Sex-Biased Gene Expression and Isoform Profile of Brine Shrimp Artemia franciscana by Transcriptome Analysis

Jo, Euna; Lee, Seung-Jae; Choi, Eun‐Kyung; Kim, Jinmu; Lee, Jun-Hyuck; Park, Hyun

doi:10.3390/ani11092630

Cited by 6 publications

(3 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two different A. franciscana de novo transcriptome assemblies were made using Trinity. The first using pooled RNA-seq reads from male heads and testes [2 replicates each ( Huylmans et al 2019 )], and the second using the published whole-body male RNA-seq library (SRR14598203, Jo, Lee, Choi, Kim, Lee, and Park 2021 ). The 2 assemblies were concatenated and then filtered using the tr2aacds.pl script from EvidentialGene, and mapped to the A. sinica genome using the same procedure as described in section Mapping of W candidates to the A. sinica genome .…”

Section: Methodsmentioning

confidence: 99%

ZW sex-chromosome evolution and contagious parthenogenesis in Artemia brine shrimp

et al. 2022

View full text Add to dashboard Cite

Eurasian brine shrimp (genus Artemia) have closely related sexual and asexual lineages of parthenogenetic females, which produce rare males at low frequencies. Although they are known to have ZW chromosomes, these are not well characterized, and it is unclear whether they are shared across the clade. Furthermore, the underlying genetic architecture of the transmission of asexuality, which can occur when rare males mate with closely related sexual females, is not well understood. We produced a chromosome-level assembly for the sexual Eurasian species A. sinica and characterized in detail the pair of sex chromosomes of this species. We combined this new assembly with short-read genomic data for the sexual species A. sp. Kazakhstan and several asexual lineages of A. parthenogenetica, allowing us to perform an in-depth characterization of sex-chromosome evolution across the genus. We identified a small differentiated region of the ZW pair that is shared by all sexual and asexual lineages, supporting the shared ancestry of the sex chromosomes. We also inferred that recombination suppression has spread to larger sections of the chromosome independently in the American and Eurasian lineages. Finally, we took advantage of a rare male, which we backcrossed to sexual females, to explore the genetic basis of asexuality. Our results suggest that parthenogenesis is likely partly controlled by a locus on the Z chromosome, highlighting the interplay between sex determination and asexuality.

show abstract

Section: Methodsmentioning

confidence: 99%

ZW sex-chromosome evolution and contagious parthenogenesis in Artemia brine shrimp

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The first using pooled RNA-seq reads from male heads and testes (two replicates each, Huylmans et al . 2019), and the second using the published whole-body male RNA-seq library (SRR14598203, Jo et al . 2021b).…”

Section: Methodsmentioning

confidence: 99%

ZW sex-chromosome evolution and contagious parthenogenesis in Artemia brine shrimp

Elkrewi

Khauratovich

Toups

et al. 2022

Preprint

View full text Add to dashboard Cite

Eurasian brine shrimp (genus Artemia) have closely related sexual and asexual lineages of parthenogenetic females, which produce rare males at low frequencies. Although they are known to have ZW chromosomes, these are not well characterized, and it is unclear whether they are shared across the clade. Furthermore, the underlying genetic architecture of the transmission of asexuality, which can occur when rare males mate with closely related sexual females, is not well understood. We produced a chromosome-level assembly for the Eurasian species A. sinica and characterized in detail the pair of sex chromosomes of this species. We combined this with short-read genomic data for the sexual species A. sp. Kazakhstan and several lineages of A. parthenogenetica, allowing us to perform a first in-depth characterization of sex-chromosome evolution across the genus. We identified a small differentiated region of the ZW pair that is shared by all sexual and asexual lineages, supporting the shared ancestry of the sex chromosomes. We also inferred that recombination suppression has spread to larger sections of the chromosome independently in the American and Eurasian lineages. Finally, we took advantage of a rare male, which we backcrossed to sexual females, to explore the genetic basis of asexuality. Our results suggest that parthenogenesis may be partly controlled by a locus on the Z chromosome, highlighting the interplay between sex determination and asexuality.

show abstract

“…Eight sex-linked AFLP marker alleles have been reported in A. franciscana that are inherited from the female parent [ 1 ]. Some genes related to sexual differentiation in A. franciscana have been identified and shown as sex-biased gene expression [ 8 ]. Masculinizer gene ( Ar-Masc) was identified in A. franciscana [ 9 ], the MASC protein encoded in the Z chromosome activates a sex-determining gene that regulates gene splicing of Doublesex ( dsx ) into the male-specific isoform in the silkworm Bombyx mori [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

The Sex-Specific Splicing of Doublesex in Brine Shrimp Artemia franciscana

Viet

Christiaens

Vos

et al. 2022

Genes

View full text Add to dashboard Cite

The understanding of sex determination and differentiation in animals has recently made remarkable strides through the use of advanced research tools. At the gene level, the Mab-3-related transcription factor (Dmrt) gene family, which encodes for the typical DNA-binding doublesex/Mab-3 (DM) domain in their protein, is known for its contribution to sex determination and differentiation in insects. In this study, DNA-binding DM domain screening has identified eight transcripts from Artemia franciscana transcriptomic that encode proteins containing one conserved DNA-binding DM domain. The genome mapping confirmed that these eight transcripts are transcribed from six different loci on the A. franciscana genome assembly. One of those loci, the Af.dsx-4 locus, is closely related to Doublesex, a gene belonging to the Dmrt gene family. This locus could be transcribed into three alternative transcripts, namely Af.dsx4, Af.dsxF and Af.dsxM. While Af.dsx4 and Af.dsxF could putatively be translated to form an identical Af.dsxF protein of 186 aa long, Af.dsxM translates for an Af.dsxM protein of 289 aa long but shares a DNA-binding DM domain. Interestingly, Af.dsxF and Af.dsxM are confirmed as sex-specific transcripts, Af.dsxF is only present in females, and Af.dsxM is only present in male individuals. The results suggest that the sex-specific splicing mechanism of the doublesex described in insects is also present in A. franciscana. Af.dxs-4 locus can be used in further studies to clarify the sex determination pathways in A. fracnciscana.

show abstract

Sex-Biased Gene Expression and Isoform Profile of Brine Shrimp Artemia franciscana by Transcriptome Analysis

Cited by 6 publications

References 73 publications

ZW sex-chromosome evolution and contagious parthenogenesis in Artemia brine shrimp

ZW sex-chromosome evolution and contagious parthenogenesis in Artemia brine shrimp

ZW sex-chromosome evolution and contagious parthenogenesis in Artemia brine shrimp

The Sex-Specific Splicing of Doublesex in Brine Shrimp Artemia franciscana

Contact Info

Product

Resources

About