Sewage as a rich source of phage study against Pseudomonas aeruginosa PAO

Reza, Azizian; Nasser, Ahmad; Askari, Hasan; Kalani, Morovat Taheri; Sadeghifard, Nourkhoda; Pakzad, Iraj; Amini, Razieh; Nejad, Amir Sasan Mozaffari; Jalilian, Farid Azizi

doi:10.1016/j.biologicals.2015.05.004

Cited by 14 publications

(10 citation statements)

References 18 publications

(16 reference statements)

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“…However, since drug-resistant bacteria have become increasingly problematic and challenging, phage therapy is considered to be an important candidate for alternative therapy 12. Bacteriophages are viruses that are isolated from several ecological specimens including sewage, soil, and water 13–15. The isolation of bacteriophage from an environmental sample revealed that bacteriophages did not exert adverse effects on individuals 15–17.…”

Section: Phage Therapymentioning

confidence: 99%

“…Bacteriophages are viruses that are isolated from several ecological specimens including sewage, soil, and water 13–15. The isolation of bacteriophage from an environmental sample revealed that bacteriophages did not exert adverse effects on individuals 15–17. It is estimated that there are more than 4200 bacteriophages that exactly infect Mycobacterium spp; it is expected that 1025 phage launches a new infection cycle in every second of the day 18.…”

Section: Phage Therapymentioning

confidence: 99%

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<p>Phage therapy as a renewed therapeutic approach to mycobacterial infections: a comprehensive review</p>

Azimi¹,

Mosadegh²,

Nasiri³

et al. 2019

IDR

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Mycobacterial infections are considered to a serious challenge of medicine, and the emergence of MDR and XDR tuberculosis is a serious public health problem. Tuberculosis can cause high morbidity and mortality around the world, particularly in developing countries. The emergence of drug-resistant Mycobacterium infection following limited therapeutic technologies coupled with the serious worldwide tuberculosis epidemic has adversely affected control programs, thus necessitating the study of the role bacteriophages in the treatment of mycobacterial infection. Bacteriophages are viruses that are isolated from several ecological specimens and do not exert adverse effects on patients. Phage therapy can be considered as a significant alternative to antibiotics for treating MDR and XDR mycobacterial infections. The useful ability of bacteriophages to kill Mycobacterium spp has been explored by numerous research studies that have attempted to investigate the phage therapy as a novel therapeutic/diagnosis approach to mycobacterial infections. However, there are restricted data about phage therapy for treating mycobacterial infections. This review presents comprehensive data about phage therapy in the treatment of mycobacterial infection, specifically tuberculosis disease.

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Section: Phage Therapymentioning

confidence: 99%

Section: Phage Therapymentioning

confidence: 99%

<p>Phage therapy as a renewed therapeutic approach to mycobacterial infections: a comprehensive review</p>

Azimi¹,

Mosadegh²,

Nasiri³

et al. 2019

IDR

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“…In order to narrow down possible phage reservoirs when selecting phages to target MDR P. aeruginosa , clinically useful phages have been sourced from sampling sewage water adjacent to hospitals, as the phages found in abundance among human pathogenic bacteria are likely to use those target pathogens as hosts. As expected, phages successfully selected and isolated from sewage were shown in vitro to have a high efficacy against a variety of clinical and general laboratory strains of P. aeruginosa [ 65 ]. Sampled phages can be directly identified via the double drop agar method, assuming the titer is high enough or amplified for ease of identification [ 66 ].…”

Section: Phage Selection For Mdr Pseudomonas Aeruginosa Infectionsmentioning

confidence: 77%

Clinical Pharmacology of Bacteriophage Therapy: A Focus on Multidrug-Resistant Pseudomonas aeruginosa Infections

et al. 2021

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Pseudomonas aeruginosa is one of the most common causes of healthcare-associated diseases and is among the top three priority pathogens listed by the World Health Organization (WHO). This Gram-negative pathogen is especially difficult to eradicate because it displays high intrinsic and acquired resistance to many antibiotics. In addition, growing concerns regarding the scarcity of antibiotics against multidrug-resistant (MDR) and extensively drug-resistant (XDR) P. aeruginosa infections necessitate alternative therapies. Bacteriophages, or phages, are viruses that target and infect bacterial cells, and they represent a promising candidate for combatting MDR infections. The aim of this review was to highlight the clinical pharmacology considerations of phage therapy, such as pharmacokinetics, formulation, and dosing, while addressing several challenges associated with phage therapeutics for MDR P. aeruginosa infections. Further studies assessing phage pharmacokinetics and pharmacodynamics will help to guide interested clinicians and phage researchers towards greater success with phage therapy for MDR P. aeruginosa infections.

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“…Bacteriophage isolated from wastewater has shown its e ciency in plaques formation, which may be due to the lysis function of endolysin. There have been several reports on the isolation of phages against P. aeruginosa from sewage water [10][11][12][13][14] . However, limited reports are available on their genomic characterization, especially from India.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a Bacteriophage Infecting Pseudomonas Aeruginosa and Its Application as a Potential Decontaminating Agent

Sharma¹,

Datta²,

Chatterjee³

et al. 2021

Preprint

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To treat antibiotic resistance bacteria, bacteriophage (also called 'phage') application has recently drawn considerable attention from researchers globally. Bacteria like Pseudomonas aeruginosa are known to be associated with nosocomial infections especially in patients with compromised immune systems. In the present work, phage against P. aeruginosa (named 'DRLP1') was isolated from wastewater, enriched and characterized. Morphologically DRLP1 belongs to the family Myoviridae with a high lytic ability. DRLP1 has a burst size of approximately 100 PFU/infected cells, a rapid adsorption time when supplemented with MgCl2, and has viability in a wide temperature range and pH. Genomic sequencing and bioinformatics analysis showed that the phage genome is linear double-stranded, 66,243 bp in length and have a GC content of 54.9%. the genome encodes 93 phage related ORFs open reading frames (ORFs). Phage stability in lyophilized state, adsorption study on sodium alginate beads, and in-vitro pathogen reduction assays were also investigated. Study carried out with artificially contaminated fomites suggests that this phage has the potential for application as a biological decontaminant agent against P. aeruginosa in different conditions.

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Sewage as a rich source of phage study against Pseudomonas aeruginosa PAO

Cited by 14 publications

References 18 publications

<p>Phage therapy as a renewed therapeutic approach to mycobacterial infections: a comprehensive review</p>

<p>Phage therapy as a renewed therapeutic approach to mycobacterial infections: a comprehensive review</p>

Clinical Pharmacology of Bacteriophage Therapy: A Focus on Multidrug-Resistant Pseudomonas aeruginosa Infections

Isolation and Characterization of a Bacteriophage Infecting Pseudomonas Aeruginosa and Its Application as a Potential Decontaminating Agent

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