Sex and species differences in plasma oxytocin using an enzyme immunoassay

Kramer, Kristin M.; Cushing, Bruce S.; Carter, C Sue; Wu, Julie M.; Ottinger, Mary Ann

doi:10.1139/z04-098

Cited by 94 publications

(75 citation statements)

References 51 publications

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“…Samples were assayed in duplicate; the intra-assay and inter-assay coefficients of variation were under 10%. The assay has been validated (Carter et al, 2007;Kramer, Cushing, Carter, Wu, & Ottinger, 2004), and this protocol has been used extensively (Ebstein, Knafo, Mankuta, Chew, & Lai, 2012) with stable individual values (Gouin et al, 2010;Weisman, Zagoory-Sharon, Schneiderman, Gordon, & Feldman, 2013). The OT values in the present sample fall within the range reported in other samples of pregnant women using this protocol (Prévost et al, 2014).…”

Section: Methodssupporting

confidence: 67%

Attachment security and recent stressful life events predict oxytocin levels: a pilot study of pregnant women with high levels of cumulative psychosocial adversity

Samuel

Hayton

Gold

et al. 2015

Attachment & Human Development

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Lower OT levels were found among those who were insecurely attached, and among those who experienced SLE within the last year. In a multiple linear regression, both attachment security and SLE significantly contributed to a model of prenatal OT levels. These individual difference factors explained 38% of the variance in prenatal OT, which may in turn predict poorer maternal mental health and caregiving outcomes during the postpartum period.

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Section: Methodssupporting

confidence: 67%

Attachment security and recent stressful life events predict oxytocin levels: a pilot study of pregnant women with high levels of cumulative psychosocial adversity

Samuel

Hayton

Gold

et al. 2015

Attachment & Human Development

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show abstract

“…2.1.5 Circulating Hormone and Peptide Analysis-Plasma levels of oxytocin were determined using a commercially available enzyme-linked immunosorbent assay kit (Assay Designs, Ann Arbor, MI), which has been validated previously for use in prairie voles (Kramer et al, 2004). Inter-and intra-assay coefficients of variation for oxytocin are 10.0% and 10.5%, respectively.…”

Section: 14mentioning

confidence: 99%

Social isolation induces behavioral and neuroendocrine disturbances relevant to depression in female and male prairie voles

Grippo

Gerena

Huang

et al. 2007

Psychoneuroendocrinology

299

279

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SummarySupportive social interactions may be protective against stressors and certain mental and physical illness, while social isolation may be a powerful stressor. Prairie voles are socially monogamous rodents that model some of the behavioral and physiological traits displayed by humans, including sensitivity to social isolation. Neuroendocrine and behavioral parameters, selected for their relevance to stress and depression, were measured in adult female and male prairie voles following 4 weeks of social isolation versus paired housing. In Experiment 1, oxytocin-immunoreactive cell density was higher in the hypothalamic paraventricular nucleus (PVN) and plasma oxytocin was elevated in isolated females, but not males. In Experiment 2, sucrose intake, used as an operational definition of hedonia, was reduced in both sexes following 4 weeks of isolation. Animals then received a residentintruder test, and were sacrificed either 10 minutes later for the analysis of circulating hormones and peptides, or two hours later to examine neural activation, indexed by c-Fos expression in PVN cells immunoreactive for oxytocin or corticotropin-releasing factor (CRF). Compared to paired animals, plasma oxytocin, ACTH and corticosterone were elevated in isolated females and plasma oxytocin was elevated in isolated males, following the resident-intruder test. The proportion of cells doublelabeled for c-Fos and oxytocin or c-Fos and CRF was elevated in isolated females, and the proportion of cells double-labeled for c-Fos and oxytocin was elevated in isolated males following this test. These findings suggest that social isolation induces behavioral and neuroendocrine responses relevant to depression in male and female prairie voles, although neuroendocrine responses in females may be especially sensitive to isolation.

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“…We note that the range of OT levels that was measured in the current study is according to the levels observed for Sprague-Dawely rats, measured by enzyme-linked immunoassay. 32 Leptin. Samples were diluted 1:11 in the assay buffer and treated according to the instructions of the commercial kit.…”

Section: Tissue Collectionsmentioning

confidence: 99%

Adaptation to lactation in OLETF rats lacking CCK-1 receptors: body weight, fat tissues, leptin and oxytocin

et al. 2008

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Objective: To understand the adaptation to lactation of obese rats, by studying the interplay among the gut hormone cholecystokinin (CCK), the adiposity hormone leptin and the affiliation hormone oxytocin in modulating body mass and fat storage. Design: Strain differences were examined between Otsuka Long Evans Tokushima Fatty (OLETF) rats lacking expression of functional CCK-1 receptors and Long Evans Tokushima Otsuka (LETO) controls, tested as nulliparous dams, at the 7 and 15th lactation day, at weaning (lactation day 22) or 8 weeks postweaning. Measurements: We measured body mass, fat pads (brown, retroperitoneal and inguinal) and inguinal adipocytes. Plasma levels of leptin and oxytocin were determined. Results: Fat depots of LETO female rats were larger during lactation compared to the levels found in postweaning and nulliparous female rats. LETO female rats gained weight and accumulated fat during pregnancy and lactation, returning to their normal fat levels postweaning. In contrast, OLETF female rats presented lower body weight and fat depots during the lactation period than nulliparous dams, and regained the weight and fat postweaning. Plasma leptin and oxytocin were highly correlated and followed the same pattern. OLETF leptin levels were highly correlated with fat depot and inguinal cell surface. No significant correlation was found for LETO parameters. Conclusions: Pregnancy and lactation are energy-consuming events, which naturally induce female rats to increase food intake and accumulate fat. When challenged by the demands of rapidly growing preobese OLETF pups, OLETF dams' fat stores are reduced to lean, LETO levels. During lactation, sensitivity of the oxytocinergic neurons descending from the paraventricular nuclei to the nucleus of the solitary tract to CCK is reduced. We theorized that this pathway is not available to OLETF female rats that lack functional CCK-1 receptors to mediate the signal. The current study contributes to the understanding of the female body's adaptation to lactation.

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Sex and species differences in plasma oxytocin using an enzyme immunoassay

Cited by 94 publications

References 51 publications

Attachment security and recent stressful life events predict oxytocin levels: a pilot study of pregnant women with high levels of cumulative psychosocial adversity

Attachment security and recent stressful life events predict oxytocin levels: a pilot study of pregnant women with high levels of cumulative psychosocial adversity

Social isolation induces behavioral and neuroendocrine disturbances relevant to depression in female and male prairie voles

Adaptation to lactation in OLETF rats lacking CCK-1 receptors: body weight, fat tissues, leptin and oxytocin

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