C.; O’Dell, Laura E.

doi:10.1016/j.neuropharm.2019.107714

Cited by 27 publications

(25 citation statements)

References 45 publications

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“…The present study found that there were no sex differences in the physical signs of withdrawal, consistent with previous studies from our laboratory (Torres et al, 2013;Correa et al, 2019) and others using similar lighting conditions (Hamilton et al, 2010). Also, the physical signs of withdrawal do not appear to be influenced by ovarian hormones, as OVX rats displayed similar physical signs as compared to male and intact female rats.…”

Section: Discussionsupporting

confidence: 92%

Estradiol promotes and progesterone reduces anxiety-like behavior produced by nicotine withdrawal in rats

Flores

Cruz

Uribe

et al. 2019

Preprint

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The present study assessed sex differences and the role of ovarian hormones in the behavioral effects of nicotine withdrawal. Study 1 compared physical signs, anxiety-like behavior, and corticosterone levels in male, intact female, and ovariectomized (OVX) female rats during nicotine withdrawal. Estradiol (E2) and progesterone levels were also assessed in intact females that were tested during different phases of the 4-day estrous cycle. Study 2 assessed the role of ovarian hormones in withdrawal by comparing the same measures in OVX rats that received vehicle, E2, or E2+progesterone prior to testing. Briefly, rats received a sham surgery or an ovariectomy procedure. Fifteen days later, rats were prepared with a pump that delivered nicotine for 14 days. On the test day, rats received saline or the nicotinic receptor antagonist, mecamylamine to precipitate withdrawal. Physical signs and anxiety-like behavior were assessed on the elevated plus maze (EPM) and light-dark transfer (LDT) tests. During withdrawal, intact females displayed greater anxiety-like behavior and corticosterone levels as compared to male and OVX rats. Females tested in estrus (when E2 is relatively low) displayed less anxiety-like behavior and corticosterone versus all other phases. Anxiety-like behavior and corticosterone were positively correlated with E2 and negatively correlated with progesterone. Intact females displaying high E2/low progesterone displayed greater anxiety-like behavior and corticosterone as compared to females displaying low E2/high progesterone. Lastly, OVX-E2 rats displayed greater anxiety-like behavior than OVX-E2+progesterone rat. These data suggest that E2 promotes and progesterone reduces anxiety-like behavior produced by withdrawal.

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Section: Discussionsupporting

confidence: 92%

Estradiol promotes and progesterone reduces anxiety-like behavior produced by nicotine withdrawal in rats

Flores

Cruz

Uribe

et al. 2019

Preprint

Self Cite

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“…Consistent with this possibility, intracranial injection of the nAChR antagonist mecamylamine directly into the mHb or IPn, but not the cortex, VTA or hippocampus, of nicotine-dependent mice precipitated physical withdrawal signs (Salas et al, 2009;Zhao-Shea et al, 2013). Injection of mecamylamine infused into the IPn of nicotine-dependent rats precipitated physical signs of withdrawal and increased the expression of anxiety-related behaviors (Correa et al, 2019). Infusion of the α3β4* nAChR antagonist SR 16,584 into the IPn of nicotine-dependent mice similarly precipitated physical signs of nicotine withdrawal (Zhao-Shea et al, 2013).…”

Section: Nicotine-induced Adap Tations In the Mhb -Ipn Avoidan Ce Cirmentioning

confidence: 75%

Addiction‐related neuroadaptations following chronic nicotine exposure

Wills

Kenny

2021

Journal of Neurochemistry

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“…In particular, this effect may explain the differences found between day 1 and day 7 of exposure, as the animals became habituated to the passive vapor puffs across 7 consecutive sessions and thus were likely experiencing lower stress levels. Interestingly, a recent study has demonstrated that females exhibit an increase in α5 nicotinic receptor subunit expression in the interpeduncular nucleus following chronically administered nicotine, which was also correlated with anxiety-like behavior 50 . Thus, differential effects related to an interaction between nicotine modulation of brain function and anxiety-related behaviors may underlie the effects found in females.…”

Section: Discussionmentioning

confidence: 99%

Nicotine e‐cigarette vapor inhalation and self‐administration in a rodent model: Sex‐ and nicotine delivery‐specific effects on metabolism and behavior

et al. 2021

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E-cigarettes, which deliver vaporized nicotine, have dramatically risen in popularity in recent years, despite many unanswered questions about safety, efficacy in reducing dependence, and overall impact on public health. Other factors, such as sex, also play an important role in determining behavioral and neurochemical responses to drugs of abuse.In these studies, we sought to develop a protocol for vaporized e-cigarette nicotine self-administration in rats, as a foundation to better understand the differing effects of nicotine exposure routes on behavior and physiological function. We report a novel method that elicits robust nicotine vapor self-administration in male and female rats. Our findings indicate that 5 mg/ml nicotine vape solution provides a high level of consistency in lever-pressing behavior for both males and females.Moreover, in male rats, we find that such e-cigarette nicotine vapor induces similar blood levels of nicotine's main metabolite, cotinine, as that found with intravenous nicotine self-administration. Therefore, the breathing pattern during vapor exposure in males leads to similar levels of titrated nicotine intake as with intravenous nicotine self-administration. Interestingly, a differential effect was found in the females, in which the same conditions of vapor exposure led to decreased cotinine levels with vapor compared to intravenous self-administration. Finally, differences in nicotine-mediated locomotion provide further support of the physiological effects of e-cigarette vapor inhalation. Taken together, our findings reveal important sex differences in nicotine intake based on the route of 1 exposure, and we further establish a protocol for nicotine vapor selfadministration in rats.

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Sex differences in cholinergic systems in the interpeduncular nucleus following nicotine exposure and withdrawal

Cited by 27 publications

References 45 publications

Estradiol promotes and progesterone reduces anxiety-like behavior produced by nicotine withdrawal in rats

Estradiol promotes and progesterone reduces anxiety-like behavior produced by nicotine withdrawal in rats

Addiction‐related neuroadaptations following chronic nicotine exposure

Nicotine e‐cigarette vapor inhalation and self‐administration in a rodent model: Sex‐ and nicotine delivery‐specific effects on metabolism and behavior

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