Sex- and tissue-specific expression of P450 aromatase (cyp19a1a) in the yellowtail clownfish, Amphiprion clarkii

Histological Observation on Adult Gonads from Meiogynogentic Olive Flounder Paralichthys olivaceus

“…In the present research, mammal antibody of P450arom was used to further check developmental status of gonad in lower vertebrates including fish by means of immunohistochemical localization, which has been validated available as the result of its high conservation. The positive P450arom responses were detected in ovarian follicles of these studied ovaries and their intensity was close to that of control flounder, which was similar with the locations in ovaries of yellowtail clownfish (Kobayashi et al, 2010). Comprehensively considering the above described results and the document of viable offsprings successfully produced by meiotic gynogenetic flounder broodstock (Jiang et al, 2017), it is reasonable to suggest that the mature meiogynogenetic ovaries of olive flounders could have the potencies of normal reproductive processes to produce sufficient estrogen, go through normal ovulation and spawn good eggs upon artificial treatment, even for these with malformed ovaries (Felip et al, 2002), as following of meiogynogenetic diploids induced from cyprinid loach Misgurnus anguillicaudatus (Suzuki et al, 1985), Thai walking catfish Clarias macrocephalus (Na-Nakorn, 1995), sea bass Dicentrarchus labrax (Francescon et al, 2005), and turbot Scophthalmus maximus (Cal et al, 2006).…”

Clownfish

“…In A. melanopus, Kiss appeared to stimulate gonadotropin-releasing hormone (GnRH) release as well as the production of various steroid hormones including estradiol-17β (E2) and 11ketotestosterone (11-KT), thus playing a central role in the maturation of the clownfish (Kim et al, 2014). The level of gonadal aromatase (cyp19a1a) is significantly lower in ambisexual gonad (male) of A. clarkii than in females (Kobayashi et al, 2010). For example in A. clarkii, a clear decrease in E2 and an increase of 11-KT is observed during testicular differentiation (Miura et al, 2008(Miura et al, , 2013.…”

AreAcartia tonsacold-stored eggs a suitable food source for the marine ornamental speciesAmphiprion polymnus? A feeding study

“…; Kobayashi et al . ). At date, most of the marine fish species are still reared using rotifers ( B. plicatilis , B. rotundiformis ) and Artemia nauplii but unfortunately, using rotifers and Artemia during this early period in life history does not always promote optimal larval growth because these live prey may contain an inadequate fatty acid profile (Estévez et al .…”

“…Among marine fish models, clownfishes (Amphiprion spp.) may be considered good candidates because they are protandrous hermaphrodites, they regularly spawn in the laboratory (Avella et al 2007(Avella et al , 2010, larval development is extremely short (Olivotto et al 2010), they are not able to synthesize HUFAs de novo and, finally, their genome is partially available in Genbank (Olivotto et al 2009;Kim et al 2010;Kobayashi et al 2010). At date, most of the marine fish species are still reared using rotifers (B. plicatilis, B. rotundiformis) and Artemia nauplii but unfortunately, using rotifers and Artemia during this early period in life history does not always promote optimal larval growth because these live prey may contain an inadequate fatty acid profile (Este´vez et al 1999;Sargent et al 1999;Holt 2003;Olivotto et al 2003;Faulk & Holt 2005).…”