Sex Determination Using RNA-Sequencing Analyses in Early Prenatal Pig Development

Teixeira, Susana Amaral; Ibelli, A. M. G.; Cantão, Maurício Egídio; Oliveira, Hellenn Cardoso; Ledur, Mônica Corrêa; Peixoto, J. de O.; Marques, Daniele Botelho Diniz; Costa, Karine Assis; Coutinho, Luiz Lehmann; Guimarães, Simone Eliza Facioni

doi:10.3390/genes10121010

Cited by 10 publications

(9 citation statements)

References 46 publications

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“…The procedures of RNA extraction, library preparation, and sequencing were performed as previously described in [ 14 ]. Briefly, 100 mg of whole macerated conceptuses were mixed to 1 mL Trizol ® reagent (Invitrogen, San Diego, CA, USA) and the Qiagen RNeasy ® Mini kit (Qiagen, Hilden, Germany) was used for total RNA isolation, following the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

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Transcription Landscape of the Early Developmental Biology in Pigs

Teixeira

Marques

Costa

et al. 2021

Animals

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Since pre- and postnatal development are programmed during early prenatal life, studies addressing the complete transcriptional landscape during organogenesis are needed. Therefore, we aimed to disentangle differentially expressed (DE) genes between fetuses (at 35 days old) and embryos (at 25 days old) through RNA-sequencing analysis using the pig as model. In total, 1705 genes were DE, including the top DE IBSP, COL6A6, HBE1, HBZ, HBB, and NEUROD6 genes, which are associated with developmental transition from embryos to fetuses, such as ossification, skeletal muscle development, extracellular matrix organization, cardiovascular system, erythrocyte differentiation, and neuronal system. In pathway analysis, embryonic development highlighted those mainly related to morphogenic signaling and cell interactions, which are crucial for transcriptional control during the establishment of the main organs in early prenatal development, while pathways related to myogenesis, neuronal development, and cardiac and striated muscle contraction were enriched for fetal development, according to the greater complexity of organs and body structures at this developmental stage. Our findings provide an exploratory and informative transcriptional landscape of pig organogenesis, which might contribute to further studies addressing specific developmental events in pigs and in other mammals.

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Section: Methodsmentioning

confidence: 99%

“…All these analyses were performed following the BAQCOM pipeline [ 18 ]. The conceptuses’ sexes were determined according to Teixeira et al [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

Transcription Landscape of the Early Developmental Biology in Pigs

Teixeira

Marques

Costa

et al. 2021

Animals

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“…Another study attested a significant change in two miRNAs, let-7 g and miR-221, which were overexpressed in the serum of individuals and more prominent in women. 65…”

Section: Sex Determinationmentioning

confidence: 99%

Application of Ribonucleic Acid (RNA) in Forensic Sciences

Jawad¹,

Noreen²,

Ashraf³

et al. 2020

BIOMEDICA

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Forensic science has been helping law enforcement agencies in better understanding and presenting the evidence in the court of law. In certain situations, when conventional forensic methods of investigations cannot make better conclusions with more specific accuracy, then molecular techniques do help in reaching the acquired accuracy in the results regarding the identification of evidence. Advanced molecular techniques, which are using Deoxyribose Nucleic acid (DNA), Ribose Nucleic Acid (RNA), and protein molecules to produce forensically important information from the samples recovered at the crime scene. DNA can only distinguish among individuals but is unable to discriminate the type of samples originated from the same sample. For this RNA has become a molecule of interest for its different levels of expression in different cells/tissues of an individual. RNA molecules of different types are being used to build up models for several purposes (injury-age, new-born age, molecular cause of death, etc.). Modern techniques like Real-time Polymerase Chain Reaction (PCR) and Microarray are being used for the detection of RNA molecules of interest both in the form of its abundance and as a unique molecular detection. This script will help in understanding the importance of RNA application in forensic sciences by providing an overview of the research done to date and the techniques being used for this purpose.

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“…RNA sequencing (RNA-seq) is a high through-put technology that can be employed to detect quantitative gene expression and to analyze different expression pro les at the whole transcriptome level [6]. With the enhanced sensitivity and declining cost, RNA-seq has been widely used in livestock and poultry research [7][8]. Because the spleen is one of the dominating sites for priming of the primary immune responses, in the present study, transcriptome sequencing was performed to investigate the effect of β-glucan on gene expression in chicken spleen and to explore the potential signal transduction pathways.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of spleen in chickens administered β-glucan derived from yeast cell wall

Cao

Zhang

Huang

et al. 2022

Preprint

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Background Our previous study shown that oral administration of a product contained yeast cell wall polysaccharides enhanced immune responses elicited by Newcastle disease virus and changed microbial community of cecum in chickens. Results The present study was design to investigate the potential molecular mechanism in relation to the immunomodulation of β-glucan in chickens. Using RNA-sequence (RNA-seq) technique, we identified 198 differential expression genes (DEGs) in spleen in chickens after oral administration of β-glucan. In addition, these DEGs were significantly enriched in 205 Gene Ontology (GO) terms and 7 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Conclusions β-glucan might regulate chicken immune system by regulating expression of genes involved in cognition, cytokines, binding, enzyme activities and multiple signaling pathway.

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Sex Determination Using RNA-Sequencing Analyses in Early Prenatal Pig Development

Cited by 10 publications

References 46 publications

Transcription Landscape of the Early Developmental Biology in Pigs

Transcription Landscape of the Early Developmental Biology in Pigs

Application of Ribonucleic Acid (RNA) in Forensic Sciences

Transcriptome analysis of spleen in chickens administered β-glucan derived from yeast cell wall

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