Sevoflurane inhibits equine myeloperoxidase release and activity in vitro

Minguet, Grégory; Rebière, Geoffroy de la; Franck, Thierry; Joris, Jean; Serteyn, Didier; Sandersen, Charlotte

doi:10.1111/j.1467-2995.2012.00786.x

Cited by 6 publications

(4 citation statements)

References 36 publications

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“…Such discrimination is important from a pathophysiological point of view because the active fraction of the enzyme is only responsible for cytotoxic injuries. 34 As previously demonstrated in animal models, 19,20 SEVO had a more important impact on the active fraction of MPO than on the total enzyme. Furthermore, the volatile anesthetic showed a different activity depending on which compounds were used to activate PMNs.…”

Section: Discussionmentioning

confidence: 64%

“…Our methodology to expose blood samples to halogenated anesthetics has already been described. 19 , 20 SEVO was administered for 1 h at concentrations of 2.3% and 4.6% to reproduce duration of anesthetic exposure and doses likely to be encountered in clinical practice. These concentrations of SEVO correspond to 1 and 2 minimum alveolar concentrations (MAC); 1 MAC (often referred to as the ED 50 ) is defined as the concentration of volatile anesthetic required to suppress movement to a surgical incision in 50% of patients.…”

Section: Methodsmentioning

confidence: 99%

“…According to previous studies in animal, 19 , 20 we estimated that 12 samples of blood (each sample drawn in a different healthy donor) would be sufficient to detect differences in ROS, total MPO, active MPO, and EL between control and SEVO-exposed blood. The experiments were conducted independently for the 12 individual samples of blood with each condition repeated in duplicate.…”

Section: Methodsmentioning

confidence: 99%

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Sevoflurane modulates the release of reactive oxygen species, myeloperoxidase, and elastase in human whole blood: Effects of different stimuli on neutrophil response to volatile anesthetic in vitro

Minguet

Franck

Joris

et al. 2017

Int J Immunopathol Pharmacol

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Volatile anesthetics have been shown to modulate polymorphonuclear neutrophil (PMN) functions. The aim of this study was to examine the impact of clinically relevant concentrations of sevoflurane (SEVO), a volatile anesthetic, on the release of reactive oxygen species (ROS), myeloperoxidase (MPO), and elastase (EL) from human activated PMNs. For this purpose, samples of whole blood were collected from healthy volunteers and exposed in vitro to 2.3% or 4.6% SEVO in air. To assess for a stimulus-dependent effect of the volatile anesthetic, PMNs were activated using different validated protocols. Artificial stimulation of neutrophils involved either a combination of cytochalasin B (CB) and N-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol 12-myristate 13-acetate (PMA). In addition, a combination of lipopolysaccharide (LPS) and tumor necrosis factor alpha (TNF-α) was also tested as a natural activation mean of PMNs. The production of ROS by PMNs was assessed by L-012 chemiluminescence. Total MPO and EL released in supernatant were measured by enzyme-linked immunosorbent assay (ELISA). Furthermore, degranulation of the active fraction of MPO was also measured by specific immunological extraction followed by enzymatic detection (SIEFED). Overall, SEVO enhanced the release of ROS, MPO, and EL following artificial stimulation of PMNs but the volatile anesthetic inhibited the degranulation of active MPO and EL after neutrophil exposure to LPS and TNF-α. This study highlighted that the effect of SEVO on activated PMNs is dependent on the conditions of cell stimulation. These properties should be taken into consideration in future studies investigating immunomodulatory effects of volatile anesthetics.

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Section: Discussionmentioning

confidence: 64%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Sevoflurane modulates the release of reactive oxygen species, myeloperoxidase, and elastase in human whole blood: Effects of different stimuli on neutrophil response to volatile anesthetic in vitro

Minguet

Franck

Joris

et al. 2017

Int J Immunopathol Pharmacol

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“…A significant decrease in stimulated neutrophil degranulation (measured by myeloperoxidase activity) was demonstrated when equine blood was exposed to 2.3% or 4.6% sevoflurane for 60 minutes in vitro (Minguet et al. ).…”

Section: Immunomodulation From Inhalation Anesthetics and Oxygenmentioning

confidence: 99%