Sex Chromosomes Are Severely Disrupted in Gastric Cancer Cell Lines

Oh, Seungjong; Min, Kyoungmi; Kim, Myungshin; Lee, Suk Kyeong

doi:10.3390/ijms21134598

Cited by 4 publications

(3 citation statements)

References 36 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, cell lines tend to continuously mutate as the passage number increases during cell culture. A previous study indicated that a loss of Y chromosome accompanied with X chromosome duplication in three of six male gastric cancer cell lines tested 27 . Also two of six female gastric cancer cell lines showed a loss of one X chromosome.…”

Section: Discussionmentioning

confidence: 94%

“…A previous study indicated that a loss of Y chromosome accompanied with X chromosome duplication in three of six male gastric cancer cell lines tested. 27 Also two of six female gastric cancer cell lines showed a loss of one X chromosome. These results indicate that cells isolated from male or female tissues may not fully represent the sex characteristics of the donors especially when those cells are immortalized.…”

Section: F I G U R Ementioning

confidence: 98%

See 1 more Smart Citation

Sex reporting of cells used in cancer research: A systematic review

Park,

Kim,

Choi

et al. 2024

The FASEB Journal

Self Cite

View full text Add to dashboard Cite

Sex and gender disparities in biomedical research have been emphasized to improve scientific knowledge applied for the health of both men and women. Despite sex differences in cancer incidence, prognosis, and responses to therapeutic agents, mechanistic explanations at molecular levels are far from enough. Recent studies suggested that cell sex is an important biological variable due to differences in sex chromosome gene expression and differences in events associated with developmental biology. The objective of this study was to analyze the reporting of sex of cells used in cancer research using articles published in Cancer Cell, Molecular Cancer, Journal of Hematology & Oncology, Journal for ImmunoTherapy of Cancer, and Cancer Research in 2020, and to examine whether there exists any sex bias. We found that the percentage of cells with sex notation in the article was 36.5%. Primary cells exhibited higher sex notation compared to cell lines. A higher percentage of female cells were used in cell cultures with sex notation. Also, sex‐common cells omitted sex description more often compared to sex‐specific cells. None of the cells isolated from embryo and esophagus reported the cell sex in the article. Our results indicate cell sex report in cancer research is limited to a small proportion of cells used in the study. These results call for acknowledging the sex of cells to increase the applicability of biomedical research discoveries.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: F I G U R Ementioning

confidence: 98%

Sex reporting of cells used in cancer research: A systematic review

Park,

Kim,

Choi

et al. 2024

The FASEB Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…The clean reads were then mapped to the human genome (GRCh38_Ensembl104) using the Hisat2 [40]. HTseq was used to obtain the gene counts, and the FPKM method was used to determine the gene expression [41].…”

Section: Rna Sequencingmentioning

confidence: 99%

Ploidy Status of Ovarian Cancer Cell Lines and Their Association with Gene Expression Profiles

Zhang

Liu

et al. 2023

Biomolecules

View full text Add to dashboard Cite

As a cancer type potentially dominated by copy number variations, ovarian cancer shows hyperploid karyotypes and large-scale chromosome alterations, which might be promising biomarkers correlated with tumor metastasis and chemoresistance. Experimental studies have provided more information about the roles of aneuploids and polyploids in ovarian cancer. However, ploidy evaluation of ovarian cancer cell lines is still limited, even in some ploidy-related research. Herein, the ploidy landscape of 51 ovarian cancer cell lines from the Cancer Cell Line Encyclopedia (CCLE) were analyzed, and the ploidy statuses of 13 human ovarian cancer cell lines and 2 murine cell lines were evaluated using G-banding and flow cytometry. Most human ovarian cancer cell lines were aneuploid, with modal numbers of 52–86 and numerical complexity ranging from 5 to 12. A2780, COV434 and TOV21G were screened as diploid cell lines, with a modal number of 46, a low aneuploid score and a near-diploid ploidy value. Two murine cell lines, both OV2944-HM1 and ID-8, were near-tetraploid. Integrated information on karyotypes, aneuploid score and ploidy value supplied references for a nondiploid model construction and a parallel analysis of diploid versus aneuploid. Moreover, the gene expression profiles were compared between diploid and aneuploid cell lines. The functions of differentially expressed genes were mainly enriched in terms of protein function regulation, TGF-β signaling and cell adhesion molecules. Genes downregulated in the aneuploid group were mainly related to metabolism and protein function regulation, and genes upregulated in the aneuploid group were mainly involved in immune regulation. Differentially expressed genes were randomly distributed on all chromosomes, while chromosome 1 alteration might contribute to immune-related alterations in aneuploid cell lines. Chromosome 19 alteration might be potentially significant for aneuploid ovarian cancer cell lines and patients, which needs further verification in ploidy research.

show abstract

Sex omission and male bias are still widespread in cell experiments

Kim

Min

Paik

et al. 2021

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

Integrating sex as an important biological variable is imperative to enhance the accuracy and reproducibility of cell-based studies, which provide basic information for subsequent preclinical and clinical study designs. Recently, international funding agencies and renowned journals have been attempting to integrate sex as a variable in every research step. To understand what progress has been made in reporting of cell sex in the articles published in AJP-Cell Physiology since the analysis in 2013, we examined the sex notation of the cells in relevant articles published in the same journal in 2018. Of the 107 articles reporting cell experiments, 53 reported the sex of the cells, 18 used both male and female cells, 23 used male cells only, and 12 used female cells only. Sex omission was more frequent when cell lines were used than when primary cells were used. In the articles describing experiments performed using rodent primary cells, more than half of the studies used only male cells. Our results showed an overall improvement in sex reporting for cells in AJP-Cell Physiology articles from 2013 (25%) to 2018 (50%). However, sex omission and male bias were often found still. Furthermore, the obtained results were rarely analyzed by sex even when both male and female cells were used in the experiments. To boost sex considerate research implementation in basic biomedical studies, cooperative efforts of the research community, funders, and publishers are urged.

show abstract

Sex Chromosomes Are Severely Disrupted in Gastric Cancer Cell Lines

Cited by 4 publications

References 36 publications

Sex reporting of cells used in cancer research: A systematic review

Sex reporting of cells used in cancer research: A systematic review

Ploidy Status of Ovarian Cancer Cell Lines and Their Association with Gene Expression Profiles

Sex omission and male bias are still widespread in cell experiments

Contact Info

Product

Resources

About