Sex and the single cell: meiosis in yeast.

Roeder, G. Shirleen

doi:10.1073/pnas.92.23.10450

Cited by 125 publications

(95 citation statements)

References 88 publications

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“…Nevertheless, the authors were able to evaluate the length of the conversion tract in the five recombinant individuals carrying the parental flanking markers and to pinpoint both recombination junctions in two of them. In both cases, Dooner and Martínez-Férez estimate the length of the conversion tract to be between 1 and 1.5 kb, which correlates with reported lengths of conversion tracts in yeast (reviewed in Roeder, 1995).…”

Section: In Thls Issuesupporting

confidence: 71%

“…In the future, it should be possible to evaluate these features to determine whether andlor how the molecular mechanisms of recombination are conserved between plants and other eukaryotes. For example, a potential link between transcription (and/ or transcription factors) and recombination has been proposed in yeast (Roeder, 1995). 1s this also true in plants?…”

Section: In Thls Issuementioning

confidence: 99%

“…In this organism, at least 10 genes that play a role in the initiation, propagation, and/or resolution of meiotic recombination events have been identified. lnteractions among a number of the corresponding proteins have been elucidated, and some useful predictions of the possible sequence of events during recombination in yeast have been proposed (e.g., Lichten and Goldman, 1995;Roeder, 1995;Kleckner, 1996;Haber, 1997).…”

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confidence: 99%

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Meiotic Recombination: Casting for Clues in Bronze

Taylor¹

1997

Plant Cell

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Section: In Thls Issuesupporting

confidence: 71%

Section: In Thls Issuementioning

confidence: 99%

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confidence: 99%

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Meiotic Recombination: Casting for Clues in Bronze

Taylor¹

1997

Plant Cell

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“…Defects either in the initiation or the processing of recombination intermediates have profound consequences on the output of meiosis: they can stop meiotic cell cycle progression and/or induce missegregation of the homologues leading to inviable products (Rockmill & Roeder 1988;Hollingsworth et al 1990;Roeder 1995). Sequence divergence can also have profound effects on meiosis.…”

Section: Introductionmentioning

confidence: 99%

Sensing of DNA non‐homology lowers the initiation of meiotic recomination in yeast

Rocco

Nicolas

1996

Genes to Cells

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Background: Meiotic recombination between homologous chromosomes in the yeast Saccharo-myces cerevisiae is initiated by the formation of DNA double-strand breaks (DSBs). The mechanism of DSB formation and the factors that determine their frequency and location have yet to be elucidated. Current studies of meiotic recombination are also concerned with the question of the functional relationship between DSB formation and the other meiotic processes of homology searching, pairing and synapsis of homologues.

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“…The choice between crossover and noncrossover is tightly regulated in both mitotic and meiotic cells. In meiotic S. cerevisiae cells the correct number of crossovers are required for proper chromosome segregation; the proportion of gene conversion accompanied by crossing over varies between 25% and 50% depending on the locus (Roeder 1995). In mitotic cells, the proportion of crossovers is much lower, ranging between <1% and 15%, depending on the recombination assay and organism (Esposito 1978;Nassif et al 1994;Johnson and Jasin 2000;Virgin et al 2001;Stark and Jasin 2003).…”

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confidence: 99%

Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination

Prakash¹,

Satory²,

Dray³

et al. 2009

Genes Dev.

232

342

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Eukaryotes possess mechanisms to limit crossing over during mitotic homologous recombination, thus avoiding possible chromosomal rearrangements. We show here that budding yeast Mph1, an ortholog of human FancM helicase, utilizes its helicase activity to suppress spontaneous unequal sister chromatid exchanges and DNA double-strand break-induced chromosome crossovers. Since the efficiency and kinetics of break repair are unaffected, Mph1 appears to channel repair intermediates into a noncrossover pathway. Importantly, Mph1 works independently of two other helicases-Srs2 and Sgs1-that also attenuate crossing over. By chromatin immunoprecipitation, we find targeting of Mph1 to double-strand breaks in cells. Purified Mph1 binds D-loop structures and is particularly adept at unwinding these structures. Importantly, Mph1, but not a helicase-defective variant, dissociates Rad51-made D-loops. Overall, the results from our analyses suggest a new role of Mph1 in promoting the noncrossover repair of DNA double-strand breaks.[Keywords: Genome instability; recombination; DNA helicase; crossing over; Fanconi anemia] Supplemental material is available at http://www.genesdev.org. Received September 8, 2008; revised version accepted November 12, 2008. DNA double-strand breaks (DSBs) that arise during DNA replication or are induced by DNA damaging agents, such as ionizing radiation, are frequently repaired by homologous recombination (HR). In yeast and other eukaryotes, the RAD52 epistasis group of proteins mediate homologous recombination (for reviews, see Paques and Haber 1999;Krogh and Symington 2004). In this process, DSB ends are resected by nucleases to create 39 ssDNA that becomes coated with the recombinase protein Rad51. The Rad51-ssDNA nucleoprotein filament then carries out a search for a homologous donor DNA sequence and promotes strand invasion of the donor molecule to form a D-loop (Sung and Klein 2006). After D-loop formation, there appear to be two alternative pathways that result in DSB repair. One pathway involves the formation of a double Holliday junction (dHJ) that can be resolved by symmetrical strand cleavage into either a crossover or noncrossover gene conversion (Szostak et al. 1983). An alternative mechanism, called synthesis-dependent strand annealing (SDSA), posits formation mostly of noncrossovers, and in most cases does not involve a dHJ intermediate (for review, see Paques and Haber 1999). In support of the SDSA model of gene conversion, we showed recently that both newly synthesized strands are inherited by the broken recipient DNA molecule (Ira et al. 2006). The choice between crossover and noncrossover is tightly regulated in both mitotic and meiotic cells. In meiotic S. cerevisiae cells the correct number of crossovers are required for proper chromosome segregation; the proportion of gene conversion accompanied by crossing over varies between 25% and 50% depending on the locus (Roeder 1995). In mitotic cells, the proportion of crossovers is much lower, ranging between <1% and 7 These authors c...

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Sex and the single cell: meiosis in yeast.

Abstract: Recent studies of Saccharomyces cerevisiae have significantly advanced our understanding of the molecular mechanisms of meiotic chromosome behavior. Structural

Cited by 125 publications

References 88 publications

Meiotic Recombination: Casting for Clues in Bronze

Meiotic Recombination: Casting for Clues in Bronze

Sensing of DNA non‐homology lowers the initiation of meiotic recomination in yeast

Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination

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