Sevoflurane and isoflurane genotoxicity in kidney cells of mice

Brozović, Gordana; Oršolić, Nada; Rozgaj, Ružica; Knežević, Fabijan; Knežević, Anica Horvat; Maričić, Martina; Krsnik, Dajana; Benković, Vesna

doi:10.1515/aiht-2017-68-2941

Cited by 13 publications

(17 citation statements)

References 34 publications

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“…They concluded that sevoflurane was not genotoxic in vivo or in vitro. Otherside Brozovic et al [34] evaluated the DNA damage and repair in kidney cells of mice after repeated exposure to sevoflurane and isoflurane and showed that sevoflurane was slightly more genotoxic than isoflurane. Lüleci et al [31] showed that sevoflurane administration may affect cell division and have a mutagenic effect on DNA.…”

Section: Discussionmentioning

confidence: 99%

DNA damage effects of inhalation anesthetics in human bronchoalveolar cells

et al. 2019

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Background: The main objective was to evaluate and compare the local genotoxicity of sevoflurane and desflurane in bronchoalveolar cells, while the secondary outcome was to detect systemic oxidative DNA damage. To our knowledge, our study is the first one to evaluate the local effects of inhalation anesthetics in human bronchoalveolar cells in patients. Methods: American Society of Anesthesiologists group I-II patients scheduled for lumbar discectomy surgery were enrolled in this randomized prospective study. Patients were randomized to sevoflurane or desflurane for anesthesia maintenance. Bronchoalveolar lavage samples and peripheral blood samples were taken at 2-time points: the first point (baseline, T1); and the second point (postexposure, T2). Final number of 48 samples were the sevoflurane (n = 22) and desflurane (n = 26) groups. Comet assay was applied to examine genotoxic properties. Oxidative DNA damage in plasma was measured with 8-hydroxy-2′-deoxyguanosine (8-OHdG). Results: T2 values were higher than baseline values in both the desflurane group (tail-length: 66 ± 24, %DNA in tail: 72 ± 60, tail moment: 47.52 ± 14.4; P = .001, P = .005, P = .001, respectively) and the sevoflurane group (tail-length: 58 ± 33, %DNA in tail: 88 ± 80, tail moment: 51.04 ± 26.4; P = .001, P = .012, P = .001, respectively). T2 plasma 8-OHdG levels were also higher than baseline levels in the desflurane group (3.91 ± 0.19 ng/ml vs 1.32 ± 0.20 ng/ml, P = .001) and sevoflurane group (3.98 ± 0.18 ng/ml vs 1.31 ± 0.11 ng/ml, P = .001). There were no differences between the 2 groups in comet parameters and 8-OHdG levels. Conclusion: Our results indicate that both inhalation agents cause DNA damage in the bronchoalveolar cells. Also, we detected increases in plasma 8-OHdG concentrations. Local genotoxicity and systemic oxidized DNA damage were similar in both groups.

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Section: Discussionmentioning

confidence: 99%

DNA damage effects of inhalation anesthetics in human bronchoalveolar cells

et al. 2019

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“…The extent of treatment-related DNA damage was studied using the alkaline comet assay, whose usefulness has been confirmed previously in studies on rat models [25,26,27,28,29,30,31]. This assay directly measures single-, and double-strand breaks in DNA, alkali-labile sites, and single-strand breaks linked to incomplete excision repair.…”

Section: Introductionmentioning

confidence: 98%

DNA Damaging Effects, Oxidative Stress Responses and Cholinesterase Activity in Blood and Brain of Wistar Rats Exposed to Δ9-Tetrahydrocannabinol

et al. 2019

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Currently we are faced with an ever-growing use of Δ9-tetrahydrocannabinol (THC) preparations, often used as supportive therapies for various malignancies and neurological disorders. As some of illegally distributed forms of such preparations, like cannabis oils and butane hash oil, might contain over 80% of THC, their consumers can become intoxicated or experience various detrimental effects. This fact motivated us for the assessments of THC toxicity in vivo on a Wistar rat model, at a daily oral dose of 7 mg/kg which is comparable to those found in illicit preparations. The main objective of the present study was to establish the magnitude and dynamics of DNA breakage associated with THC exposure in white blood and brain cells of treated rats using the alkaline comet assay. The extent of oxidative stress after acute 24 h exposure to THC was also determined as well as changes in activities of plasma and brain cholinesterases (ChE) in THC-treated and control rats. The DNA of brain cells was more prone to breakage after THC treatment compared to DNA in white blood cells. Even though DNA damage quantified by the alkaline comet assay is subject to repair, its elevated level detected in the brain cells of THC-treated rats was reason for concern. Since neurons do not proliferate, increased levels of DNA damage present threats to these cells in terms of both viability and genome stability, while inefficient DNA repair might lead to their progressive loss. The present study contributes to existing knowledge with evidence that acute exposure to a high THC dose led to low-level DNA damage in white blood cells and brain cells of rats and induced oxidative stress in brain, but did not disturb ChE activities.

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“…Genotoxicity studies point to none to low DNA damage. In one study ( 62 ), alkaline comet assay showed no sevoflurane genotoxicity in human peripheral lymphocytes, but in another ( 63 ) DNA damage was found in kidney homogenates of rats exposed for two hours over three consecutive days. Excessive exposure in this experiment may explain why the comet tail length and intensity increased gradually until the end of experiment (24 h).…”

Section: Inhalational (Volatile) Anaestheticsmentioning

confidence: 99%

Oxidative stress under general intravenous and inhalation anaesthesia

Kundović

Rašić

Popović

et al. 2020

Archives of Industrial Hygiene and Toxicology

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Oxidative stress occurs when reactive oxygen species (ROS) production overwhelms cell protection by antioxidants. This review is focused on general anaesthesia-induced oxidative stress because it increases the rate of complications and delays recovery after surgery. It is important to know what effects of anaesthetics to expect in terms of oxidative stress, particularly in surgical procedures with high ROS production, because their either additive or antagonistic effect may be pivotal for the outcome of surgery. In vitro and animal studies on this topic are numerous but show large variability. There are not many human studies and what we know has been learned from different surgical procedures measuring different endpoints in blood samples taken mostly before and after surgery. In these studies most intravenous anaesthetics have antioxidative properties, while volatile anaesthetics temporarily increase oxidative stress in longer surgical procedures.

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Sevoflurane and isoflurane genotoxicity in kidney cells of mice

Cited by 13 publications

References 34 publications

DNA damage effects of inhalation anesthetics in human bronchoalveolar cells

DNA damage effects of inhalation anesthetics in human bronchoalveolar cells

DNA Damaging Effects, Oxidative Stress Responses and Cholinesterase Activity in Blood and Brain of Wistar Rats Exposed to Δ9-Tetrahydrocannabinol

Oxidative stress under general intravenous and inhalation anaesthesia

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